Molecular Subtyping and Antimicrobial Susceptibilities ofCampylobacter coliIsolates from Diarrheal Patients and Food-Producing Animals in China

Abstract

The aim of this study was to determine the molecular subtyping and antimicrobial susceptibility characteristics of Campylobacter coli isolates from different sources in China. One hundred thirteen C. coli isolates were subtyped by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and porA and flaA short variable region (SVR) nucleotide sequences. Cluster analysis was performed based on the PFGE and sequence types (ST). Eighty-four PFGE patterns (SmaI) were observed in 113 isolates. Fifty-four STs (28 novel) and three clonal complexes (CC), 86% of which were clustered to CC828, were observed, as well as 52 porA and 37 flaA-SVR sequence alleles. MLST, porA, and flaA-SVR analysis demonstrated that many isolates from diarrheal patients shared identical genotypes with chicken isolates. Minimum inhibitory concentration values of 10 antibiotics were analyzed for 109 isolates isolated in 2011 using the E-test method. The most frequently observed resistance agents were nalidixic acid (100%) and ciprofloxacin (100%), followed by levofloxacin (99%), tetracycline (94%), metronidazole (93%), erythromycin (61%), streptomycin (72%), gentamicin (59%), ampicillin (50%), and chloramphenicol (29%). Multidrug resistance was detected in 108 of 109 C. coli isolates (99%).

Molecular Characterization, Antimicrobial Resistance and Caco-2 Cell Invasion Potential of Campylobacter jejuni/coli from Young Children with Diarrhea

“…Because of the lack of large enough data to be representative of the diversity of C. jejuni in China, more studies need to be carried out to determine such differences. Most C. coli isolates belonged to ST-828 complex in our study, which correlated well with other studies in China, 39 South Korea, 37 America, 40 Portugal 41 and the Mediterranean area. 42 Surveillance programs monitoring antimicrobial resistance of Campylobacter and other foodborne pathogens have been established in several countries.…”

“…25 In addition, GEN, CLI and CHL are also used for antibiotic therapy. Antimicrobial resistance rates were also high in C. coli (73.3%, 63.3% and 20%, respectively), which was similar with the results of another study in north China 39 and Korea, 38 suggesting the emergence of resistance to these antimicrobials. Therefore, establishment of integrated surveillance networks in China and other countries should be taken into consideration.…”

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

“…The existence of high genotypic similarity amongst some strains isolated in Brazil is in contrast with previous studies conducted in several countries that showed high genomic diversity amongst C. coli strains (Harrington et al, 1997;Magnú sson et al, 2011;Giacomelli et al, 2012;Zhang et al, 2014). …”

Functional genomics in Campylobacter coli identified a novel streptomycin resistance gene located in a hypervariable genomic region

“…In recent years, frequent occurrences of aminoglycoside resistant Campylobacter isolates of animal and human origin have been reported in several countries (Guevremont et al, 2006;Pezzotti et al, 2003;Qin et al, 2011;Torralbo et al, 2015;Zhang et al, 2014), and in some studies, higher rates of streptomycin (STR) resistance have been detected in C. coli compared to C. jejuni (Giacomelli et al, 2014;Torralbo et al, 2015). Several mechanisms of aminoglycoside resistance have been described in Gram-positive and Gram-negative bacteria with the most important ones being alterations at the ribosomal binding sites (Carter et al, 2000;Meier et al, 1996;Olkkola et al, 2010;Torii et al, 2003) and enzymatic drug modification (for review see Ramirez & Tolmasky, 2010).…”