Molecular typing of Enterobacter cloacae by pulsed-field gel electrophoresis of genomic restriction fragments

Haertl, Rolf

doi:10.1016/0195-6701(93)90101-5

Cited by 19 publications

(23 citation statements)

References 14 publications

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“…Ribotyping (5) and pulsed-field gel electrophoresis have been used for typing of E. cloacae, with high discriminatory potential and good reproducibility (18); however, they are labor-intensive and time-consuming (29). Another method reported to discriminate bacteria of different genera to the species level is amplified ribosomal DNA restriction analysis (32).…”

Section: Discussionmentioning

confidence: 99%

PCR Analyses of tRNA Intergenic Spacer, 16S-23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter- and Intraspecific Relationships of Enterobacter cloacae Strains

et al. 2001

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PCR analysis of tRNA intergenic spacer (tDNA-PCR) and of the 16S-23S internal transcribed spacer (ITS-PCR) and random amplified polymorphic DNA (RAPD) analysis were evaluated for their usefulness in characterization of Enterobacter cloacae strains isolated from both clinical origins and vaccine microbial contamination. tDNA-PCR presented specific and reproducible patterns for Enterobacter sakazakii ATCC 29004, Enterobacter aerogenes ATCC 13048, and Enterobacter cloacae ATCC 13047 and 23355 that presented the same profile for all 16 E. cloacae isolates, offering an alternative tool for species-level identification. ITS-PCR and RAPD analysis yielded completely different banding patterns for the 20 strains studied, except for E. cloacae strains isolated from different batches of vaccine that exhibited a unique pattern, suggesting contamination by the same strain. The combined use of tDNA-PCR and ITS-PCR in a one-step protocol allows accurate identification and typing of E. cloacae strains a few hours after the colony has been isolated.

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Section: Discussionmentioning

confidence: 99%

PCR Analyses of tRNA Intergenic Spacer, 16S-23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter- and Intraspecific Relationships of Enterobacter cloacae Strains

et al. 2001

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“…Genotyping was performed to evaluate the clonal relationship of the K. pneumoniae and E. cloacae isolates by pulsed-field gel electrophoresis (PFGE) (14), and K. pneumoniae isolates were additionally genotyped by multilocus sequence typing (MLST), as described previously (15). The two KPC-2-producing K. pneumoniae isolates were clonally related and belonged to the sequence type 307 (ST307).…”

mentioning

confidence: 99%

Spread of NDM-1-Producing Enterobacteriaceae in a Neonatal Intensive Care Unit in Istanbul, Turkey

Poirel

Yılmaz

Istanbullu

et al. 2014

Antimicrob Agents Chemother

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Twenty-two consecutive carbapenem-resistant enterobacterial isolates were recovered from patients hospitalized between January and April 2013 in different units at a university hospital in Istanbul, Turkey. These were Klebsiella pneumoniae isolates producing the carbapenemases OXA-48, NDM-1, and KPC-2, Enterobacter cloacae isolates producing NDM-1, and Escherichia coli isolates producing OXA-48. Most of the OXA-48-producing K. pneumoniae and all the NDM-1-producing E. cloacae were clonally related. The NDM-1-producing E. cloacae isolates recovered from a single neonatal intensive care unit corresponded to a single cluster, highlighting the spread of that clone in that setting.

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“…PFGE has been used to study the dissemination of ciprofloxacin-susceptible Enterobacter strains in detail in various clinical settings (14,27,(29)(30)(31). As such, the method is well accepted and has proven epidemiologic efficacy.…”

Section: Outbreaks Caused By Ciprofloxacin-resistant Microorganismsmentioning

confidence: 99%

Rapid Emergence of Ciprofloxacin-Resistant Enterobacteriaceae Containing Multiple Gentamicin Resistance-Associated Integrons, the Netherlands

Belkum¹,

Goessens²,

Schee³

et al. 2001

Emerg. Infect. Dis.

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In a hematology unit in the Netherlands, the incidence of ciprofloxacin-resistant Enterobacter cloacae and Escherichia coli increased from <0.5% to 20.7% and <0.5% to 64%, respectively, from 1996 to 1999. Clonal spread of single genotypes of both ciprofloxacin-resistant E. coli and Enterobacter cloacae from patient to patient was documented by pulsed-field gel electrophoresis and random amplification of polymorphic DNA. In addition, genetically heterogeneous strains were isolated regularly. Integrons associated with gentamicin resistance were detected in Enterobacter cloacae and E. coli strains. Integron-containing E. coli were detected in all hematology wards. In contrast, in Enterobacter cloacae strains two integron types were encountered only in the isolates from one ward. Although in all patients identical antibiotic regimens were used for selective decontamination, we documented clear differences with respect to the nosocomial emergence of ciprofloxacin-resistant bacterial strains and gentamicin resistance-associated integrons.

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Molecular typing of Enterobacter cloacae by pulsed-field gel electrophoresis of genomic restriction fragments

Cited by 19 publications

References 14 publications

PCR Analyses of tRNA Intergenic Spacer, 16S-23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter- and Intraspecific Relationships of Enterobacter cloacae Strains

PCR Analyses of tRNA Intergenic Spacer, 16S-23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter- and Intraspecific Relationships of Enterobacter cloacae Strains

Spread of NDM-1-Producing Enterobacteriaceae in a Neonatal Intensive Care Unit in Istanbul, Turkey

Rapid Emergence of Ciprofloxacin-Resistant Enterobacteriaceae Containing Multiple Gentamicin Resistance-Associated Integrons, the Netherlands

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