Molecular typing and antifungal susceptibility of Trichophyton rubrum isolates from patients with onychomycosis pre- and post-treatment

Santos, Danielle Anjos dos; Araújo, Roberta Amália de Carvalho; Kohler, Lidiane Meire; Machado‐Pinto, Jackson; Hamdan, Júnia Soares; Cisalpino, Patrícia Silva

doi:10.1016/j.ijantimicag.2006.09.028

Cited by 27 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 60 The minimal inhibitory concentration (MIC) of griseofulvin varied among strains (MICs of 0.062–1 μg ml –1 ) 88 . A study comparing the pre‐ and posttreatment isolates of T. rubrum found terbinafine to be the most potent agent, followed by itraconazole, clotrimazole, isoconazole, miconazole, cyclopiroxolamine, ketoconazole, griseofulvin and fluconazole 89 . However, posttreatment isolates showed an increase in the MIC for most of the azoles.…”

Section: Drug Resistancementioning

confidence: 99%

See 1 more Smart Citation

An update on treatment of onychomycosis

Grover

Khurana

2012

Mycoses

View full text Add to dashboard Cite

Onychomycosis (OM) is a fungal infection of the nail plate or nail bed which is highly prevalent in the general population and also responsible for significant morbidity. The condition needs to be treated in view of the physical and emotional handicap it produces. The peculiarities of the nail apparatus in health and disease lead to difficulties in being able to successfully treat this condition. Hence, the very same antifungals which produce high cure rates in skin infections are rendered less efficacious in nail disease. Low cure rates and high relapse rates even with highly efficacious antifungals have lead to an increasing interest in exploring newer treatment options which can ensure drug penetration, drug persistence, mycological cure and effective prevention of relapse. The current review aims to summarize our current status of knowledge about the treatment options for OM. It also summarizes the newer areas of research especially with respect to devices related therapies; physical measures to enhance penetration through nail; and development and evaluation of synergistic combinations.

show abstract

Section: Drug Resistancementioning

confidence: 99%

“…However, posttreatment isolates showed an increase in the MIC for most of the azoles. Interestingly, twelve patients exhibited different strains before and after the antifungal therapy 89 . Santos et al.…”

Section: Drug Resistancementioning

confidence: 99%

An update on treatment of onychomycosis

Grover

Khurana

2012

Mycoses

View full text Add to dashboard Cite

show abstract

“…It was speculated by the authors that the greater genetic diversity revealed in their study might result from having used strains exclusively from patients with onychomycosis. This condition, with its frequent chronicity, has been associated with mixed infections by multiple T. rubrum genotypes [19]. Collectively, the results of the studies cited above differ in terms of genetic polymorphism achieved by RAPD with both of the primers.…”

Section: Discussionmentioning

confidence: 99%

Identification and differentiation of Trichophyton rubrum clinical isolates using PCR-RFLP and RAPD methods

Hryncewicz-Gwóźdź

Jagielski

Dobrowolska

et al. 2011

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

Trichophyton rubrum represents the most frequently isolated causative agent of superficial dermatophyte infections. Several genotyping methods have recently been introduced to improve the delineation between pathogenic fungi at both the species and the strain levels. The purpose of this study was to apply selected DNA fingerprinting methods to the identification and strain discrimination of T. rubrum clinical isolates. Fifty-seven isolates from as many tinea patients were subjected to species identification by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and strain differentiation using a randomly amplified polymorphic DNA (RAPD) method, with two primers designated 1 and 6. Using PCR-RFLP, 55 of the isolates studied were confirmed to be T. rubrum. Among those, a total of 40 and five distinct profiles were obtained by RAPD with primers 1 and 6, respectively. The combination of profiles from both RAPD assays resulted in 47 genotypes and an overall genotypic diversity rate of 85.4%. A dendrogram analysis performed on the profiles generated by RAPD with primer 1 showed most of the isolates (87.3%) to be genetically related. PCR-RFLP serves as a rapid and reliable method for the identification of T. rubrum species, while the RAPD analysis is rather a disadvantageous tool for T. rubrum strain typing.

show abstract

“…1 Genetic strain typing methods have revolutionized epidemiological studies of dermatophyte infections as they are sensitive enough to distinguish a high number of DNA strain types left uncharacterized by morphological identification, which is limited by the cultural stability and differentiation of strains. 2 T. rubrum DNA strain types have been characterized using a number of methods, [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] but no correlation between DNA strain type and culture morphology has been detected, 10,14,19 even after multiple passages over the course of a year. 20 Geographical trends have been found where isolates from Europe have been distinguished from Japanese isolates, 16 African isolates distinguished from non-African isolates, 10,12 and Japanese isolates distinguished from Chinese isolates.…”

Section: What Does This Study Add?mentioning

confidence: 99%