Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt

Abstract

The current study was conducted on 210 random samples of meat products (beef burger, kofta, luncheon, minced meat, sausage) and diarrheic human stool of patients suffering from vomiting and diarrhea (35 for each). The meat products were collected from different shops and hospitals at Kaliobia Governorate, Egypt, for detection of B. cereus strains, and their phenotypic characterization as well as antibiotic resistant genes. Bacteriological examination of the collected samples indicated the identification of 51 (24.3%) isolates of B. cereus from 210 samples as 11 (31.4%) from kofta 13 (37.1%) from minced meat, 9 (25.7%) from sausage, 7 (20.0%) from beef burger, 6 (17.1%) from luncheon samples, and 5 (14.3%) from human stool specimens. Most of 51 isolated B. cereus strains had the ability for biofilm production. The antibiotic sensitivity profiles revealed that the isolated B. cereus was highly resistant for Penicillin-G followed by methicillin, ampicillin, oxytetracycline, sulfatrimethoprim and cefotaxime. Meanwhile, they were highly sensitive to gentamycin and norfloxacin followed by ciprofloxacin, meropenem and florphenicol. Further, PCR declared that bla, tetA and erm genes were amplified in 9, 7, 6 out of 10 studied B. cereus isolates giving products of 680 bp, 502 bp, and 645 bp, respectively. Therefore, one can conclude that B. cereus, especially antibiotic resistances ones, is meat-borne pathogens of public health importance and they may be the causative agents in patients suffering from vomiting and diarrhea.

Molecular characterization and prevalence of Bacillus species isolated from Saudi hospitals

“…With regards to sulfamethoxazole-trimethoprim, this study revealed a high rate of susceptibility among Bacillus isolates (96.6%). This finding is not in agreement with a previous study 21 which reported an intermediate susceptibility rate of 80.4% for sulfamethoxazole-trimethoprim In the present study, 45 different resistotypes were detected. In addition, 21.6% of the total Bacillus isolates were MDR and belonged to 22 distinct resistotypes.…”

“…In the current study, high susceptibility was detected for both ciprofloxacin (97.9%) and gentamicin (97.2%). This finding agrees with a previous study 21 which reported a susceptibility rate of 74.5% for ciprofloxacin and 82.3% for gentamicin. With regards to sulfamethoxazole-trimethoprim, this study revealed a high rate of susceptibility among Bacillus isolates (96.6%).…”

Newly Emerging MDR B. cereus in Mugil seheli as the First Report Commonly Harbor nhe, hbl, cytK, and pc-plc Virulence Genes and bla1, bla2, tetA, and ermA Resistance Genes

“…Nearly similar findings were reported by Savić 67 and Mousa. 68 The present findings affirm the emergence of MDR isolates of B. cereus in aquaculture sectors and set the fact that the food chain can be a major route of transmission of MDR bacteria between animals and humans. 69 The random use of antibiotics to treat emerging diseases has dire consequences in the aquaculture industry, as it may lead to the existence of MDR strains.…”

Evaluation of Bacterial Contamination From Raw and Cooked Fish, Mutton and Beef Sold by Local Vendors in Hyderabad, Pakistan

“…Our results are agreed with Gamal et al (2020) who reported that B.cereus isolates were susceptible to gentamycin, norfloxacin, and ciprofloxacin; while resistant against ampicillin, oxytetracycline, streptomycin, neomycin and erythromycin. Mousa et al (2020) described antibiotic resistant B.cereus in Kaliobia Egypt and reported 82.3% sensitivity to both gentamycin and norfloxacin, 74.5% to ciprofloxacin and resistant against ampicillin, and oxytetracycline; whereas intermediate sensitivity showed against streptomycin, erythromycin and neomycin.…”