Molecular variation in populations of Erysiphe graminis on barley, oats and rye

O’Dell, M.; Wolfe, Martin S.; Flavell, R. B.; Simpson, Craig G.; Summers, R. W.

doi:10.1111/j.1365-3059.1989.tb02153.x

Cited by 38 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…la) indicates there are many repeated sequences in the genome ofthe species. A similar relatively high proportion of repeated DNA has been found in Erysiphe graminis (Day, 1986;O'Dell et al, 1989) and Fusarium (Coddington et al, 1987). A few genes are expected to be highly repeated, for example, those specifying the 18S, 25S and 5-8S ribosomal RNAs, as shown in this paper.…”

Section: Discussionsupporting

confidence: 81%

The classification of isolates of Gaeumannomyces graminis from wheat, rye and oats using restriction fragment length polymorphisms in families of repeated DNA sequences

O’Dell

Flavell

Hollins³

1992

Plant Pathology

Self Cite

View full text Add to dashboard Cite

Isolates of the take‐all fungus, Gaeumannomyces graminis var. avenae, which affects oats, wheat and other grasses, and of G.g. var. tritici, which preferentially affects wheat, rye and barley, contain a high proportion of repeated sequences. Total DNA from 57 fungal isolates collected from many locations and different cereal hosts, and scored for virulence on wheat, rye and oats, revealed many restriction fragment length polymorphisms. These RFLPs were observed either by staining the DNA directly, by hybridization to radioactively labelled total fungal DNA, or by hybridization with labelled wheat ribosomal DNA. With only a few exceptions, the isolates with the same preferred cereal hosts showed more similar patterns of restriction fragments than isolates that had different pathogenicity properties on cereal hosts, irrespective of the geographical origins of the isolates. This was even the case for R isolates of G.g. var. tritici that were virulent on wheat and rye compared with N isolates that were virulent only on wheat. Isolates were identified by hybridizing DNA from infected root samples with 32P‐labelled total fungal DNA. The restriction fragment polymorphisms involving families of repeated sequence can therefore be used as a predictive assay for host preference of an isolate, and have probably arisen by host selection of fungal lineages. The variation between isolates in different pathogenicity groups suggests that there is little gene flow between isolates that can infect different hosts, even though they can coexist in the same field.

show abstract

Section: Discussionsupporting

confidence: 81%

The classification of isolates of Gaeumannomyces graminis from wheat, rye and oats using restriction fragment length polymorphisms in families of repeated DNA sequences

O’Dell

Flavell

Hollins³

1992

Plant Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…g. f. sp. hordei genomic DNA, designated E9, has been isolated at the Cambridge Laboratory and shown to detect RFLPs when DNA extracts of different individuals, digested by Hindlll, are hybridized with E9 DNA (O'Dell et al, 1989). E9 does not hybridize to barley DNA; this allows isolates of E.g.…”

Section: Introductionmentioning

confidence: 99%

“…f.sp. hordei to be extracted together (O'Dell et al, 1989). The biological function, if any, of E9 is unknown.…”

Section: Introductionmentioning

confidence: 99%

The use of DNA polymorphisms to test hypotheses about a population of Erysiphe graminis f.sp. hordei

Brown¹,

O’Dell

Simpson

et al. 1990

Plant Pathology

Self Cite

View full text Add to dashboard Cite

The distribution of restriction‐fragment‐length polymorphisms (RFLPs) among isolates of Erysiphe graminis f.sp. hordei was used to test hypotheses about the structure of the pathogen population. There was a large diversity in the lengths of DNA restriction fragments homologous to E9, a chromosomal fragment of unknown function. It was shown that a large group of isolates, which shared identical or very similar virulence phenotypes, including virulence on the barley cv. Triumph, were members of a single clone. Several other clones were identified, including a second group of identical isolates which were virulent on cv. Triumph but were highly distinct from the more common clone of Triumph‐virulent isolates. Isolates which shared the same level of resistance to the triazole fungicide triadimenol were genetically diverse. Such diversity is consistent with triadimenol resistance being under oligogenic, rather than polygenic, control. Conclusions obtained from analysis of the most easily identifiable subset of fragment lengths were very similar to those obtained from analysis of the complete set.

show abstract

“…graminis possesses a high amount of characteristic isozyme and protein bands (cf. CLARK et al 1989), but also of many families of dispersed repeated DNA-sequences in each formae specialis (O'DELL et al 1989). The only exception is the high accordance between E. graminis ff.…”

Section: Discussionmentioning

confidence: 99%

Isozyme Variation and Genetic Distances of Erysiphe graminis DC. Formae Speciales

Koch

Köhler

1990

Journal of Phytopathology

View full text Add to dashboard Cite

Isozymes of ten different enzymes and unspecific stained proteins were used as biochemicalgenetic markers to study genetic variation wuhin and between E. graminis ff. sp. hordci, avenae, secalis and tritici. In addition, grain proteins of the corresponding host species were examined. In each forma specialis, one genotype proved to be predominant. 131 distinct isozyme and 93 protein bands were distinguishable in these genotypes. However, divergent banding patterns differed only in 8 bands from the predominant banding patterns found within the formae speciales avetta, secalis and tritici. The genetic relationships between powdery mildew formae speciales and host species were computed by cluster analysis from similarity (F) and dissimilarity (D) coefficients and illustrated by phylogenetic trees. Marked correspondence was found between E. graminis ff. sp. secalis and tritici {¥: 83-90%

show abstract

Molecular variation in populations of Erysiphe graminis on barley, oats and rye

Cited by 38 publications

References 13 publications

The classification of isolates of Gaeumannomyces graminis from wheat, rye and oats using restriction fragment length polymorphisms in families of repeated DNA sequences

The classification of isolates of Gaeumannomyces graminis from wheat, rye and oats using restriction fragment length polymorphisms in families of repeated DNA sequences

The use of DNA polymorphisms to test hypotheses about a population of Erysiphe graminis f.sp. hordei

Isozyme Variation and Genetic Distances of Erysiphe graminis DC. Formae Speciales

Contact Info

Product

Resources

About