Molecular toxicity mechanism of nanosilver

McShan, Danielle; Ray, Paresh Chandra; Yu, Hongtao

doi:10.1016/j.jfda.2014.01.010

Cited by 674 publications

(455 citation statements)

References 128 publications

(200 reference statements)

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“…Several articles have also been written to explain mechanistically the mode of action of silver nanoparticles [15][16][17][18][19]. However, most work focused on the empirical observations of effects that lead to the eradication of the target microbes, and very little rationalization on the general antimicrobial pathways was performed.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of silver nanoparticles: A surface science insight

2015

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Summary Silver nanoparticles constitute a very promising approach for the development of new antimicrobial systems. Nanoparticulate objects can bring significant improvements in the antibacterial activity of this element, through specific effect such as an adsorption at bacterial surfaces. However, the mechanism of action is essentially driven by the oxidative dissolution of the nanoparticles, as indicated by recent direct observations. The role of Ag + release in the action mechanism was also indirectly observed in numerous studies, and explains the sensitivity of the antimicrobial activity to the presence of some chemical species, notably halides and sulfides which form insoluble salts with Ag + . As such, surface properties of Ag nanoparticles have a crucial impact on their potency, as they influence both physical (aggregation, affinity for bacterial membrane, etc.) and chemical (dissolution, passivation, etc.) phenomena. Here, we review the main parameters that will affect the surface state of Ag NPs and their influence on antimicrobial efficacy. We also provide an analysis of several works on Ag NPs activity, observed through the scope of an oxidative Ag + release.

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Section: Introductionmentioning

confidence: 99%

“…Only general trends will thus be described. More information can be found in excellent reviews on the subject [15][16][17][18][19]. Additionally, toxicity issues for humans (systemic and at the cellular level) are topics of primary concern related to Ag NPs, but will not be developed here.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of silver nanoparticles: A surface science insight

2015

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“…The accumulation of ROS can cause perturbation, and destruction of the mitochondria. Proteins and enzymes with thiol groups within cells like glutathione, thioredoxin, SOD and thioredoxin peroxidase, are most important components of antioxidant defense mechanism, which is responsible for neutralizing the oxidative stress of ROS (Chen and Schluesener, 2008;McShan et al, 2014). However, reduced amounts of such proteins (SOD as an example of this group) and other groups of antioxidants (see results of FRAP assay) provide evidence that the antioxidant defense mechanism of Sertoli cells has been weakened by exposure to high levels of silver nanoparticles (125 ppm).…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Hassanpour

Mirshokraei

Sadrabad

et al. 2015

Animal

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To evaluate effects of different concentrations of nanosilver colloid on the cell culture of Sertoli cells, the proportion of lipid peroxidation, antioxidant capacity, nitric oxide (NO) production and genes expression of superoxide dismutases (SOD1 and SOD2) and nitric oxide synthases (eNOS and iNOS) were measured. Sertoli cells were incubated at concentrations of 25, 75 and 125 ppm nanosilver for 48 h. There was progressive lipid peroxidation in treatments according to increasing of nanosilver. Lipid peroxidation, as indicated by malondialdehyde levels, was significantly elevated by the highest concentration of silver colloid (125 ppm), although antioxidant capacity, as measured by ferric ion reduction, was unaffected. Nitrite, as an index of NO production was reduced only in 125 ppm of nanosilver. Expression of SOD1 gene was reduced in nanosilver-treated cells at all concentrations, whereas expression of SOD2 gene was reduced only in cells treated with 125 ppm nanosilver. Expression of iNOS gene was progressively increased with higher concentrations of nanosilver. Expression of eNOS gene was also increased in 125 ppm of nanosilver. In conclusion, toxic effects of nanosilver could be due to high lipid peroxidation and suppression of antioxidant mechanisms via reduced expression of SOD genes and increased expression of NOS genes.

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“…Among the metals, certainly the best-studied kind of material is nanosilver. While several in vitro studies could detect genotoxicity for various types of nanosilver, most in vivo studies failed to do so (reviewed in McShan et al 2014;Reidy et al 2013;Klien and Godnic-Cvar 2012). The role of nanosilver dissolution on this outcome is still not fully understood.…”

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confidence: 99%

“…However, particulate silver may also exert adverse effects. For instance, it was shown that nanosilver can directly interact with DNA, thereby altering the DNA structure (McShan et al 2014). By contrast, studies on other metal nanoparticles, except nanogold, are not as frequent.…”

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confidence: 99%