Molecular Subtyping of Treponema pallidum Subspecies pallidum

Abstract

This is the first molecular subtyping system that distinguishes among clinical isolates of T. pallidum.

Modern aspects of epidemiological surveillance of gonococcal infection spread

“…Опубликованы данные о широком распространении двух субтипов ВИЧ-1 в Таиланде [88], преобладании субтипа А ви-руса иммунодефицита человека 1-го типа в популя-ции с высокой превалентностью ВИЧ в Северной Америке (Нью-Йорк) [89], о результатах изучения методами молекулярной эпидемиологии путей рас-пространения субтипов ВИЧ-1 B и Е [90], Chlamydia trachomatis [91], Treponema pallidum [92][93][94]. По мне-нию авторов проведенных исследований, примене-ние методов молекулярного типирования способ-ствует пониманию закономерностей распростране-ния генотипов возбудителя между регионами одной страны и странами мира, изучению биологических особенностей возбудителей, циркулирующих в раз-ных географических регионах, в том числе установ-лению возможной связи субтипов с вирулентно-стью, определению особенностей передачи инфек-ции между половыми партнерами.…”

Whole Genome Analyses of Treponemes: New Targets for Strain- and Subspecies-Specific Molecular Diagnostics

“…In the Nichols genome, this insertion was found only in part of the treponemal population (Šmajs et al, 2002). With regard to the arp gene (Pillay et al, 1998), a variable number of tandem repetitions were found in the tested genomes. Based on amino acid variations, previously published papers (Harper et al, 2008b;Liu et al, 2007) classified the TPA and TPE Arp repeat motifs into 4 types (I, II, III, II/III), and the variability in repeat sequence types correlated with the sexual transmission strategy (Harper et al, 2008b).…”

“…The Tpr proteins are heterogenous proteins considered as potential virulence factors involved in pathogenesis and/or immune evasion (Giacani et al, 2004;Gray et al, 2006), and in inducing an antibody response during treponemal infection (Centurion-Lara et al, 1999, 2000a, 2000b. Other differences between TPA and TPE involved a Mcp (methyl-accepting chemotaxis protein, TPE_0488), three treponemal antigens (TPE_0136; (Brinkman et al, 2008), the Tp92 -outer membrane protein (TPE_0326; Cameron et al, 2000), the Arp protein (TPE_0433, Pillay et al, 1998)) and an elongated RecQ protein (TPE_0103) (Čejková et al, unpublished results).…”

“…Different sequences at a particular locus are denoted with letters a-d. **nd, not determined ***sensitive, unmutated 23S rRNA resulting in susceptibility of TPA strains to macrolide antibiotics; A2058G or A2059G mutations result in resistance to macrolides. ****d, restriction patterns according to Pillay et al, (1998) The data shown in Table 5 indicate numbers of different genotypes identified by sequencing analysis of several treponemal chromosomal loci. The greatest numbers of genotypes were found for the locus TP0548 (5) and for genetic variants detected by amplification of the arp gene (5).…”

Sexually transmitted diseases: Impact of molecular laboratory diagnostic methods on disease control and prevention

“…Thus, there is ample time to identify sexual contacts and treat them to interrupt the chain of transmission. To understand the patterns and dynamic of disease transmission, molecular typing methods are being developed (15). These typing schemes will be useful in the construction of accurate spread networks for the sexual transmission of disease and will be important tools for outbreak investigation and control.…”