Molecular variation and restriction fragment length polymorphisms (RFLPs) within and between six species of Verticillium

Carder, John; Barbara, D. J.

doi:10.1016/s0953-7562(09)80090-7

Cited by 66 publications

(43 citation statements)

References 20 publications

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“…albo-atrum’ [46], and have long been recognized as two genetically distinct groups [47], [48]. The two species are morphologically indistinguishable, but differ in pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Descriptions of Five New Species

et al. 2011

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Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management, and accurate identification of the pathogen is a foundation of pathogen biology. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticillium, a genus of ascomycete fungi, contains important plant pathogens whose species boundaries have been ill defined. Using phylogenetic analyses, morphological investigations and comparisons to herbarium material and the literature, we established a taxonomic framework for Verticillium comprising ten species, five of which are new to science. We used a collection of 74 isolates representing much of the diversity of Verticillium, and phylogenetic analyses based on the ribosomal internal transcribed spacer region (ITS), partial sequences of the protein coding genes actin (ACT), elongation factor 1-alpha (EF), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS). Combined analyses of the ACT, EF, GPD and TS datasets recognized two major groups within Verticillium, Clade Flavexudans and Clade Flavnonexudans, reflecting the respective production and absence of yellow hyphal pigments. Clade Flavexudans comprised V. albo-atrum and V. tricorpus as well as the new species V. zaregamsianum, V. isaacii and V. klebahnii, of which the latter two were morphologically indistinguishable from V. tricorpus but may differ in pathogenicity. Clade Flavnonexudans comprised V. nubilum, V. dahliae and V. longisporum, as well as the two new species V. alfalfae and V. nonalfalfae, which resembled the distantly related V. albo-atrum in morphology. Apart from the diploid hybrid V. longisporum, each of the ten species corresponded to a single clade in the phylogenetic tree comprising just one ex-type strain, thereby establishing a direct link to a name tied to a herbarium specimen. A morphology-based key is provided for identification to species or species groups.

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“…albo-atrum’ [46], and have long been recognized as two genetically distinct groups [47], [48]. The two species are morphologically indistinguishable, but differ in pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Descriptions of Five New Species

et al. 2011

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“…Using restriction fragment length polymorphism (RFLP) analysis, Typas et al (1992) reported that mitochondrial DNA of Verticillium species were distinctive and easily differentiated V. albo-atrum (from alfalfa) from other V. albo-atrum isolates. Carder and Barbara (1991) used RFLP analysis to differentiate V. dahliae from all isolates of V. albo-atrum and found intraspecific variation within V. dahliae isolates. Subsequently, Okoli et al (1993) probed Southern blots derived from 17 isolates of V. dahliae with 71 random genomic clones from V. dahliae and found that 15 isolates fitted clearly into two RFLP groups designated A and B.…”

Section: Species Identification and Numbersmentioning

confidence: 99%

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

et al. 2014

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Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry. They are also part of the natural ecosystem and may play a role in regulating plant numbers/density. Morphological identification and analysis of plant pathogenic fungi, while important, is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi. Molecular (DNA sequence) data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies, although hampered in part by non-standard laboratory practices and analytical methods. To facilitate current and future research, this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota, Basidiomycota, Mucormycotina (Fungi), and Oomycota, using recent molecular data, up-to-date names, and the latest taxonomic insights. Lineagespecific laboratory protocols together with advice on their application, as well as general observations, are also provided. We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge. Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort. Bipolaris, Botryosphaeriaceae,

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“…Thus, characterization and genetic differentiation of V. dahliae (and/or other Verticillium spp.) isolates has been carried out by restriction fragment length polymorphism (RFLP) (Carder and Barbara 1991;Typas et al 1992;Carder et al 1994;Okoli et al 1994;Dobinson et al 2000), random amplified polymorphic DNA (RAPD) (Messner et al 1996;Koike et al 1996;Zeise and von Tiedemann 2002;Bhat and Subbarao 1999), and amplified fragment length polymorphism (AFLP) (Collins et al 2003;Fahleson et al 2003;Radišek et al 2003;ColladoRomero et al 2006). Analysis and comparison of DNA sequences such as the intergenic spacer (IGS) and internal transcribed spacer (ITS) regions of genes encoding ribosomal RNA, as well as repeated DNA sequences, have also been used to study variation within V. dahliae (Morton et al 1995a, b;Subbarao et al 1995;Dobinson et al 1998;Pramateftaki et al 2000).…”

Section: Pathogenicity Groupsmentioning

confidence: 99%

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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Olive (Olea europaea L.) is one of the first domesticated and cultivated tree species and has historical, social and economical relevance. However, its future as a strategic commodity in Mediterranean agriculture is threatened by diverse biotic (traditional and new/emerging pests and diseases) and abiotic (erosion, climate change) menaces. These problems could also be of relevance for new geographical areas where olive cultivation is not traditional but is increasingly spreading (i.e., South America, Australia, etc). One of the major constraints for olive cultivation is Verticillium wilt, a vascular disease caused by the soil-borne fungus Verticillium dahliae Kleb. In this review we describe how Verticillium wilt of olive (VWO) has become a major problem for olive cultivation during the last two decades. Similar to other vascular diseases, VWO is difficult to manage and single control measure are mostly ineffective. Therefore, an integrated disease management strategy that fits modern sustainable agriculture criteria must be implemented. Multidisciplinary research efforts and advances to understand this pathosystem and to develop appropriate control measures are summarized. The main conclusion is that a holistic approach is the best strategy to effectively control VWO, integrating biological, chemical, physical, and cultural approaches.

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Molecular variation and restriction fragment length polymorphisms (RFLPs) within and between six species of Verticillium

Cited by 66 publications

References 20 publications

Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Descriptions of Five New Species

Phylogenetics and Taxonomy of the Fungal Vascular Wilt Pathogen Verticillium, with the Descriptions of Five New Species

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

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