Molecular taxonomy and phylogenetic inferences of Bichromomyia flaviscutellata complex based on the COI gene DNA barcode region

Melo, Leonardo Barroso de; Alencar, Ronildo Baiatone; Scarpassa, Vera Margarete

doi:10.1016/j.meegid.2020.104256

Cited by 15 publications

(17 citation statements)

References 34 publications

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“…Curiously, molecular studies conducted with other sandflies from the Brazilian Amazon, such as L. anduzei 5 , B. flaviscutellata and Bichromomyia nociva 89 , 90 , which we presumed to have similar dispersion patterns, did not support this fluvial system acting as physical barriers between populations of these species. Such differences may suggest either lack of a common evolutionary history affecting these species or distinct dispersion rates for these species.…”

Section: Discussionmentioning

confidence: 70%

Multiple evolutionary lineages for the main vector of Leishmania guyanensis, Lutzomyia umbratilis (Diptera: Psychodidae), in the Brazilian Amazon

Scarpassa

Cunha-Machado

Alencar

2021

Sci Rep

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Lutzomyia umbratilis is the main vector of Leishmania guyanensis in the Brazilian Amazon and in neighboring countries. Previous biological and molecular investigations have revealed significant differences between L. umbratilis populations from the central Brazilian Amazon region. Here, a phylogeographic survey of L. umbratilis populations collected from nine localities in the Brazilian Amazon was conducted using two mitochondrial genes. Statistical analyses focused on population genetics, phylogenetic relationships and species delimitations. COI genetic diversity was very high, whereas Cytb diversity was moderate. COI genealogical haplotypes, population structure and phylogenetic analyses identified a deep genetic differentiation and three main genetic groups. Cytb showed a shallower genetic structure, two main haplogroups and poorly resolved phylogenetic trees. These findings, allied to absence of isolation by distance, support the hypothesis that the Amazon and Negro Rivers and interfluves are the main evolutionary forces driving L. umbratilis diversification. The main three genetic groups observed represent three evolutionary lineages, possibly species. The first lineage occurs north of the Amazon River and east of Negro River, where Le. guyanensis transmission is intense, implying that L. umbratilis is an important vector there. The second lineage is in the interfluve between north of Amazon River and west of Negro River, an area reported to be free of Le. guyanensis transmission. The third lineage, first recorded in this study, is in the interfluve between south of Amazonas River and west of Madeira River, and its involvement in the transmission of this parasite remains to be elucidated.

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Section: Discussionmentioning

confidence: 70%

Multiple evolutionary lineages for the main vector of Leishmania guyanensis, Lutzomyia umbratilis (Diptera: Psychodidae), in the Brazilian Amazon

Scarpassa

Cunha-Machado

Alencar

2021

Sci Rep

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“…Bichromomyia olmeca seems to be a species complex composed of at least four different lineages: B. o. nociva (reported in this study), and B. o. olmeca (mainly localized in Mexico), B. o. bicolor (reported in Pánama, Colombia, and Brazil), and B. flaviscutellata sensu stricto (de Melo et al 2020). The species complex is found throughout Central America to Brazil (Galati, 2019) (GBIF: DOI: https://doi.org/10.…”

Section: Brumptomyia Brumpti Has Collection Records Inmentioning

confidence: 58%

“…The species complex is found throughout Central America to Brazil (Galati, 2019) (GBIF: DOI: https://doi.org/10. 15468/dl.g77tbs, years: 1953and Table S1 in (Moo-Llanes et al 2013) and in some instances-as is the case in the Los Guácharos cave-, lineages have been found to coexist in the same geographic range (Fairchild and Theodor 1971;Escobar Vasco 1989;Romero Ricardo et al 2013;de Melo et al 2020;Santatana et al 2020). The identification of Colombian isolates of this species poses the need to revisit the geographic range of each species, with a special emphasis on areas of coexistence which might facilitate gene flow between vector species.…”

Section: Brumptomyia Brumpti Has Collection Records Inmentioning

confidence: 95%

Diversity of cave Phlebotomines (Diptera: Psychodidae) from a Colombian cave

Velazquez

Stuckert²,

Vivero

et al. 2022

Preprint

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Sandflies are vector species of Leishmania, among many other pathogens, with a global distribution and a variety of ecological niches. Previous samplings have found that karstic formations (i.e., caves and folds formed by the erosion of limestone) serve as a natural habitat to sandfly species. The majority of samplings of cave sandfly diversity have occurred in Brazil and to date none have studied the species composition in a cave in the Northern Andes. We collected sandflies in the Cave-Los Guacharos-, in the state of Antioquia, Colombia. The sampling was carried out during two consecutive nights in September 2019. CDC-type light traps were installed inside the cavern and in other surrounding karst systems (caves and folds). In total, we identified 18 species of sandfly from the cave and surrounding karst systems, including three new records for Colombia (Bichromomyia olmeca nociva, Brumptomyia brumpti, and Warileya leponti), and provide the first karstic reports for four other species (Lutzomyia gomezi, Lutzomyia hartmanni, Pintomyia ovallesi, and Psychodopygus panamensis). We then used the results of our survey and published literature to test two hypotheses. First, that sandfly diversity in Neotropical caves is richest nearer to the equator and second that there is a phylogenetic signal of karstic habitat use in sandflies. Counter to our predictions, we found no evidence that diversity follows a latitudinal gradient. Further, we find no evidence of a phylogenetic signal of karstic habitat use, instead finding that the use of caves likely evolved multiple times across several genera. Our results highlight the importance of a wide sampling to understand the natural habitat of sandflies and other disease vectors.

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“…neivai specimens were not analysed, (b) differences in nucleotide substitution rate between nuclear and mitochondrial genes, (c) the number of samples analysed, (d) the smaller geographical area they assessed or (e) a combination among them. Currently, de Melo et al 23 reported even lower interspecific values inside Bichromomyia flaviscutellata complex between Bichromomyia olmeca olmeca (Vargas and Díaz-Nájera) and B. olmeca bicolor (Fairchild and Theodor). These authors concluded that these closely-related species represent the same species, supporting the morphological data.…”

Section: Discussionmentioning

confidence: 94%

Phylogenetic relationships of closely-related phlebotomine sand flies (Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus

Moya

Pech-May

Quintana

et al. 2020

Mem. Inst. Oswaldo Cruz

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BACKGROUND The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details. OBJECTIVES We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment. METHODS The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex. FINDINGS The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species. MAIN CONCLUSIONS This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank.

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Molecular taxonomy and phylogenetic inferences of Bichromomyia flaviscutellata complex based on the COI gene DNA barcode region

Cited by 15 publications

References 34 publications

Multiple evolutionary lineages for the main vector of Leishmania guyanensis, Lutzomyia umbratilis (Diptera: Psychodidae), in the Brazilian Amazon

Multiple evolutionary lineages for the main vector of Leishmania guyanensis, Lutzomyia umbratilis (Diptera: Psychodidae), in the Brazilian Amazon

Diversity of cave Phlebotomines (Diptera: Psychodidae) from a Colombian cave

Phylogenetic relationships of closely-related phlebotomine sand flies (Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus

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