Molecular Study and Antibiotic susceptibility patterns of some Extended Spectrum Beta-Lactamase Genes (ESBL) of Klebsiella pneumpniae in Urinary Tract Infections

Abstract

The present study included the collection of 121 samples from MSU for investigating the presence of K.pneumoniae in UTIs, the samples have been collected from Al-Shaheed Mohammed Baqir AL-Hakeem hospital and private laboratories in Baghdad province. The study was carried out through March 2019 to the beginning of June 2019. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing on number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to K.pneumoniae bacteria during previous tests based on the blaTEM, blaSHV and blaCTX-M genes which has specific sequences for K.pneumoniae bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance. The results of the final diagnosis showed that 38 isolates belong to target bacteria, The examination of the sensitivity of all bacterial isolates was done for selected 38 isolation towards the 16 antibiotics by a Vitek2 compact ASTN system and the isolates were resistant for a number of antibiotics used such as; Amikacin (5.26%), Imepenem (5.26%), Ertapenem (7.89%), Meropenem (10.52%), Gentamicin (21.05%), Ciprofloxacin (26.32%), Cefoxitin (39.47%), Trimethoprim/Sulfamethoxazole (50%), Ceftriaxone (52.63%), Fosfomycin (55.26%), Piperacillin/Tazobactam (57.89%), Nitrofurantoin (57.89%), Ceftazidime (65.79%), Cefuroxime (71.05%), Cefixime (73.68%) and Ampicillin (100%). The presence of Extended Spectrum Beta-Lactamase genes in 38 K.pneumoniae isolates were 65.8 % of the ESBL genotypes expressed blaSHV genes followed by 52.6 % blaTEM and 42.1 % for blaCTX-M.

In Vitro Control of Multidrug-Resistant Klebsiella pneumoniae Infection by Some Biocides used in Erbil Hospitals

“…Another study from Erbil (Tawgozy and Amin, 2018), Duhok (Naqid et al, 2020), Baghdad (Al-Hashimy and Al-Musawy, 2020), Russia (Khaertynov et al, 2018), andIran (Kashefieh et al, 2021) found comparable results. While imipenem and meropenem, followed by amikacin and Ciprofloxacin, have demonstrated good activity and effectiveness against K. pneumoniae, this finding is consistent with a study conducted in Erbil by (Ali and Ismael, 2017). Furthermore, another review from China (Effah et al, 2020) observed a similar issue.…”

“…On the other hand, a study by (Alcántar-Curiel et al, 2018) suggested that while it appears that antibiotic resistance and the bacterial ability to form biofilm play a significant role in the worldwide spread of K. pneumoniae, the unambiguous relationship between these elements has not been fully recognized and expanded.…”

Extended-spectrum beta-lactamases encoding genes among <i>salmonella enterica</i> serovar typhi Isolates in patients with typhoid fever from four Academic Medical Centers in Lagos, Nigeria

“…The detection of bla TEM , bla CTX-M , and bla SHV was done by multiplex PCR (M-PCR) using specific primers (Promega Corp, Germany) as previously published (Table 1) 21,22 . Each PCR reaction included a 20 μL volume, comprising 10 μL of ×1 PCR buffer (pH 8.3), 1.5 nm of MgCl 2 , 200 nM each of the deoxynucleotide triphosphates, 0.5 μL of 40 pmol each of the forward and reverse primers, 1 μL of genomic DNA template (~100 ng), 1.25 U of Taq polymerase (Promega Corp, Germany), and DNA/RNA free water up to 20 μL.…”