Molecularly Imprinted Silica-Coated CdTe Quantum Dots for Fluorometric Determination of Trace Chloramphenicol

Abstract

A dual recognition system with a fluorescence quenching of quantum dots (QDs) and specific recognition of molecularly imprinted polymer (MIP) for the detection of chloramphenicol (CAP) was constructed. MIP@SiO2@QDs was prepared by reverse microemulsion method with 3-aminopropyltriethoxysilane (APTS), tetraethyl orthosilicate (TEOS) and QDs being used as the functional monomer, cross-linker and signal sources, respectively. MIP can specifically recognize CAP, and the fluorescence of QDs can be quenched by CAP due to the photo-induced electron transfer reaction between CAP and QDs. Thus, a method for the trace detection of CAP based on MIP@SiO2@QDs fluorescence quenching was established. The fluorescence quenching efficiency of MIP@SiO2@QDs displayed a desirable linear response to the concentration of CAP in the range of 1.00~4.00 × 102 μmol × L−1, and the limit of detection was 0.35 μmol × L−1 (3σ, n = 9). Importantly, MIP@SiO2@QDs presented good detection selectivity owing to specific recognition for CAP, and was successfully applied to quantify CAP in lake water with the recovery ranging 102.0~104.0%, suggesting this method has the promising potential for the on-site detection of CAP in environmental waters.

Simultaneous Dual-Sensing Platform Based on Aptamer-Functionalized DNA Hydrogels for Visual and Fluorescence Detection of Chloramphenicol and Aflatoxin M1

“…There have been also detection methods with similar sensitivity to this study in the literature. Chen et al 61 reported that the fluorescence quenching of quantum dots (QDs) by electron transfer reaction between chloramphenicol and QDs upon specific recognition of chloramphenicol by the molecularly imprinted polymer provided a detection limit of 0.35 ng/mL, while Ali et al 62 introduced the use of the Nhydroxysuccinimide cross-linked graphene oxide−gold nanoflower-modified SPE electrode with a detection limit of 1 nM (0.32 ng/mL) for chloramphenicol detection.…”

Preparation of Molecularly Imprinted Cysteine Modified Zinc Sulfide Quantum Dots Based Sensor for Rapid Detection of Dopamine Hydrochloride

“…The constructed MIP@ZnS QDs integrates the fluorescent characteristic of ZnS QDs and the selective molecular recognition capability of MIPs. The MIP@ZnS QDs has good physical/chemical stability, easy elution/adsorption, fast mass transfer, and high selectivity for target molecules [33][34][35][36][37]. The MIP layer on the QDs can specifically bind and capture target molecules, which will result in fluorescence quenching of quantum dots due to the non-radiative transition between the bound target molecules and quantum dots [38][39][40].…”