Molecularly imprinted upconversion nanoparticles for highly selective and sensitive sensing of Cytochrome c

Guo, Ting; Deng, Qiling; Fang, Guozhen; Liu, Cuicui; Huang, Xuan; Wang, Shuo

doi:10.1016/j.bios.2015.06.058

Cited by 74 publications

(25 citation statements)

References 36 publications

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“…Finally, we chose 40 min as the response time to ensure that MIP-coated UCNPs adsorb as much DES as possible. The adsorption rate of MIP-coated UCNPs was signicantly higher than that previously reported, 32 which was attributed to the surface gra molecularly imprinting method. The higher adsorption rate meant that MIP-coated UCNPs have promising applications in on-eld detection.…”

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confidence: 47%

A label-free detection of diethylstilbestrol based on molecularly imprinted polymer-coated upconversion nanoparticles obtained by surface grafting

et al. 2017

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Herein, we report a novel fluorescent sensor based on molecularly imprinted polymer-coated upconversion nanoparticles (MIP-coated UCNPs) for the recognition and detection of diethylstilbestrol (DES). We used one-step process to modify the surface of the UCNPs with alkenyl groups, which was not only a simple process, but also greatly saved the preparation time. Then, a molecularly imprinted polymer was synthesized on the UCNPs by the surface-graft molecular imprinting method. MIP-coated UCNPs combine the advantages of UCNPs and molecularly imprinted polymers such as strong fluorescence properties, excellent stability, reusability, good adsorption capacity, and high specificity.After the removal of the template molecules, the UCNPs were able to selectively recognize DES, and the fluorescence intensity decreased as the concentration of DES increased. A good linear relationship was obtained in the concentration range of 50-1000 ng mL À1 with the correlation coefficient of 0.9989, and the detection limit was 12.8 ng mL À1 (S/N ¼ 3). MIP-coated UCNPs could be applied for the detection of DES in real milk samples, and the average recoveries for five different concentrations ranged from 90.3%to 107.8%, with the relative standard deviations (RSD) below 2.7%. This fluorescence sensor provides a more convenient, simple and highly specific method for the on-field detection of DES and is promising for a wide range of applications in the future.

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contrasting

confidence: 47%

A label-free detection of diethylstilbestrol based on molecularly imprinted polymer-coated upconversion nanoparticles obtained by surface grafting

et al. 2017

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“…Therefore, this approach is well suited for imprinting of biomacromolecules like proteins . Depending on application modes, the solid supports subjected to surface imprinting of proteins can be nanomaterials , electrodes , microspheres , membranes , and monoliths. Molecular imprinting of proteins in polymers attached to the surface of nanomaterials is currently a hot topic; however, this application mainly focuses on the fabrication of biosensors since the nanomaterials exhibit a remarkably large surface‐to‐volume ratio and size‐related physical and chemical properties.…”

Section: Introductionmentioning

confidence: 99%

“Smart” molecularly imprinted monoliths for the selective capture and easy release of proteins

Wen

Tan

Sun

et al. 2016

J of Separation Science

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A new thermally switchable molecularly imprinted monolith for the selective capture and release of proteins has been designed. First, a generic poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith reacted with ethylenediamine followed by functionalization with 2-bromoisobutyryl bromide to introduce the initiator for atom transfer radical polymerization. Subsequently, a protein-imprinted poly(N-isopropylacrylamide) layer was grafted onto the surface of the monolithic matrix by atom transfer radical polymerization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy of the cross-sections of imprinted monoliths confirmed the formation of dense poly(N-isopropylacrylamide) brushes on the pore surface. The imprinted monolith exhibited high specificity and selectivity toward its template protein myoglobin over competing proteins and a remarkably large maximum adsorption capacity of 1641 mg/g. Moreover, this "smart" imprinted monolith featured thermally responsive characteristics that enabled selective capture and easy release of proteins triggered only by change in temperature with water as the mobile phase and avoided use of stronger organic solvents or change in ionic strength and pH.

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“…Cyt c is known to be released from cells during apoptosis, resulting in it being a key biomarker in monitoring and understanding a number of diseases on the cellular scale . These features have led to vitB12, and cyt c, being sought as targets for a number of biosensor systems. Both vitB12 and cyt c have an absorbance band that overlaps with the 525 and 545 nm emission bands of NP2‐Er , indicating that the presence of both analytes should be detectable by monitoring the change in the ratio between the 525 and 545 nm bands vs. the 660 nm band, which displays negligible spectral overlap in both cases (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Expanding the Scope of Biomolecule Monitoring with Ratiometric Signaling from Rare‐Earth Upconverting Phosphors

et al. 2017

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Upconversion (UC) is a powerful mulitphoton mechanism that converts low-energy photons into higher energy emission. One of the most investigated UC systems is upconverting phosphors (UCPs). Here, a new, one-pot synthetic procedure was used to prepare water dispersible, visibly emissive, rare-earth doped UCPs that were capped with the functional groups oleic acid (OA), 6-aminohexanoic acid (AHA), and 6-maleimidohexanoic acid (MHA). These synthesized UCPs were characterized by UC luminescence, dynamic light scattering (DLS), transmission electron microscopy (TEM), and powder Xray diffraction (pXRD). This study expands upon our previous

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Molecularly imprinted upconversion nanoparticles for highly selective and sensitive sensing of Cytochrome c

Cited by 74 publications

References 36 publications

A label-free detection of diethylstilbestrol based on molecularly imprinted polymer-coated upconversion nanoparticles obtained by surface grafting

A label-free detection of diethylstilbestrol based on molecularly imprinted polymer-coated upconversion nanoparticles obtained by surface grafting

“Smart” molecularly imprinted monoliths for the selective capture and easy release of proteins

Expanding the Scope of Biomolecule Monitoring with Ratiometric Signaling from Rare‐Earth Upconverting Phosphors

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