Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals

Abstract

Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK efflux pump (32.14%) in the MDR isolates. FimH-1 and mrkD genes were prevalent in wound and blood isolates. FimH-1 gene was prevalent in sputum while mrkD gene was prevalent in urine. Serum resistance associated with outer membrane protein coding gene (traT) was found in all blood isolates. IucC, entB, and Irp-1 were detected in 32.14%, 78.5% and 10.7% of MDR isolates, respectively. We used two Polymerase Chain Reaction (PCR) analyses: Enterobacterial Repetitive Intergenic Consensus (ERIC) and Random Amplified Polymorphic DNA (RAPD). ERIC-PCR revealed 21 and RAPD-PCR revealed 18 distinct patterns of isolates with similarity ≥80%. ERIC genotyping significantly correlated with resistance patterns and virulence determinants. RAPD genotyping significantly correlated with resistance patterns but not with virulence determinants. Both RAPD and ERIC genotyping methods had no correlation with the capsule types. These findings can help up better predict MDR Klebsiella pneumoniae outbreaks associated with specific genotyping patterns.

Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

“…Virulence genes (magA, iucB, iroB, entB, irp1, iroN, kfuBC, rmpA, wcaG, alls, ybtA, ureA, uge, wabG, fimH and mrkD) and capsular serotypes (K1, K2, K5, K20, K54 and K57) of the six K. pneumoniae clinical isolates and K. pneumoniae ATCC 700603 were amplified by Polymerase Chain Reaction (PCR). Primers for the aforementioned genes are listed in Supplementary Table S1 [21,22]. Positive PCR products were sequenced by Beijing Genomics Institute Technology Co. Ltd. (Shanghai, China).…”

In vitro Susceptibility of Multidrug-Resistant Klebsiella pneumoniae Isolates from an Egyptian Tertiary Care Hospital to Tigecycline and Colistin

“…Concomitant with this finding, Shawky et al, (2015) from a comparable study conducted in Egypt validated that 86% of their investigated isolates were colistin-sensitive. Outstandingly, Wasfi et al, (2016) and Chiu et al, (2017) disclosed that all of their MDR K. pneumoniae isolates were sensitive to colistin. Nonetheless, Tawfick et al, (2016) mentioned that only 55.8 % of their MDR K. pneumoniae isolates collected from cancer patients at the Egyptian National Cancer Institute were colistin-sensitive.…”

Molecular Docking of Multidrug Resistant Klebsiella pneumoniae from River Water of Klang Valley, Malaysia

“…This is because K.pneumoniae not only able to colonize human skin as normal flora, it also has a persistent long survival rate in hospitals and environmental surface. Thus, transmission is relatively easy among patients in hospitals through hand contact with hospital care personnel [1][2][3] . To control the wide transmission rate and easy outbreak of K.pneumoniae, many antibiotics have been used as treatment.…”