Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe

Rudolf, Ivo; Betášová, Lenka; Bischof, Vlastimil; Venclíková, Kristýna; Blažejová, Hana; Mendel, Jan; Hubálek, Zdeněk; Kosoy, Michael

doi:10.1007/s00436-016-5175-2

Cited by 26 publications

(27 citation statements)

References 19 publications

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“…However, two studies reported a failure to detect Rickettsia spp . in M. ovinus collected in Ethiopia and the Czech Republic [ 18 , 47 ]. Using next-generation sequencing, we similarly were unable to detect Rickettsia in two groups of M. ovinus collected from Jiuquan in Gansu Province, China.…”

Section: Discussionmentioning

confidence: 99%

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Microbial population analysis of the midgut of Melophagus ovinus via high-throughput sequencing

Duan

Cheng

et al. 2017

Parasites Vectors

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Background Melophagus ovinus, one of the most common haematophagous ectoparasites of sheep, can cause anaemia and reductions in weight gain, wool growth and hide value. However, no information is available about the microfloral structure of the midgut of this ectoparasite. In the present study, we investigated the microbial community structure of the midgut contents of fully engorged female and male M. ovinus using Illumina HiSeq.ResultsThe phylum showing the highest abundance was Proteobacteria (99.9%). The dominant bacterial genera in females and males were Bartonella, Arsenophonus and Wolbachia. Some less abundant bacterial genera were also detected, including Enterobacter, Acinetobacter, Halomonas, Shewanella, Bacillus and Staphylococcus.Conclusions Bartonella, Arsenophonus and Wolbachia were the dominant bacterial genera in the midgut of female and male M. ovinus. Although detected, Enterobacter, Acinetobacter, Halomonas, Shewanella, Bacillus and Staphylococcus showed low abundances. Importantly, this is the first report of the presence of Arsenophonus, Wolbachia, Enterobacter, Halomonas, Shewanella, Bacillus and Staphylococcus in the midgut of M. ovinus.

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Section: Discussionmentioning

confidence: 99%

“…Similarly, Nelder et al [ 17 ] screened a 150 M. ovinus individuals for Coxiella burnetii , with negative results. Rudolf et al [ 18 ] also failed to find evidence of flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi , Rickettsia spp., Anaplasma phagocytophilum or Babesia spp. in M. ovinus .…”

Section: Introductionmentioning

confidence: 99%

Microbial population analysis of the midgut of Melophagus ovinus via high-throughput sequencing

Duan

Cheng

et al. 2017

Parasites Vectors

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“…Recently, two reports have shown the absence of Rickettsia spp. in M. ovinus collected in Ethiopia and the Czech Republic [13, 33]. …”

Section: Discussionmentioning

confidence: 99%

First report of Rickettsia raoultii and R. slovaca in Melophagus ovinus, the sheep ked

et al. 2016

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Background Melophagus ovinus (Diptera: Hippoboscidae), a hematophagous ectoparasite, is mainly found in Europe, Northwestern Africa, and Asia. This wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. Furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. Herein, we investigated the presence of various Rickettsia species in M. ovinus.MethodsIn this study, a total of 95 sheep keds were collected in Kuqa County and Alaer City southern region of Xinjiang Uygur Autonomous Region, northwestern China. First, collected sheep keds were identified on the species level using morphological keys and molecular methods based on a fragment of the 18S ribosomal DNA gene (18S rDNA). Thereafter, to assess the presence of rickettsial DNA in sheep keds, the DNA of individual samples was screened by PCR based on six Rickettsia-specific gene fragments originating from six genes: the 17-kilodalton antigen gene (17-kDa), 16S rRNA gene (rrs), surface cell antigen 4 gene (sca4), citrate synthase gene (gltA), and outer membrane protein A and B genes (ompA and ompB). The amplified products were confirmed by sequencing and BLAST analysis (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome).ResultsAccording to its morphology and results of molecular analysis, the species was identified as Melophagus ovinus, with 100% identity to M. ovinus from St. Kilda, Australia (FN666411). DNA of Rickettsia spp. were found in 12 M. ovinus samples (12.63%, 12/95). Rickettsia raoultii and R. slovaca were confirmed based on phylogenetic analysis, although the genetic markers of these two rickettsial agents amplified in this study showed molecular diversity.ConclusionsThis is the first report of R. raoultii and R. slovaca DNA in M. ovinus. Rickettsia slovaca was found for the first time around the Taklimakan Desert located in China. This finding extends the geographical range of spotted fever group rickettsiae. Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1885-7) contains supplementary material, which is available to authorized users.

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“…Assessing the microbial community of insect vectors could facilitate further studies with respect to how the microbiota interacts with vectors of animal parasites, as well as how to develop tools for biological control. There have been studies on the characteristics of microbial populations in the midgut of the male and female M. ovinus (Duan et al ., ), including comprehensive investigations of arboviral, bacterial and protozoan vector‐borne pathogens within M. ovinus (Rudolf et al ., ), as well as the characteristics of symbiotic systems associated with M. ovinus (Nováková et al ., ). However, the bacterial community in insects is not always stable.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of microbial community in the whole body and midgut from fully engorged and unfed female adult Melophagus ovinus

Duan

Zhou

Cheng

2019

Medical Vet Entomology

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Melophagus ovinus is a type of ectoparasite infesting sheep. Data regarding the comprehensive bacterial community associated with the whole body and midgut of M. ovinus under different engorged statuses are required. Melophagus ovinus were collected from the city of Jiuquan, China. Bacterial DNA was extracted from the whole body and midgut of fully engorged female adults, or newly hatched and unfed adult female M. ovinus. The 16S rRNA gene V3-V4 hypervariable regions were sequenced using the IonS5™XL platform (Thermo Fisher Scientific, Waltham, MA, U.S.A.). The whole body bacterial diversity of the newly hatched, unfed adult females was greater compared with that of the other three samples. Proteobacteria was the dominant bacterial phylum in all of the samples. Of the 42 total bacterial genera present in all of the experimental samples, Arsenophonus, Bartonella and Wolbachia were the dominant genera. The relative abundance of Arsenophonus in midgut was greater than that in the whole body. The relative abundance of Bartonella in fully engorged adults was far greater than those in newly hatched, unfed adults. The relative abundance of Wolbachia was highest in the whole body of newly hatched, unfed adults. Seventeen bacterial species were identified in all experimental samples. Bartonella chomelii, Streptococcus hyointestinalis and Escherichia coli were the first species reported in M. ovinus.

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Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe

Cited by 26 publications

References 19 publications

Microbial population analysis of the midgut of Melophagus ovinus via high-throughput sequencing

Microbial population analysis of the midgut of Melophagus ovinus via high-throughput sequencing

First report of Rickettsia raoultii and R. slovaca in Melophagus ovinus, the sheep ked

Comparative analysis of microbial community in the whole body and midgut from fully engorged and unfed female adult Melophagus ovinus

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