Molecular Studies and Possible Relatedness Between R Plasmids from Groups B and D Streptococci

El-Solh, Névine; Bouanchaud, D. H.; Horodniceanu, T; Roussel, A.; Chabbert, Y

doi:10.1128/aac.14.1.19

Cited by 26 publications

(10 citation statements)

References 21 publications

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“…Transfer of R plasmids mediating multiple antibiotic resistance by conjugation was first shown by Jacob and Hobbs in S. faecalis JHI (33 (9). Transfer of resistance in vitro between streptococcal species was first described from group D to group A and B streptococci (27,29,70 (17,27,53,75,77).…”

Section: Resistance Plasmids and Interspecies Genementioning

confidence: 99%

Intergeneric and interspecies gene exchange in gram-positive cocci

Schaberg¹,

Zervos²

1986

Antimicrob Agents Chemother

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Section: Resistance Plasmids and Interspecies Genementioning

confidence: 99%

Intergeneric and interspecies gene exchange in gram-positive cocci

Schaberg¹,

Zervos²

1986

Antimicrob Agents Chemother

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“…A striking property of many MLS resistance plasmids isolated from different serogroups and of different geographical origin is their uniformity in size: 16 to 22 Md (4,5,6,9,11,12,24). The bands marked by asterisks are common bands found in digests of pRI405, pAM,81, and other MLS resistance plasmids originating from group A, B, and D streptococci (see text).…”

Section: Resultsmentioning

confidence: 99%

Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus

Engel¹,

Soedirman²,

Rost³

et al. 1980

J Bacteriol

124

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The transferability of plasmid pRI405 between various streptococci of groups A, B, and D, Streptococcus pneumoniae, and Staphylococcus aureus is described. pRI405 originated from Streptococcus faecalis and encodes for resistance to macrolides, lincomycin, and streptogramin B (MLS resistance). The host range of the well-documented streptococcal plasmid pAM/i1 was found to be similar to that of pRI405. Cleavage with restriction enzymes suggests that pRI405 belongs to a related family of MLS resistance plasmids.It is now generally accepted that resistance to various antimicrobial drugs in streptococci is mediated by plasmids. Many of these plasmids have been shown to be self-transferable (8,12,13,16,20,23,32,34) by a process that needs cellto-cell contact between donor and recipient and which resembles in several aspects conjugation (16). Non-self-transferable plasmids can be mobilized by self-transferable ones. The first reports on conjugal transfer of streptococcal plasmids involved intraspecific matings between isolates of group D streptococci (8,16,25). Later, interspecific plasmid transfer between the serogroups B and D was also reported (11, 13, 34). The plasmids used in these studies originated from group B and group D streptococci, and they encoded for resistance to macrolide antibiotics, lincomycin, and streptogramins of group B, the so-called MLS resistance phenotype (37). The host range of one particular plasmid, pAM/i1 (originating from Streptococcus faecalis), has been the subject of several studies. Le Blanc et al. (20) introduced this plasmid into a group F streptococcus by transformation and then retransferred it by mating to the oral streptococci, S. mutans, S. sanguis, and S. salivarius. Furthermore, pAM,B1 could also be transferred to members of the family of Lactobacillaceae (10). Recently, Malke (23) reported the conjugal transferability of MLS resistance plasmids originating from group A and B streptococci between streptococcal strains of groups A, D, and H. Transformation of plasmids has been demonstrated for group H and group F streptococci (11,18,19), and transduction has been reported for group A, C, and G streptococci (15,22, 29).In a preliminary study we reported intergroup transfer of an MLS resistance plasmid between streptococci of groups A, B, and D (35). This plasmid, pRI405, originated from S. faecalis. In this study we extend the earlier observations on the host range of this plasmid and show that conjugal transfer is possible also to strains of Streptococcus pneumoniae and Staphylococcus aureus.MATERIALS AND METHODS Strains. All bacterial strains used in this study are listed in Table 1. Staphylococcal phage 80 (NCTC 9788) was used in transduction experiments.Media. Streptococci, Staphylococcus aureus, and Bacillus subtilis were grown in nutrient broth or on nutrient agar as previously described (34). The media for growth of S. pneumoniae were supplemented with inactivated horse serum to a final concentration of 5%. Haemophilus influenzae was grown in nutrient broth and on nutrie...

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“…A number of these have been transferred, by conjugation, to different species of Streptococcus and to S. aureus (7). DNA-DNA homology studies (12,15,41) and restriction endonuclease analyses (17) have suggested that these plasmids are closely related. Two independently isolated MLS plasmids, pAC1 from Streptococcus pyogenes and pAM,B1, were virtually identical (41).…”

mentioning

confidence: 99%

Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region

LeBlanc¹,

Lee²

1984

J Bacteriol

104

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Plasmid pAM,1, originally isolated from Streptococcus faecalis DS5, mediates resistance to the MLS (macrolide, lincosamide, and streptogramin Ba) group of antibiotics. A restriction endonuclease map of the 26.5-kilobase (kb) pAM31 molecule was constructed by using the enzymes AvaI, HpaII, EcoRI, PvuII, KpnI, BstEII, HpaI, HhaI, and HindIII. A comparison of this map to those of four independently isolated deletion derivatives of pAMP1 located the MLS resistance determinant within a 2-kb DNA segment and at least one conjugative function within an 8-kb region. The 5.0-kb EcoRI-B fragment from pAM,1 was ligated onto the 4.0-kb Escherichia coli plasmid vector, pACKC1, and used to transform E. coli HB101. The 9.0-kb chimeric plasmid was then used to transform Streptococcus sanguis Challis with concurrent expression of the E. coli kanamycin resistance determinant. The 5.0-kb EcoRI-B fragment from pAMP1 was subsequently used as a vector to clone a streptomycin resistance determinant from a strain of Streptococcus mutans containing no detectable plasmid DNA. Subcloning experiments, using a Hindlll partial digest of pAM,1 DNA, narrowed the replication region of this plasmid to a 2.95-kb fragment.Plasmid pAM31, originally identified in a clinical isolate of Streptococcus faecalis DS5 (8), mediates resistance to erythromycin, which is representative of the MLS phenotype (resistance to macrolides, lincosamides, and streptogramin Ba; 40). Plasmid pAMP1 was transformed into the Challis on August 5, 2020 by guest

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Molecular Studies and Possible Relatedness Between R Plasmids from Groups B and D Streptococci

Cited by 26 publications

References 21 publications

Intergeneric and interspecies gene exchange in gram-positive cocci

Intergeneric and interspecies gene exchange in gram-positive cocci

Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus

Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region

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