Molecular Typing of Rootstock Hybrids (Castanea Sativa X Castanea Crenata) and Portuguese Castanea Sativa Cultivars Based on Rapd Markers

Santana, Carlos; Oliveira, Cristina M.; Valdiviesso, T.

doi:10.17660/actahortic.1999.494.44

Cited by 10 publications

(3 citation statements)

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“…The results obtained by indicate that SSR markers provide a powerful tool for the fi ngerprinting exhibiting advantages over RAPD and AFLP. The RAPD markers, used in chestnut by Fineschi et al (1993), , Paffetti et al (1999), and Santana et al (1999), are very sensitive to the PCR conditions and therefore are less reproducible in different laboratories. AFLP, that were used by Yamamoto et al (1998) for the genetic characterization of Asian species of Castanea, are extremely polymorphic but are based on a complex technique that requires high DNA quality.…”

Section: Resultsmentioning

confidence: 99%

“…Germplasm collection and characterization has been carried out in Spain, France, Italy and Greece and genetic studies on both cultivated and wild populations have been conducted by several authors (Fineschi et al, 1993;Paffetti et al, 1999;Pereira et al, 1999;Pereira-Lorenzo and Fernandez-Lopez, 1993;Pereira-Lorenzo et al, 1996;Santana et al, 1999;Villani et al, 1991Villani et al, , 1993 using isozyme, randomly amplifi ed polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers, with the aim of assessing genetic variability, constructing phylogenetic trees and fi ngerprinting cultivars. In addition, linkage maps for Castanea have been constructed (Barreneche et al, 2003;Casasoli et al, 2001;Kubisiak et al, 1997).…”

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confidence: 99%

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Typing European Chestnut (Castanea sativa Mill.) Cultivars Using Oak Simple Sequence Repeat Markers

Boccacci¹,

Akkak²,

Marinoni³

et al. 2004

HortSci

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Microsatellite or simple sequence repeat (SSR) markers show many characteristics of the ideal molecular marker, and recent studies have shown that loci developed in one species may allow analysis in taxonomically related species. In this study, 52 primer pairs developed in two oak species—Quercus robur L. and Quercus petraea (Matt.) Lieb.—were used to amplify DNA of 5 chestnut cultivars; 28 of them yielded amplicons and 12 polymorphic loci were selected and used to fingerprint 12 european chestnut (Castanea sativa Mill.) cultivars grown in the Piedmont region of northwestern Italy. The number of alleles per locus ranged from 3 to 8, mean expected heterozygosity was 0.592 (range: 0.288 to 0.868), and mean observed heterozygosity was 0.667 (range: 0.333 to 1.000). The results demonstrate the usefulness of some SSR markers isolated in Quercus for the fingerprinting and genetic mapping of Castanea cultivars.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Typing European Chestnut (Castanea sativa Mill.) Cultivars Using Oak Simple Sequence Repeat Markers

Boccacci¹,

Akkak²,

Marinoni³

et al. 2004

HortSci

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“…1997b). Among molecular markers the RAPD markers are the most common (Fineschi et al 1993;Botta et al 1999;Seabra et al 1998;Santana et al 1999;Oraguize et al 1998;Galderisi et al 1998;Podjavor s sek 1999).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Diversity of European Chestnut (Castanea sativa Mill.) in Slovenia – Opportunity for Genetic Improvement

Solar

Podjavoršek²,

Štampar

2005

Genet Resour Crop Evol

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Phenotypic diversity of 244 chestnut trees was investigated. They originate from the Mediterranean and from two continental regions in Slovenia. In 3 years of analyses, length, diameter, thickness and weight of fruits; length and width of hilum, the pellicle intrusion; shape and colour of fruits; and the embryony were dealt with. The continental trees have smaller fruits than the Mediterranean trees; their fruits show greater variability in shape and the pellicle intrusion is stronger. They also exhibit polyembriony more frequently and they rarely have darker stripes. The sample of 46 trees from all three regions was used for the comparison between the phenotypic and genotypic diversity which had been investigated by RAPD analysis. In both cases, the UPGMA method was used for the classification of the trees into groups. Six pomological clusters were established. The clusters I, II, III and V comprise only the trees from the continental part. The cluster IV comprises nine trees of the marron type from the Mediterranean and one KOZ1 tree with equal pomological characteristics but originating from the continental part. The cluster VI is composed of eight trees from the continental part and one RAV3 tree whose fruits are very small and it originates from the Mediterranean. The Jaccard's coefficient of similarity is used in order to evaluate genetic relations. On the RAPD dendrogram four clusters of trees are defined. The continental trees are divided into three clusters and exhibit greater genotypic diversity than the Mediterranean trees which form only one cluster. With the RAPD analyses the differentiation of the trees with regard to their pomological traits is determined in 60-90% of the cases. The outcome of the research point to the fact that the Slovene chestnut is a rich source of genetic diversity and is very suitable for further breeding purposes.

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HPCE quantification of 5-methyl-2′-deoxycytidine in genomic DNA: Methodological optimization for chestnut and other woody species

Hasbún¹,

Valledor²,

Rodrı́guez³

et al. 2008

Plant Physiology and Biochemistry

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Molecular Typing of Rootstock Hybrids (Castanea Sativa X Castanea Crenata) and Portuguese Castanea Sativa Cultivars Based on Rapd Markers

Cited by 10 publications

References 0 publications

Typing European Chestnut (Castanea sativa Mill.) Cultivars Using Oak Simple Sequence Repeat Markers

Typing European Chestnut (Castanea sativa Mill.) Cultivars Using Oak Simple Sequence Repeat Markers

Phenotypic and Genotypic Diversity of European Chestnut (Castanea sativa Mill.) in Slovenia – Opportunity for Genetic Improvement

HPCE quantification of 5-methyl-2′-deoxycytidine in genomic DNA: Methodological optimization for chestnut and other woody species

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