Molecular variation in melon (Cucumis melo L.) as revealed by RFLP and RAPD markers

Silberstein, Leah; Kovalski, Irina; Huang, Ruguo; Anagnostou, Kostas; Jahn, Molly M.; Perl‐Treves, Rafael

doi:10.1016/s0304-4238(98)00199-x

Cited by 61 publications

(41 citation statements)

References 14 publications

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“…The level of polymorphism observed in the entire data set was found to be 90.90%, which were in more or less harmony with those of Velasquez and Gepts (1994), who observed 70% polymorphism in Phaseolus vulgaris. The results obtained further strengthened the previous findings of Nodari et al (1992) and Silberstein et al (1999). Gentzbittel et al (1992) also recorded a high level of polymorphism in Helianthus.…”

Section: Resultssupporting

confidence: 90%

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Islam¹,

Mir²,

Kudesia³

2015

International J. of Plant Breeding and Genetics

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Seed storage protein profiling through SDS-PAGE and DNA Restriction Fragment Length Polymorphism (RFLPs) were surveyed in 5 accessions of mungbean (Vigna radiata L.) sampled from NBPGR Thrissur, Kerala, India, to evaluate genetic diversity. The protein profile showed a low level of polymorphism (26.66%). The similarity index calculated ranged from (42.30-50%). The dendrogram constructed revealed two clusters. Cluster 1st comprises two accessions IC 251432 and IC 251433. Cluster 2nd comprises 3 cultivars viz., IC 251431, IC 251430 and IC 251434. Cluster 2nd also revealed a 100% homology between IC 251431 and IC 251430 accessions. The RFLP analysis was carried out by single restriction enzyme EcoRI. The RFLP profile revealed a high level of polymorphism (90.90%). A total of 11 clear bands were scored. The only band with fragment size 630 kb was monomorphic while, the rest were polymorphic. Similarity index ranged from (42.82-11.11%). The UPGMA analysis divided the accessions into 2 clusters viz., cluster 1st comprising 4 accessions (IC 251431, IC 251432, IC 251433 and IC 251430). Cluster 2nd includes only one accession (IC 251434) occupying a distinct position in constructed dendrogram.

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Section: Resultssupporting

confidence: 90%

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Islam¹,

Mir²,

Kudesia³

2015

International J. of Plant Breeding and Genetics

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“…The genetic difference between horticultural groups of melon has been studied by analysis of the isozyme (Kato et al 1998) and by using RAPD (Silberstein et al 1999, Stepansky et al 1999, Feyzian et al 2007, Sensoy et al 2007. However, these studies clustered together Inodorus, Cantalupensis and Flexuosus, and thus none of the studies succeeded in separating the three groups of largeseed type.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Iranian melon landraces of Cucumis melo L. Groups Flexuosus and Dudaim by analysis of morphological characters and random amplified polymorphic DNA

et al. 2010

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Diversity among Iranian melon landraces of Groups Flexuosus and Dudaim was studied by analyzing morphological and physiological traits and by random amplified polymorphic DNA (RAPD). Thirty-one morphological and physiological traits showed significant variation among accessions. Some Flexuosus accessions had typical morphological characters of elongated fruit shape, light skin color, ribs on fruit skin and nonsweet flesh. Characters distinct from in typical accessions, such as short fruits, dark green skin color, five carpels, sweet flesh, were especially in ribless accessions. Cluster analysis of morphological and physiological characters divided Iranian melon into seven groups. Dudaim (cluster VII) was clearly separated from Flexuosus, in which typical (cluster I) accessions and atypical accessions (clusters III-VI) were grouped separately. The diversity index shown by RAPD was 0.201 in 25 Flexuosus accessions and was rich in genetic diversity. Cluster analysis using RAPD divided Flexuosus accessions into eight subclusters, and clarified genetic similarity between Iranian melon accessions and reference accessions of large-seed type (Groups Inodorus and Cantalupensis). These results may suggest that large-seed Flexuosus, Inodorus and Cantalupensis are not differentiated genetically, probably due to spontaneous inter-group hybridization.

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“…But according to Stepansky, Kovalski, and Perl-Treves (1999a) and Silberstein et al (1999), Group Conomon and African melons (no distinct market classes) possess closer genetic affinities than are shared between Group Conomon and Groups Flexuosus and Inodorus. In fact, the hypothesized close genetic relationship between African landrace melons and Group Conomon Oriental market class melons was confirmed by Mliki et al (2001), suggesting a common origin.…”

Section: Cucumis Me/omentioning

confidence: 99%

“…Attempts to clarify interspecific taxonomic relationships within C. melo and evaluate its patterns of genetic diversity, assess market-dass relationships, and determine likely centers of origin for the various groupings have employed a diverse array of molecular markers, including isozymes (Esquinas-Alcazar 1981; Staub, Frederick, and Marty 1987;Akashi et al 2002b;McCreight et al 2004), RFLPs (Neuhausen 1992;Silberstein et al 1999), RAPDs Garcia et al 1998;Silberstein et al 1999;Stepansky, Kovalski, and Perl-Treves 1999a;Mliki et al 2001;L6pez-Sese et al 2002, L6pez-Sese, Staub, andGomez-Guillamon 2003), SSRs Danin-Poleg et al 2001;Decker-Walters et al 2002a;L6pez-Sese et al 2002;L6pez-Sese, Staub, and Gomez-Guillamon 2003;Monforte, Garcia Mas, and Ams 2003), ISSRs (Danin-Poleg et al l 998b;Perl-Treves et al 1998;Stepansky, Kovalski, and Perl-Treves 1999a), and AFLPs . Although both morphological and DNA (molecular-marker) variation has been used to define melon taxonomic groups and market classes, results derived from different lines of evidence do not always agree (L6pez-Sese, Staub, and Gomez-Guillamon 2003).…”

Section: Cucumis Me/omentioning

confidence: 99%

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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Molecular variation in melon (Cucumis melo L.) as revealed by RFLP and RAPD markers

Cited by 61 publications

References 14 publications

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Characterization of Iranian melon landraces of Cucumis melo L. Groups Flexuosus and Dudaim by analysis of morphological characters and random amplified polymorphic DNA

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

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