Molecularly imprinted composite cryogels for hemoglobin depletion from human blood

Baydemir, Gözde; Andaç, Müge; Perçin, Işık; Derazshamshir, Ali; Deni̇zli̇, Adil

doi:10.1002/jmr.2376

Cited by 36 publications

(17 citation statements)

References 52 publications

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“…After depleted hemoglobin, they used electrophoresis system to show purified hemoglobin (Figure 7). Lanes 1, 2, 3 and 4 depict hemolysate before adsorption, protein ladder, desorbed hemoglobin and hemolysate after adsorption [53].…”

Section: Composite Cryogels As Versatile Tools For Biomedical Applmentioning

confidence: 99%

Supermacroporous Composite Cryogels in Biomedical Applications

Saylan

Denizli

2019

Gels

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Supermacroporous gels, called cryogels, are unique scaffolds that can be prepared by polymerization of monomer solution under sub-zero temperatures. They are widely used in many applications and have significant potential biomaterials, especially for biomedical applications due to their inherent interconnected supermacroporous structures and easy formation of composite polymers in comparison to other porous polymer synthesis techniques. This review highlights the fundamentals of supermacroporous cryogels and composite cryogels, and then comprehensively summarizes recent studies in preparation, functionalization, and utilization with mechanical, biological and physicochemical features, according to the biomedical applications. Furthermore, conclusions and outlooks are discussed for the use of these promising and durable supermacroporous composite cryogels.

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Section: Composite Cryogels As Versatile Tools For Biomedical Applmentioning

confidence: 99%

Supermacroporous Composite Cryogels in Biomedical Applications

Saylan

Denizli

2019

Gels

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“…Finally, a MIP‐cryogel composite membrane was fabricated via free radical polymerization at subzero temperature and applied in selective isolation of antibody from human plasma by protein liquid chromatography. Similarly, both BSA‐imprinted poly(GMA) and hemoglobin‐imprinted poly(HEMA‐ co ‐ N ‐methacryloyl‐ l ‐histidine) beads were synthesized by suspension polymerization and then integrated into MICs for removing albumin and hemoglobin from human serum, respectively. In the second approach, MICs were directly synthesized with N ‐methacryloyl‐ l ‐histidinemethylester (MAH) as the monomer via cryopolymerization, in which Cu 2+ chelates with MAH.…”

Section: Basic Strategy For Designing Monolithic Materialsmentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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“…A brief synopsis of the different types of protein and biomacromolecular surface‐imprinted monoliths and particles/beads is shown in Tables and , respectively, including the various surface imprinting strategies and characterization techniques that have been utilized.…”

Section: Imprinting Of Peptides and Proteins For The Use In Solid‐phamentioning

confidence: 99%

Advances in the development of molecularly imprinted polymers for the separation and analysis of proteins with liquid chromatography

Boysen

2018

J of Separation Science

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This review documents recent advances in the design, synthesis, characterization, and application of molecularly imprinted polymers in the form of monoliths and particles/beads for the use in the separation and analysis of proteins with solid‐phase extraction or liquid chromatography. The merits of three‐dimensional molecular imprinting, whereby the molecular template is randomly embedded in the polymer, and two‐dimensional imprinting, in which the template is confined to the surface, are described. Target protein binding can be achieved by either using the entire protein as a template or by using a protein substructure as template, that is, a peptide, as in the "epitope" approach. The intended approach and strategy then determine the choice of polymerization method. A synopsis has been provided on methods used for the physical, chemical, and functional characterizations and associated performance evaluations of molecularly imprinted and nonimprinted control polymers, involving a diverse range of analytical techniques commonly used for low and high molecular mass analytes. Examples of recent applications demonstrate that, due to the versatility of imprinting methods, molecularly imprinted monoliths or particles/beads can be adapted to protein extraction/depletion and separation procedures relevant to, for example, protein biomarker detection and quantification in biomedical diagnostics and targeted proteomics.

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Molecularly imprinted composite cryogels for hemoglobin depletion from human blood

Cited by 36 publications

References 52 publications

Supermacroporous Composite Cryogels in Biomedical Applications

Supermacroporous Composite Cryogels in Biomedical Applications

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Advances in the development of molecularly imprinted polymers for the separation and analysis of proteins with liquid chromatography

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