Molecularly endowed hydrogel with an<i>in silico</i>-assisted screened peptide for highly sensitive small molecule harvesting

Natale, Concetta Di; Celetti, Giorgia; Scognamiglio, Pasqualina Liana; Cosenza, Chiara; Battista, Edmondo; Causa, Filippo; Netti, Paolo A.

doi:10.1039/c8cc04943b

Cited by 19 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD spectra were registered at 25 °C in the far UV region from 260 to 190 nm [ 29 ]. The spectra were obtained with an average of 3 scans by subtracting them from blank samples ( Figure S2 ) [ 30 , 31 , 32 , 33 , 34 , 35 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

Cell Membrane-Coated Oil in Water Nano-Emulsions as Biomimetic Nanocarriers for Lipophilic Compounds Conveyance

et al. 2021

Self Cite

View full text Add to dashboard Cite

Recently, we developed ultra-stable oil in water nano-emulsions (O/W NEs), able to carry both internal and external cargos (Somes), such as lipophilic compounds and hydrophilic coatings, respectively, that we call here NEsoSomes. O/W NEs are an excellent bioengineering tool for drug and molecules delivery, due to their ability to dissolve a large number of hydrophobic compounds and protect them from hydrolysis and degradation under biological conditions. At present, no report is available on the combination of cell membrane coatings with such nanocarriers, probably due to their typical instability feature. Since then, we have reported, for the first time, a new cell membrane (CM)-coated nanomaterial composed of membranes extracted from glioblastoma cancer cells (U87-MG) deposited on NEsoSomes, through a liquid–liquid interface method, to produce highly controllable membrane caked nano-capsules, namely CM-NEsoSomes. CM-NEsoSomes were physically characterized by dynamic light scattering (DLS) over time and their correct morphology was analyzed by confocal and transmission electron microscopy (TEM) microscopy. Moreover, CM-NEsoSomes biocompatibility was tested on the healthy model cell line, performing cell cytotoxicity and uptake assay, showing nanocarriers uptake by cells with no induced cytotoxicity.

show abstract

Section: Methodsmentioning

confidence: 99%

Cell Membrane-Coated Oil in Water Nano-Emulsions as Biomimetic Nanocarriers for Lipophilic Compounds Conveyance

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The external and internal morphology of the MPs was studied by SEM microscopy, as already described. 16,56 In detail, samples were attached onto a standard SEM pin stub, placed into a sputter coater which covered them with a 15 nm thickness gold layer and analyzed by FESEM at 15-25 kV using an SE2 detector. 100 mL of MPs were deposited on a standard SEM pin stub, gold-sputtered (10 nm thickness) using a HR208 Cressington sputter coater and analyzed by FESEM ULTRA-PLUS (Zeiss) at 5 kV with the SE2 detector.…”

Section: Fabrication Of Master and Micromoldmentioning

confidence: 99%

Design of biodegradable bi-compartmental microneedles for the stabilization and the controlled release of the labile molecule collagenase for skin healthcare

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…To obtain the production yields of each formulation, MPs suspensions were filtered and then lyophilized overnight (−50 • C, 0.73 hPa, Heto PowerDry PL6000 Freeze Dryer; Thermo Electron Corp., United States). The production (Celetti et al, 2016;Di Natale et al, 2018;Battisti et al, 2019;Jamaledin et al, 2020). Together with the average diameter (d50), for each size distribution, the SPAN value has also been evaluated, which is the distribution width calculated as:…”

Section: Alginate Mps Preparationmentioning

confidence: 99%

Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres

Natale

Lagreca

Panzetta

et al. 2021

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

The intestinal microbiota is a real ecosystem composed of several bacterial species and a very huge amount of strains that through their metabolic activities play a crucial role in the development and performance of the immune system and other functions. Microbiota modulation by probiotics establishes a new era into the pharmaceutical and healthcare market. Probiotics play, in fact, an important role in helping and sustaining human health, but in order to produce benefits, their viability must be preserved throughout the production process up to consumption, and in addition, their bioactivity required to be safeguarded while passing through the gastrointestinal tract. In this frame, encouraging results come from encapsulation strategies that have proven to be very promising in protecting bacteria and their viability. However, specific effort has to be dedicated to the design optimization of the encapsulation process and, in particular, to the processing parameters that affect capsules microstructure. Herein, focusing on calcium alginate microspheres, after a preliminary selection of their processing conditions based on size distribution, we implemented a micro-rheological analysis, by using the multiple-particle tracking technique, to correlate the inner microstructure to the selected process conditions and to the viability of the Lactobacillus paracasei CBA L74. It was assessed that the explored levels of cross-linking, although changing the microorganism constriction, did not affect its viability. The obtained results confirm how this technology is a promising and a valid strategy to protect the microorganism viability and ensure its stability during the production process.

show abstract

Molecularly endowed hydrogel with anin silico-assisted screened peptide for highly sensitive small molecule harvesting

Cited by 19 publications

References 34 publications

Cell Membrane-Coated Oil in Water Nano-Emulsions as Biomimetic Nanocarriers for Lipophilic Compounds Conveyance

Cell Membrane-Coated Oil in Water Nano-Emulsions as Biomimetic Nanocarriers for Lipophilic Compounds Conveyance

Design of biodegradable bi-compartmental microneedles for the stabilization and the controlled release of the labile molecule collagenase for skin healthcare

Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres

Contact Info

Product

Resources

About