Molecularly imprinted polymer-liquid chromatography/fluorescence for the selective clean-up of hydroxylated polycyclic aromatic hydrocarbons in soils

Abstract

International audienceHydroxylated polycyclic arom. hydrocarbons (OH-PAHs), a group of metabolites produced during the biodegrdn. of PAHs by microorganisms, are known to be difficult to analyze from complex environmental or biol. matrixes. In the field of contaminated soils, where OH-PAHs are less studied, a new method was compared to a common method for the anal. of monohydroxylated PAH metabolites: 2-naphthol, 2-hydroxyfluorene, 9-phenanthrol and 1-hydroxypyrene. After optimizing the microwave assisted extn. (MAE) step, the obtained exts., contg. the OH-PAHs together with the corresponding PAHs, were purified by molecularly imprinted polymers (MIPs). MIPs showed an effective selectivity for the hydroxylated derivs. studied (and not for native PAHs), which allowed their identification and quantification by HPLC/FLD. By this method, good limits of detection (0.003-0.014 μg g-1) and limits of quantification (0.010-0.044 μg g-1) were achieved. On the other hand, following the common method, the sample ext. was concd. by evapn., silylated, and analyzed by GC/MS. The new method was more sensitive for all OH-PAHs and the anal. showed fewer matrix effects due to the use of MIP clean-up. In the present work the advantages and disadvantages of the new method are shown, as well as the possible application to soil biodegrdn. studies

Simultaneous separation of chlorinated/brominated dioxins, polychlorinated biphenyls, polybrominated diphenyl ethers and their methoxylated derivatives from hydroxylated analogues on molecularly imprinted polymers prior to gas/liquid chromatography and mass spectrometry

“…The compounds include mycotoxins [37], several EDC groups [38], phenolic compounds [39]. However, no report on application of MIPs to separate OH-CB/BDEs is available.…”

Influence of a mixture of metals on PAHs biodegradation processes in soils

“…benzo[a]pyrene (BaP) (five rings). The concentration of PAHs which was spiked into microcosms was selected according to the previously PAH content found in soil, following other related biodegradation studies (Baltrons et al, 2013;Niepceron et al, 2013) where soils were typically spiked at a concentration of 100-10,000 times to that found in soil.…”

Molecularly Imprinted Polymers (MIPs) in Sensors for Environmental and Biomedical Applications: A Review

“…MIPs have been fabricated for solid phase extraction [ 14 , 15 , 16 , 17 , 18 ], chromatographic separation [ 19 , 20 , 21 , 22 , 23 ], catalysis [ 24 , 25 , 26 , 27 , 28 ], drug delivery [ 29 , 30 , 31 , 32 , 33 ], study of the structure and function of proteins [ 34 , 35 , 36 , 37 , 38 ], environmental and biomedical sensing [ 39 , 40 , 41 , 42 , 43 ], water and wastewater treatment [ 44 , 45 , 46 , 47 , 48 ], and membrane-based separations [ 49 , 50 , 51 , 52 , 53 ]. MIP use for purification purposes is the most commercially available application, particularly in analytical chemistry; other uses are still in need of further development [ 54 ].…”