2009
DOI: 10.1111/j.1439-0272.1983.tb00167.x
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Molecular Structure of Human Seminal Coagulum: The Role of Disulfide Bonds

Abstract: taining 600 pl of 0.1 M Tris-acetate buffer, pH 7.5, in the presence or absence of 100 mM 2-mercaptoethanol. At 0,s and 15 min after incubation at room temperature, the samples

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Cited by 25 publications
(7 citation statements)
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“…Semenogelin is secreted from the seminal vesicle at ejaculation and establish major structural components of coagulated human semen. It is defined as a sperm motility inhibitor, which is classified into two types of intermediate molecular weight zinc binding protein (IMW-ZnBP), [because it has molecular weight more than 3000 and less than 80 000], the first Semenogelin I (a protein of molecular weight (MW) 52 kDa) and Semenogelin II (existed as two forms of a Sg I-related protein with MWs of 71 and 76 kDa) [48,49]. After ejaculation, serine proteases, primarily prostate-specific antigen (PSA) act to cleave SgI and SgII molecules (intermediate molecular weight zinc binding protein IMW-ZnBP) to produce soluble fragments (Low molecular weight zinc binding protein LMW-ZnBP) [50].…”
Section: Discussionmentioning
confidence: 99%
“…Semenogelin is secreted from the seminal vesicle at ejaculation and establish major structural components of coagulated human semen. It is defined as a sperm motility inhibitor, which is classified into two types of intermediate molecular weight zinc binding protein (IMW-ZnBP), [because it has molecular weight more than 3000 and less than 80 000], the first Semenogelin I (a protein of molecular weight (MW) 52 kDa) and Semenogelin II (existed as two forms of a Sg I-related protein with MWs of 71 and 76 kDa) [48,49]. After ejaculation, serine proteases, primarily prostate-specific antigen (PSA) act to cleave SgI and SgII molecules (intermediate molecular weight zinc binding protein IMW-ZnBP) to produce soluble fragments (Low molecular weight zinc binding protein LMW-ZnBP) [50].…”
Section: Discussionmentioning
confidence: 99%
“…To our knowledge, human homologs for the other identified autoantigens in rodent models of prostatitis (e.g., prostatic steroid-binding protein and spermine-binding protein) have not been identified. Although the direct human homolog of rodent SVS2 has not been identified, we focused on SVS2-like seminal vesicle proteins, such as Sg, that have previously been characterized in human seminal plasma and have the same pattern of tissue expression (36)(37)(38). In spite of the amino acid sequence disparity between SVS2 and Sg, these proteins share similarities in gene structure, 3-dimensional structure, and/or functional domains; they are collectively referred to as the rapidly evolving substrates for transglutaminase; and Sg was purified with the same approach used to purify SVS2 (24).…”
Section: Discussionmentioning
confidence: 99%
“…In the human, a chymotrypsin-like prostatic-specific antigen was determined to cause liquefaction of semen through cleavage of semenogelin I, the major component of the coagulum, which is expressed exclusively in the vesicular glands [23][24][25]. Additionally, proteolytic enzymes were detected and isolated in the seminal plasma of different species such as collagenase-like metalloproteinases in human, rat, and bull semen [26] and trypsin in semen from the guinea pig and rabbit [27,28].…”
Section: Introductionmentioning
confidence: 99%