The present study included the collection of 121 samples from MSU for investigating the presence of K.pneumoniae in UTIs, the samples have been collected from Al-Shaheed Mohammed Baqir AL-Hakeem hospital and private laboratories in Baghdad province. The study was carried out through March 2019 to the beginning of June 2019. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing on number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to K.pneumoniae bacteria during previous tests based on the blaTEM, blaSHV and blaCTX-M genes which has specific sequences for K.pneumoniae bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance. The results of the final diagnosis showed that 38 isolates belong to target bacteria, The examination of the sensitivity of all bacterial isolates was done for selected 38 isolation towards the 16 antibiotics by a Vitek2 compact ASTN system and the isolates were resistant for a number of antibiotics used such as; Amikacin (5.26%), Imepenem (5.26%), Ertapenem (7.89%), Meropenem (10.52%), Gentamicin (21.05%), Ciprofloxacin (26.32%), Cefoxitin (39.47%), Trimethoprim/Sulfamethoxazole (50%), Ceftriaxone (52.63%), Fosfomycin (55.26%), Piperacillin/Tazobactam (57.89%), Nitrofurantoin (57.89%), Ceftazidime (65.79%), Cefuroxime (71.05%), Cefixime (73.68%) and Ampicillin (100%). The presence of Extended Spectrum Beta-Lactamase genes in 38 K.pneumoniae isolates were 65.8 % of the ESBL genotypes expressed blaSHV genes followed by 52.6 % blaTEM and 42.1 % for blaCTX-M.

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).