Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov.

Abstract

The nucleotide sequences of 16S ribosomal-RNA of Streptococcus uberis types I and II were determined by reverse transcriptase. Comparative analysis of the sequence data showed that the two types are phylogenetically distinct and worthy of separate species status. A new species Streptococcus parauberis is proposed for the type II strains. The type strain of Strep. parauberis is NCDO 2020.

Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis

“…According to Bentley et al (4) and Bentley and Leigh (6), the sequence variability of the V2 region of the 16S rRNA allows a differentiation of 31 species of the genus Streptococcus, including the species S. uberis and S. parauberis. Both species, formerly classified as S. uberis types I and II (19), are well known as causative agents of bovine mastitis. According to biochemical and serological characteristics, the two species are almost indistinguishable (19).…”

“…Both species, formerly classified as S. uberis types I and II (19), are well known as causative agents of bovine mastitis. According to biochemical and serological characteristics, the two species are almost indistinguishable (19). A molecular identification of both species can be performed by analysis of restriction fragment length polymorphisms (RFLPs) of the 16S rRNA gene (13,14,18) or by the use of species-specific oligonucleotide probes (5,6).…”

Antigenic and genetic homogeneity ofStreptococcus uberisstrains from the bovine udder

“…11) S. durans (12). S. boris strain WVINTI (13) (17,18) and one S. zymoyenes strain (19). The antigens were separated by SDS-PAGE (120%) and immunoblotted with rabbit antiserum R2 to S. uberis, followed by incubation with peroxidase-conjugated goat anti-rabbit serum and protein The reaction was developed with 4-chloro-1 -napthol.…”

“…We therefore support the introduction of S. uberis subtype II as S. parauberis sp. nov. as recently proposed following comparative analysis of 16S ribosomal RNA sequences [13] [27], coagulasenegativer and methicillin-resistant staphylococci [28], Staphylococc as aare as [29,30], Clampylobacter pylori [31], Clandida albican.8 [32] and Clo.stridium difflcile [33,34]. Immunoblot [36].…”

“…Recently, comparative analysis of the 16S ribosomal RNA sequences of a S. uberis subtype I and a subtype II strain disclosed important differences [13] and have been the basis of a proposal that subtype II be designated S. parauberis, a new species with strain NCDO 2020 as the type strain. S. parauberis can only be distinguished from S. uberis by its ability to grow at 10°C, but not by cultural morphology or biochemical properties.…”