Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe

Criado–Fornelio, A.; Martı́nez-Marcos, Alino; Buling-Saraña, A.; Barba-Carretero, J.C.

doi:10.1016/s0304-4017(03)00141-9

Cited by 201 publications

(154 citation statements)

References 36 publications

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“…In Hungarian dogs, only Babesia canis canis has been detected (Földvári et al 2005b). Recent studies using molecular methods showed that in France, Slovenia and Spain, where R. sanguineus and D. reticulatus coexist, both Babesia canis canis and Babesia canis vogeli were detected (Cacciò et al 2002, Criado-Fornelio et al 2003b, Duh et al 2004. Small canine piroplasms also occur in Europe.…”

Section: Introductionmentioning

confidence: 90%

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Hard Ticks Infesting Dogs in Hungary and their Infection with Babesia and Borrelia Species

et al. 2007

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A survey was carried out in Hungary to investigate the occurrence of hard tick species (Acari: Ixodidae) collected from dogs and Borrelia and Babesia spp. detected in them. In total, 1,424 ticks were removed from 477 dogs appearing for clinical consultation in veterinary practices and clinics countrywide. Ixodes ricinus and Dermacentor reticulatus were the most common species occurring in most of the studied areas. Females of these two species were selected for molecular analyses. One to twelve specimens were used in each sample for DNA extraction. Polymerase chain reactions were performed with BSL-F/BSL-R primers for detecting Borrelia spp. in I. ricinus and with PIRO-A1/PIRO-B primers to amplify Babesia spp. DNA in D. reticulatus. Randomly selected PCR products were sequenced to identify the pathogens' species or subspecies. DNA of Borrelia spp. could be detected in six (5.6 %) from 108 I. ricinus samples and 43 (29.9 %) from 144 D. reticulatus samples were PCR-positive for Babesia spp. Sequencing revealed the highest similarity with Borrelia afzelii, Borrelia garinii and Babesia canis canis, respectively. Babesia and Borrelia spp. were identified in ticks with molecular methods for the first time in Hungary, and a high prevalence of B. canis canis in D. reticulatus females collected from dogs was detected.

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Section: Introductionmentioning

confidence: 90%

“…Theileria equi and Theileria annulata are probably also capable of infecting dogs, since they were detected in symptomatic animals (Criado-Fornelio et al 2003b, Criado et al 2006.…”

Section: Introductionmentioning

confidence: 98%

Hard Ticks Infesting Dogs in Hungary and their Infection with Babesia and Borrelia Species

et al. 2007

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“…Molecular PCR methods are more sensitive and specific for pathogens in peripheral blood and arthropod vectors than other methods [2,8]. Moreover, PCR analysis followed by sequence analysis can be used for phylogenic characterization of Hepatozoon isolates [2]. These molecular methods can play an important role in the diagnosis of canine hepatozoonosis and research into its distribution and prevalence.…”

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confidence: 99%

“…Recently, molecular methods have been used to detect Hepatozoon spp. Molecular PCR methods are more sensitive and specific for pathogens in peripheral blood and arthropod vectors than other methods [2,8]. Moreover, PCR analysis followed by sequence analysis can be used for phylogenic characterization of Hepatozoon isolates [2].…”

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A PCR-Based Epidemiological Survey of Hepatozoon canis in Dogs in Nigeria

Sasaki

Omobowale

Ohta

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The prevalence of Hepatozoon canis infections in dogs in Nigeria was surveyed using molecular methods. DNA was extracted from blood samples obtained from 400 dogs. A primer set that amplified the Babesia canis 18S rRNA gene, which has high similarity to the H. canis 18S rRNA gene, was used for the PCR. As a result, samples from 81 dogs (20.3%) produced 757 bp bands, which differed from the 698 bp band that corresponded to B. canis infection. The sequence of the PCR products of 10 samples were determined, all of which corresponded with the H. canis sequence.

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Diagnosis of Babesia caballi and Theileria equi infections in horses in Sudan using ELISA and PCR

Salim

Hassan

Bakheit³

et al. 2008

Parasitol Res

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The purpose of this study was to estimate the prevalence of equine piroplasmosis in Sudan. The presence of antibodies against Babesia caballi and Theileria equi was determined in serum samples obtained from 158 horses raised in different locations in Sudan by enzyme-linked immunosorbent assay (ELISA). The B. caballi 48-kDa and the T. equi EMA-2 purified recombinant proteins were used as antigens in the ELISA test. Results showed that seven (4.4%) were positive for B. caballi and 80 (63.5%) were positive for T. equi. Polymerase chain reaction (PCR) assays have been applied using primers targeting the B. caballi 48-kDa merozoite antigen, the T. equi SSUrRNA and the T. equi EMA-1 genes. PCR performed on 131 blood spots in filter paper revealed that 33 (25.2%) samples were positive for T. equi but no positives were found for B. caballi. It is concluded that equine piroplasmosis is endemic in the country. This is the first study on serological and molecular epidemiological diagnosis on equine piroplasmosis in Sudan.

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Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe

Cited by 201 publications

References 36 publications

Hard Ticks Infesting Dogs in Hungary and their Infection with Babesia and Borrelia Species

Hard Ticks Infesting Dogs in Hungary and their Infection with Babesia and Borrelia Species

A PCR-Based Epidemiological Survey of Hepatozoon canis in Dogs in Nigeria

Diagnosis of Babesia caballi and Theileria equi infections in horses in Sudan using ELISA and PCR

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