Molecules on the Surface of Parasitic Nematodes as Probes of the Immune Response in Infection

Maizels, R M; Philipp, Mario T.; Ogilvie, Bridget M.

doi:10.1111/j.1600-065x.1982.tb00375.x

Cited by 89 publications

(43 citation statements)

References 83 publications

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“…In different nematodes proteins of collagenous and noncollagenous origin have been reported to form a part of the surface structure. Some of these constituents are highly cross-linked by disulphide bridges and can be glycosylated (Lumsden, 1975;Adams, 1978;Bird, 1980;Clark, Philipp & Parkhouse, 1982;Maizels et al 1982;Murray, Waite, Tanzer & Hauschka, 1982;Philipp & Rumjaneck, 1984). Moreover, a surface-specific antigen prepared from D. viteae binds strongly to lectins specific for oc iV-acetyl-galactosamine and for fucose (Baschong, unpublished results).…”

Section: Discussionmentioning

confidence: 99%

“…The occurrence of stage-specific surface antigens as observed for larvae of Dipetalonema viteae (Filarioidea) by Weiss & Tanner (1981) could mean that the structure of the contact site changes during development into the adult worm. Stageand environment-dependent alterations of the composition of the cuticle are also indicated for other parasitic nematodes (Philipp, Parkhouse & Ogilvie, 1980;Maizels et al 1982;and very recently, Canlas & Piessens, 1984;Ortega-Pierres, Chayen, Clark & Parkhouse, 1984;Philipp & Rumjaneck, 1984). A consequence of these structural changes, in or on the surface, is the exposure of a different set of potential antigens to the host's immunological system at each stage.…”

Section: Introductionmentioning

confidence: 91%

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Changes in the surface ofDipetalonema viteae(Filarioidea) during its development as shown by comparative peptide mapping

Baschong

1985

Parasitology

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SUMMARYThe cuticle of parasitic nematodes, the main contact site with the host, plays an important role in host-parasite interaction and thus also in immunological control. We compared different surface-iodinated life-stages of the filarial worm Dipetalonema viteae (microfilariae, infective 3rd-stage larvae (L 3 ), adult males and females) with respect to changes in their surface composition. Autoradiographs of peptide maps show that all stages present an identical set of peptide spots reflecting common surface protein(s). Spots specific for larvae L 3 show that the composition of the iodinated surface differs in microfilariae and adults i.e. it changes during development. Adults show a spot typical for males or females. Identical spots are found in L 3 . This suggests that a surface component is also sex specific.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Changes in the surface ofDipetalonema viteae(Filarioidea) during its development as shown by comparative peptide mapping

Baschong

1985

Parasitology

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“…However, molecules derived from the tegument or cuticle of helminths make a substantial contribution to the contents of excretory/secretory products of the parasite maintained in vitro, and are probably also released in vivo (Lightowlers & Rickard 1988). The active release of antigens from the surface of helminths should have a potentially significant impact on the immunobiology of the helminth-host relationship throught their potential triggering effect on the immune system of infected hosts (Kaushal et al 1982, Maizels et al 1982, Butterworth 1984, Lightowlers & Rickard 1988, Matsumoto et al 1988.…”

Section: Discussionmentioning

confidence: 99%

“…Products released by worms as excretory/secretory antigens have been considered as important source of antigens capable of generating immune response in hosts (Maizels et al 1982, Lightowlers & Rickard 1988, Maizels & Selkirk 1988, Lightowlers 1996, Wilson & Coulson 1998, Maizels et al 1999.…”

Section: Discussionmentioning

confidence: 99%

Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract

Thaumaturgo

Vilar

Diogo

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…In contrast to these conserved structural elements, the nematode cuticle exhibits an extremely restricted profile of soluble constituent proteins that may be species-or stagespecific (3,4). The major protein of adult lymphatic filarial parasites defined by surface labeling is a 29-kDa glycoprotein (gp29) that appears to be expressed after infection of the mammalian host (5)(6)(7)(8)(9)(10)(11).…”

mentioning

confidence: 99%

Identification of the major soluble cuticular glycoprotein of lymphatic filarial nematode parasites (gp29) as a secretory homolog of glutathione peroxidase.

Cookson

Blaxter

Selkirk

1992

Proc. Natl. Acad. Sci. U.S.A.

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Lymphatic filariasis is a serious debilitating disease that affects some 90 million people worldwide (1). The causative agents, filarial nematodes of the genera Brugia and Wuchereria, are parasites that persist for long periods of time in the human lymphatic system. They achieve this longevity despite a vigorous host immune response and their apparent inability to undergo antigenic variation, implying a sustained resistance to or subversion of host immunity. All nematodes are bounded by a cuticle, an extracellular matrix composed predominantly of collagens, and a highly cross-linked external envelope of proteins termed cuticlin (2, 3). In contrast to these conserved structural elements, the nematode cuticle exhibits an extremely restricted profile of soluble constituent proteins that may be species-or stagespecific (3, 4). The major protein of adult lymphatic filarial parasites defined by surface labeling is a 29-kDa glycoprotein (gp29) that appears to be expressed after infection of the mammalian host (5-11). Peptide mapping has shown that gp29 from Brugia pahangi and Brugia malayi are highly homologous (8), and the 29-kDa surface-labeled protein from Brugia timori (5) and Wuchereria bancrofti (6) probably represents the same molecule. In adult B. malayi gp29 is synthesized in the syncytial hypodermis, exported to the cuticle, and released into the external environment during in vitro culture (9, 10).We report here the isolation and sequence of cDNAs that encode gp29 from B. pahangi,t which reveals that this cuticular protein is a homolog of the antioxidant enzyme glutathione peroxidase (GSHPx). A possible biological role for this enzyme in defense against immune-mediated cytotoxicity is discussed. MATERIALS AND METHODSParasites. B. malayi were obtained from TRS laboratories, Athens, GA. Adult worms were recovered from the peritoneal cavities ofjirds (Meriones unguiculatus) infected over 3 mo previously with 200 infective larvae and washed extensively with phosphate-buffered saline.Labeling Procedures and Immunochemical Analysis. Adult B. malayi (mixed sex) were extrinsically labeled with BoltonHunter reagent and Iodo-Gen as described elsewhere (12). Parasites were also metabolically labeled with sodium[5S]selenite at 0.25 mCi ml-1 (Amersham SCS-1; 1 Ci = 37 GBq) via in vitro culture for 24 hr in RPMI 1640 medium/2 mM glutamine/1% glucose/penicillin at 100 units-ml-'/ streptomycin at 100 ,ugml-1. All labeled preparations were homogenized in phosphate-buffered saline/1.5% 1-octyl glucoside containing a mixture of protease inhibitors (12) and then centrifuged at 11,000 x g. Immunoprecipitation assays were done with 2.5 ,ul of antiserum in a total volume of 50 ,ul as described (12), resolved on 7-25% gradient polyacrylamide gels, and autoradiographed.cDNA Isolation, Confirmation, and Sequence Determination. A cDNA library in bacteriophage Agtll constructed from mRNA of mixed adult B. pahangi (13) was screened with a polyclonal antiserum to gp29 (9). Clones that reacted positively against gp29 in immunoprecipitatio...

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Molecules on the Surface of Parasitic Nematodes as Probes of the Immune Response in Infection

Cited by 89 publications

References 83 publications

Changes in the surface ofDipetalonema viteae(Filarioidea) during its development as shown by comparative peptide mapping

Changes in the surface ofDipetalonema viteae(Filarioidea) during its development as shown by comparative peptide mapping

Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract

Identification of the major soluble cuticular glycoprotein of lymphatic filarial nematode parasites (gp29) as a secretory homolog of glutathione peroxidase.

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