The current study was conducted on 210 random samples of meat products (beef burger, kofta, luncheon, minced meat, sausage) and diarrheic human stool of patients suffering from vomiting and diarrhea (35 for each). The meat products were collected from different shops and hospitals at Kaliobia Governorate, Egypt, for detection of B. cereus strains, and their phenotypic characterization as well as antibiotic resistant genes. Bacteriological examination of the collected samples indicated the identification of 51 (24.3%) isolates of B. cereus from 210 samples as 11 (31.4%) from kofta 13 (37.1%) from minced meat, 9 (25.7%) from sausage, 7 (20.0%) from beef burger, 6 (17.1%) from luncheon samples, and 5 (14.3%) from human stool specimens. Most of 51 isolated B. cereus strains had the ability for biofilm production. The antibiotic sensitivity profiles revealed that the isolated B. cereus was highly resistant for Penicillin-G followed by methicillin, ampicillin, oxytetracycline, sulfatrimethoprim and cefotaxime. Meanwhile, they were highly sensitive to gentamycin and norfloxacin followed by ciprofloxacin, meropenem and florphenicol. Further, PCR declared that bla, tetA and erm genes were amplified in 9, 7, 6 out of 10 studied B. cereus isolates giving products of 680 bp, 502 bp, and 645 bp, respectively. Therefore, one can conclude that B. cereus, especially antibiotic resistances ones, is meat-borne pathogens of public health importance and they may be the causative agents in patients suffering from vomiting and diarrhea.

The study was performed on 175 random samples of fresh broiler chicken and chicken meat products viz: Chicken burger; chicken luncheon; chicken kofta and chicken sausage (35 for each), collected from different shops at Benha city, kaliobia government. The sample were examined for detection the prevalence of Staphylococci with special reference to S. aureus and MRSA, beside the phenotypic characterization of the isolated S. aureus strains and detection of their virulence genes in them. The bacteriological examination revealed the isolation of 98 Staphylococcus species including 41 S.aureus; 54 S. epidermidis and 3S. chromogenes. In addition, all 41 isolated S. aureus were coagulase positive strains, while, the other isolated Staphylococcal strains (57) were coagulase negative ones. Moreover, the results of SET-RPLA test revealed that 5 S. aureus strains out of 10 randomly examined strains were enterotoxigenic and classified according to type of toxin into (3A; 1 B & 1C). The sensitivity tests for the isolated S. aureus showed strains indicated high resistance to methicillin followed by oxacillin; Nalidixic acid; Ampicillin; Amoxicillin; Cefotaxime and Tobramycin, respectively. Meanwhile, they were highly sensitive to Gentamycin; Enrofloxacin; Norfloxacin; Lomefloxacin and Ciprofloxacin. PCR results cleared that, femA; mecA and hlb virulence genes were detected in all 5 S. aureus studied strains. Meanwhile, ica A was detected in 4studied strains; enterotoxin A (sea) was detected in 2 studied strains chicken meat and chicken kofta samples only and leukocidin (pvl) virulence gene was detected in 1 studied strain of chicken meat sample.