Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

Mustafa, Yasemin L.; Keirouz, Antonios; Leese, Hannah S.

doi:10.1039/d2tb00703g

Cited by 49 publications

(41 citation statements)

References 284 publications

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“…The microneedles themselves may be functionalized to elicit an electrical response to a change in the concentration of a target analyte, for example, by applying methylene blue modified aptamers for luteinizing hormone sensing or hydrogel enmeshed enzymes for glucose or penicillin sensing. − Aptamers may be bound to the surface of the microneedles and can be functionalized with a redox-active molecule that will be held closer or further from the electrode surface in the presence of the analyte. Molecularly imprinted polymers are also a possible recognition element that may be grown onto a microneedle surface . The majority of published work currently uses enzymes, however.…”

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confidence: 99%

Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes

et al. 2023

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Microneedle lactate sensors may be used to continuously measure lactate concentration in the interstitial fluid in a minimally invasive and pain-free manner. First- and second-generation enzymatic sensors produce a redox-active product that is electrochemically sensed at the electrode surface. Direct electron transfer enzymes produce electrons directly as the product of enzymatic action; in this study, a direct electron transfer enzyme specific to lactate has been immobilized onto a microneedle surface to create lactate-sensing devices that function at low applied voltages (0.2 V). These devices have been validated in a small study of human volunteers; lactate concentrations were raised and lowered through physical exercise and subsequent rest. Lactazyme microneedle devices show good agreement with concurrently obtained and analyzed serum lactate levels.

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confidence: 99%

Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes

et al. 2023

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“…The MIP sensors field has rapidly grown for the last ten years. Recently, many review articles describing the application of MIP sensors in several fields, namely, clinical diagnosis [51], cancer biomarkers detection [52,53], forensic sciences [54], warfareagents detection [55], and environmental analysis [51,56,57], were published. This rapid growth highlights the possibility of introducing MIP chemosensors to the market and applying them in everyday practice, i.e., food quality and safety control.…”

Section: Future Perspectives and Unsolved Challengesmentioning

confidence: 99%

Molecularly imprinted polymer-based electrochemical sensors for food contaminants determination

Ayerdurai

Cieplak

Kutner

2023

TrAC Trends in Analytical Chemistry

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“…MIPs in diagnostics have been recently reviewed that allow high-affinity analyte detection in various biological fluids, namely serum, saliva, cerebrospinal fluid, sweat, urine, nasopharyngeal fluid, and tears [ 48 ]. Our previous work also demonstrated the measurements of many biomarkers with MIPs in urine [ 45 ], saliva [ 49 ], and serum [ 50 ].…”

Section: Chemosensors With Molecularly Imprinted Polymersmentioning

confidence: 99%

The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions

et al. 2023

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The application of lab-on-a-chip technologies in in vitro cell culturing swiftly resulted in improved models of human organs compared to static culture insert-based ones. These chip devices provide controlled cell culture environments to mimic physiological functions and properties. Models of the blood-brain barrier (BBB) especially profited from this advanced technological approach. The BBB represents the tightest endothelial barrier within the vasculature with high electric resistance and low passive permeability, providing a controlled interface between the circulation and the brain. The multi-cell type dynamic BBB-on-chip models are in demand in several fields as alternatives to expensive animal studies or static culture inserts methods. Their combination with integrated biosensors provides real-time and noninvasive monitoring of the integrity of the BBB and of the presence and concentration of agents contributing to the physiological and metabolic functions and pathologies. In this review, we describe built-in sensors to characterize BBB models via quasi-direct current and electrical impedance measurements, as well as the different types of biosensors for the detection of metabolites, drugs, or toxic agents. We also give an outlook on the future of the field, with potential combinations of existing methods and possible improvements of current techniques.

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Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

Abstract: Bio-applied molecularly imprinted polymers (MIPs) are biomimetic materials with tailor-made synthetic recognition sites, mimicking biological counterparts known for their sensitive and selective analyte detection. MIPs, specifically designed for biomarker analysis...

Cited by 49 publications

References 284 publications

Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes

Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes

Molecularly imprinted polymer-based electrochemical sensors for food contaminants determination

The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions

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