Molecularly imprinted biocompatible magnetic nanoparticles for specific recognition of Ochratoxin A

Turan, Eylem; Şahin, Ferat

doi:10.1016/j.snb.2015.12.087

Cited by 59 publications

(25 citation statements)

References 52 publications

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“…Additionally, sensing of the toxin was reported to be possible from 0.374 µg mL −1 . Selectivity of the IP was tested using aflatoxin and vomitoxin as competitive mycotoxins, for which they observed lower adsorption capacities in comparison to the targeted ochratoxin A [187]. The results in Table 4 are in line with the findings described in Section 3, which makes sense as toxins are typically low-molecular weight molecules similar to drugs, pesticides or hormone disruptors.…”

Section: Toxinssupporting

confidence: 75%

Imprinted Polymers as Synthetic Receptors in Sensors for Food Safety

et al. 2021

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Foodborne illnesses represent high costs worldwide in terms of medical care and productivity. To ensure safety along the food chain, technologies that help to monitor and improve food preservation have emerged in a multidisciplinary context. These technologies focus on the detection and/or removal of either biological (e.g., bacteria, virus, etc.) or chemical (e.g., drugs and pesticides) safety hazards. Imprinted polymers are synthetic receptors able of recognizing both chemical and biological contaminants. While numerous reviews have focused on the use of these robust materials in extraction and separation applications, little bibliography summarizes the research that has been performed on their coupling to sensing platforms for food safety. The aim of this work is therefore to fill this gap and highlight the multidisciplinary aspects involved in the application of imprinting technology in the whole value chain ranging from IP preparation to integrated sensor systems for the specific recognition and quantification of chemical and microbiological contaminants in food samples.

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Section: Toxinssupporting

confidence: 75%

Imprinted Polymers as Synthetic Receptors in Sensors for Food Safety

et al. 2021

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“…[11] The production of MIPs to OTA has been until now based on bulk polymerization using OTA template or its structural analogues. [12] Unfortunately this approach, despite being simple, presents difficulties during the purification of MIPs due to the residual template left in polymeric matrix. In this research, we have used for the first time molecularly imprinted polymer nanoparticles (nanoMIPs) designed for OTA recognition.…”

Section: Introductionmentioning

confidence: 99%

Study of Epitope Imprinting for Small Templates: Preparation of NanoMIPs for Ochratoxin A

et al. 2019

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Ochratoxin A (OTA) is a carcinogenic mycotoxin produced by Aspergillus and Penicillium, which contaminates cereals like corn and wheat, in addition to beverages such as coffee, wine and beer. Chromatography is the preferred analytical method for OTA determination, but unfortunately this method is still elaborate, expensive and tedious. Alternative mycotoxin detection techniques involve assays and sensors based on molecularly imprinted polymers nanoparticles (nanoMIPs) as synthetic polymeric receptors mimicking antibodies. Herein we designed nanoMIPs for specific recognition of OTA using computational screening and docking of different acryl functional monomers. Subsequently, different nanoMIPs were prepared by solid phase synthesis using two OTA haptens, OTAe‐MIPs without carboxylic group and OTAb‐MIPs bearing the carboxylic group. NanoMIPs affinity towards OTA was analyzed by surface plasmon resonance, the results displayed dissociation constants for OTAb‐MIPs at 0.4 μM and for OTAe‐MIPs at 5.1 μM. Therefore, the results demonstrate that nanoMIPs recognition depends on the immobilization position of the OTA hapten as observed using antibodies.

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“…The diameter and shape of modified nanoparticles did not significantly change. It was due to the electron transparent small organic molecule TMSPM, did not show up in TEM …”

Section: Resultsmentioning

confidence: 99%

“…Molecularly imprinting, which has advantages such as good stability, cost‐effective, fast responsibility, is a great significant method to recognize and separate, selectively of template molecules with high affinity . Small molecules, toxins, proteins, etc.…”

Section: Introductionmentioning

confidence: 99%

His‐Tag‐Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples

Turan

2018

ChemistrySelect

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In the epitope imprinting method, a short peptide, even without a defined tertiary structure, can be used as the template to produce protein-selective polymers. In this study, His-tag-epitope imprinted magnetic nanoparticles (EMIP-MNPs) were prepared via epitope imprinting method onto the surface of the magnetic nanoparticles for specific recognition and separation of thyroid peroxidase antigens (TPO). 3-(Trimethoxysilyl) propyl methacrylate (TMSPM), copper (II) acrylate (Cu(II) Ac) and N-isopropylacrylamide (NIPAAm) were chosen as func-tional monomers. Ethyleneglycoldimethacrylate (EGDMA) and 2,2'-azobisisobutyronitrile (AIBN) were selected as crosslinking agent and initiator, respectively. The EMIP-MNPs were prepared within 30 min by epitope imprinting method which was shorter than the surface imprinting method. The prepared EMIP-MNPs were thermosensitive and showed selective affinity to target TPO antigens. Moreover, the EMIP-MNPs were easily and quickly recognized and separated to target antigens from the whole blood sample.

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Molecularly imprinted biocompatible magnetic nanoparticles for specific recognition of Ochratoxin A

Cited by 59 publications

References 52 publications

Imprinted Polymers as Synthetic Receptors in Sensors for Food Safety

Imprinted Polymers as Synthetic Receptors in Sensors for Food Safety

Study of Epitope Imprinting for Small Templates: Preparation of NanoMIPs for Ochratoxin A

His‐Tag‐Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples

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