Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010

Abstract

<jats:p>Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.</jats:p>

Comparative Analysis of Rapid Dengue Testing and ELISA for NS1 Antigen and IgM in Acute Dengue Infection

“…It has risen to epidemic proportions and is endemic to many areas, both urban and rural. In our study highest prevalence was seen in the age groups between 11-50 years and with male preponderance which is seen in other studies also (Gupta et al, 2006;Chakravarti et al, 2005;Sarkar et al, 2012). The sensitivity of the rapid ICT tests for NS1 Ag& IgM in our study was more than 90%, and specificity more than 98% when compared to ELISA.…”

An epidemiological study of fever outbreak in Aurangabad, Maharashtra, India

“…11,12 Sarkar A et al, however, reported maximum cases in the age group 0-10 years with female preponderance (Figure 1, 2). 13 All of the cases were reported during the Rainy seasons, in accordance with the reported patterns of dengue transmission in monsoon and post monsoon season. 14,8 Environmental conditions favoured an explanation of Aedes aegypti and dengue virus transmission.…”

A Brief Study on Recent Epidemiological Trend of Dengue in ATeriary Care Hospital In Kolkata

“…Findings of Gupta et al 12 andChakravarti and Kumaria 13 were similar to our studyi.e., maximum cases was in the age group (21-30 years) and in the finding that the male patients clearly outnumberingthe female patients. However Sarkaret al 14 reported that maximum cases were in the age group ≤10 years and there was female preponderance in their study. The majority of the cases were reported during the post-monsoon seasons (August to November) with a peak during October and November in all the four years (2012)(2013)(2014)(2015), in accordance with the established findings by P. Reiter.…”