Molecular techniques for the identification of enteric viruses in marine waters

Muscillo, Michele; Aulicino, Francesca Anna; Patti, Anna Maria; Orsini, Patrizia; Volterra, L; Fara, Gaetano Maria

doi:10.1016/0043-1354(94)90113-9

Cited by 19 publications

(8 citation statements)

References 19 publications

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“…These could be due to temperature differences, rain events, (Muscillo et al 1994;Tani et al 1995;Patti et al 1996). But since reoviruses are more common in the animal population, the relationship between reoviruses and coliphages, or their use as predictors of other anthropogenic viruses, is not currently well defined.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and correlations between coliphages and anthropogenic viruses in the Massachusetts Bay using enrichment and ICC-nPCR

Ballester

Fontaine

Margolin

2005

Journal of Water and Health

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We evaluated a two-step enrichment procedure to detect coliphages and an integrated cell culture-nested polymerase chain reaction (ICC-nPCR) to detect human astrovirus, enteroviruses, rotavirus and adenovirus type 40 and 41 in marine water samples collected by the Massachusetts Water Resource Authority (MWRA). MWRA has been monitoring its receiving waters for coliphages, anthropogenic viruses and indicator bacteria in order to evaluate the impact of Boston's Deer Island Sewage Treatment Plant discharge. Coliphages and enteric viruses were originally assayed using single agar overlay and most probable number cell culture (MPN) methods, respectively. Reanalysis of these samples for enteric viruses by ICC-nPCR demonstrated that 46% were positive for at least one virus compared with 23% with the MPN method. Use of the enrichment method showed a 47% increase in the detection of male specific and somatic coliphages compared with the single agar overlay method. Correlations between the presence of coliphages, enteric viruses and indicator bacteria were based on proximity to the treatment plant discharge, seasonal variations and site levels. The presence of enteric viruses was significantly correlated to coliphages but not to indicator bacteria. Preliminary comparative results demonstrate that effective and efficient monitoring of anthropogenic contamination can be achieved using these more sensitive and specific techniques.

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Section: Discussionmentioning

confidence: 99%

Occurrence and correlations between coliphages and anthropogenic viruses in the Massachusetts Bay using enrichment and ICC-nPCR

Ballester

Fontaine

Margolin

2005

Journal of Water and Health

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“…Previous studies have recognized enteric viruses as the causative agents in many nonbacterial gastroenteritis cases and outbreaks [4][5][6]. Detection of enteric viruses in treated drinking water has also been reported [5,[7][8][9].…”

Section: Introductionmentioning

confidence: 93%

Deposition kinetics of bacteriophage MS2 to natural organic matter: Role of divalent cations

Pham

Mintz

Nguyen

2009

Journal of Colloid and Interface Science

128

119

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“…PEG precipitation has been a component of methods used to extract the enteroviruses (EV), poliovirus (PV), rotavirus (RV), hepatitis A virus (HAV) and norovirus (NoV) from water samples (Ali and Abdel-Dayem 2003;Colombet et al 2007;Huang et al 2000;Lewis and Metcalf 1988;Ramia and Sattar 1979;Shieh et al 2008;Yang and Xu 1993). The procedures have been extended to extractions from sewage and faecal samples as well as for monitoring salt and freshwater contamination with EV, PV, HAV, NoV, adenovirus (AdV) and bacteriophages (Hale et al 1996;Hovi et al 2001;La Rosa et al 2007;Li et al 1998;Muscillo et al 1994;Schwab et al 1995;Shieh et al 1995;van den Berg et al 2005). RV, HAV and NoV have also been concentrated from fruit and vegetable eluates using PEG (Baert et al 2008;Guevremont et al 2006;Kim et al 2008;Leggitt and Jaykus 2000).…”

Section: Sample Preparationmentioning

confidence: 99%

Analytical Methods for Food and Environmental Viruses

Mattison

Bidawid

2009

Food Environ Virol

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There are many challenges for developing and selecting methods to detect enteric viruses from food and environmental samples. Growth methods are rarely available and the viruses have a low infectious dose, so methods must be very sensitive as well as specific. This review discusses methods for sample preparation, detection and typing, outlining strengths and weaknesses for different protocols. Enteric viruses are very stable in the environment and the development of effective detection methods is an important step towards reducing contamination of foods and the environment.

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Molecular techniques for the identification of enteric viruses in marine waters

Cited by 19 publications

References 19 publications

Occurrence and correlations between coliphages and anthropogenic viruses in the Massachusetts Bay using enrichment and ICC-nPCR

Occurrence and correlations between coliphages and anthropogenic viruses in the Massachusetts Bay using enrichment and ICC-nPCR

Deposition kinetics of bacteriophage MS2 to natural organic matter: Role of divalent cations

Analytical Methods for Food and Environmental Viruses

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