Molecular Symbiosis of CHOP and C/EBPβ Isoform LIP Contributes to Endoplasmic Reticulum Stress-Induced Apoptosis

Indomethacin suppresses LAMP-2 expression and induces lipophagy and lipoapoptosis in rat enterocytes via the ER stress pathway

“…5a, immunofluorescence staining showed that the CHOP expression was very low at 0 h of indomethacin treatment; however, there was a marked increase in the nuclear expression of CHOP, which peaked at 6 h and was maintained to 24 h at a lower level. Additionally, many CHOP-expressing enterocytes at 24 h underwent apoptosis, which may be induced by prolonged ER stress [9,37]. The results of immunofluorescence staining were confirmed by Western blot analysis (Fig.…”

“…This decrease of LAMP-2 expression was associated with accumulation of ALPs and LDs in treated enterocytes and elevation of lipoapoptosis [9][10][11][12], which peaked at 24 h.The [50,51], lipid-mediated alterations of lysosomal membranes [13,52], or other mechanisms. The results of this in vitro study clearly indicate that the signaling pathway of indomethacin-induced lipophagy and apoptosis in enterocytes is mainly mediated by ER stress (confirmed by blocking ER stress with 4-PBA) and suppression of mTOR [36][37][38][39][40]. Lipids are physiological ER stressors [53]; thus, there is a possibility of LD-induced ER stress in indomethacin-treated enterocytes.…”

“…The autophagic and apoptotic responses of cultured enterocytes to indomethacin treatment are mediated by ER stress and mTOR inhibition Autophagy and apoptosis could be induced by ER stress and mTOR inhibition [36][37][38][39][40]. Thus, we examined the expression of the ER stress marker CHOP at different time points.…”

GCN2-Dependent Metabolic Stress Is Essential for Endotoxemic Cytokine Induction and Pathology

“…6F). However, we did not see the preferential association with either inhibitory or activating isoforms that had been described in previous studies using various cell lines (34,35,38). Nevertheless, the data support the hypothesis that CHOP may enhance IL-6 production by interacting with C/EBP␤.…”

A Self-defeating Anabolic Program Leads to β-Cell Apoptosis in Endoplasmic Reticulum Stress-induced Diabetes via Regulation of Amino Acid Flux

“…Other Methods-The following techniques were performed as described: cell extraction and Western blotting (17), using antibodies listed in the figure legends; incorporation of [ 35 S]Met/Cys using EXPRE 35 S 35 S Protein Labeling Mix (18); eIF2B-GEF activity (19); and flow cytometry (17). Charged and total tRNA species were quantified as described (20).…”