Molt-cycle correlated patterns of synthesis of integumentary proteins in the land crab Gecarcinus lateralis

Stringfellow, L.; Skinner, Dorothy M.

doi:10.1016/0012-1606(88)90271-0

Cited by 25 publications

(11 citation statements)

References 37 publications

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“…The premolt stage comprises the preparatory events required for ecdysis, such as an increase in circulating ecdysteroids, separation of the old cuticle from the underlying hypodermis (apolysis), and, in some crustacean species, the accumulation of calcium in the gastroliths, paired disk-like calcium-storage organs enveloped by epithelial cells, which are situated in the cardiac stomach wall (McWhinnie, 1962;Travis, 1960;Ueno, 1980). The surge in circulating ecdysteroids triggers a programmed sequence of expression of different genes and synthesis of proteins in a variety of tissues, including the hypodermis and the hepatopancreas (Stringfellow and Skinner, 1988;Traub et al, 1987). This increase in circulating ecdysteroids is followed by a decrease in the levels of circulating ecdysteroids just before ecdysis, a step that is essential for the shedding of the cuticle; with ecdysis being delayed in the absence of theses changes in the ecdysteroids levels (Chang, 1993).…”

Section: Introductionmentioning

confidence: 99%

Search for hepatopancreatic ecdysteroid-responsive genes during the crayfish molt cycle: from a single gene to multigenicity

Shechter

Tom

Yudkovski

et al. 2007

Journal of Experimental Biology

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SUMMARY The expression of the vitellogenin gene of the red-claw crayfish Cherax quadricarinatus (CqVg) was previously demonstrated in male crayfish during an endocrinologically induced molt cycle. The hypothesis that this expression is under the direct control of ecdysteroids was tested in this study both in vivo and in vitro. Unlike vitellogenin of insects, CqVg was not found to be ecdysteroid-responsive. Thus, a multigenic approach was employed for the identification of other hepatopancreatic ecdysteroid-responsive genes by a cDNA microarray. For the purposes of this study, a multi-parametric molt-staging technique, based on X-ray detection of gastrolith growth, was developed. To identify ecdysteroid-responsive genes during premolt, the molt cycle was induced by two manipulations, 20-hydroxyecdysone administration and X-organ–sinus gland complex removal; both resulted in significant elevation of ecdysteroids. Two clusters of affected genes (129 and 122 genes, respectively) were revealed by the microarray. It is suggested that only genes belonging to similarly responsive (up- or downregulated) gene clusters in both manipulations (102 genes) could be considered putative ecdysteroid-responsive genes. Some of these ecdysteroid-responsive genes showed homology to genes controlling chitin metabolism, proteases and other cellular activities, while 56.8% were unknown. The majority of the genes were downregulated, presumably by an energetic shift of the hepatopancreas prior to ecdysis. The effect of 20-hydroxyecdysone on representative genes from this group was confirmed in vitro using a hepatopancreas tissue culture. This approach for ecdysteroid-responsive gene identification could also be implemented in other tissues for the elucidation of ecdysteroid-specific signaling pathways during the crustacean molt cycle.

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Section: Introductionmentioning

confidence: 99%

Search for hepatopancreatic ecdysteroid-responsive genes during the crayfish molt cycle: from a single gene to multigenicity

Shechter

Tom

Yudkovski

et al. 2007

Journal of Experimental Biology

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“…We show electrophoretic patterns of proteins and compare the proteins present in anecdysial, proecdysial, and metecdysial exoskeletons and exuviae of G. lateralis. The distribution of a number of the proteins in one or more layers of the exoskeleton (data shown here), as well as the patterns of their synthesis (Stringfellow and Skinner, 1988;Paulson and Skinner, 1991 ) and degradation Skinner, 1987, 1988), make them favorable candidates for the investigation of genes specifically activated during the molting process.…”

Section: Introductionmentioning

confidence: 79%

“…Gel electrophoresis A discontinuous gel system was used for ID gels (Laemmli, 1970). Separating gels were gradients of 5-15% polyacrylamide (Stringfellow and Skinner, 1988) or 9-18% polyacrylamide, the latter contained 0-10% glycerol (O'Brien and Skinner, 1988); stacking gels were 4.5%. acrylamide.…”

Section: Extraction Of Proteinsmentioning

confidence: 99%

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Proteins of Crustacean Exoskeletons: I. Similarities and Differences among Proteins of the Four Exoskeletal Layers of Four Brachyurans

O'brien

Kumari

Skinner

1991

The Biological Bulletin

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Most of the proteins extracted from exocuticle, endocuticle, and membranous layer of four species of anecdysial (intermolt) crabs (the Bermuda land crab Gecarcinus lateralis, the rock crab Cancer antennarius, the shield-backed kelp crab Pugettia producta, and the southern shield-backed kelp crab Taliepus nuttalli) were 31 kDa or smaller; proteins of similar Mr were common to all three layers. Proteins from the membranous layer were qualitatively indistinguishable in all four species. More proteins 31 kDa or smaller were similar in size and pI to proteins from other exoskeletal layers than were proteins larger than 31 kDa. Proteins extracted from the epicuticle of G. lateralis included a group of five ranging from 54 to 42 kDa that bound 45Ca++ in vitro. The group was not seen in other layers of the exoskeleton of G. lateralis or, with the exception of 44 and 42 kDa protein bands that were in the epicuticle of C. antennarius, in any layers of the exoskeletons of the other three species. During proecdysis, the membranous layer is completely degraded, and proteins 31 kDa or smaller are preferentially degraded from the exocuticle and endocuticle of the old exoskeleton of G. lateralis, which is cast as an exuvia at ecdysis. The relative amounts of proteins in extracts of epicuticle from (1) anecdysial exoskeletons and (2) exuviae were very similar, suggesting that there was little degradation of epicuticle during proecdysis. Some of the proteins of the three inner layers of the exoskeleton of G. lateralis have characteristics similar to those of flexible cuticles of insects; they have acidic pIs and they form "charge trains," i.e., proteins of the same size separated by differences in charge during isoelectric focusing.

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“…Ecdysteroids, the moulting hormones common to all arthropods, are synthesized in the crustacean Y-organs, endocrine steroidogenic glands of ectodermal origin (reviewed in Lachaise et al, 1993). The increase in ecdysteroid synthesis at the onset of premoult leads to a surge of circulating 20-hydroxyecdysone (20E) that triggers a programmed synthesis of proteins in the epidermis and the hepatopancreas (Bielefeld et al, 1986;Traub et al, 1987;Stringfellow & Skinner, 1988). For successful ecdysis, a decrease in the 20E level is required immediately prior to moulting; without this drop ecdysis is delayed (Chang et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Hepatopancreatic multi‐transcript expression patterns in the crayfish Cherax quadricarinatus during the moult cycle

Yudkovski

Shechter

Chalifa‐Caspi

et al. 2007

Insect Molecular Biology

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Alterations of hepatopancreatic multi-transcript expression patterns, related to induced moult cycle, were identified in male Cherax quadricarinatus through cDNA microarray hybridizations of hepatopancreatic transcript populations. Moult was induced by X-organ sinus gland extirpation or by repeated injections of 20-hydroxyecdysone. Manipulated males were sacrificed at premoult or early postmoult, and a reference population was sacrificed at intermoult. Differentially expressed genes among the four combinations of two induction methods and two moult stages were identified. Biologically interesting clusters revealing concurrently changing transcript expressions across treatments were selected, characterized by a general shift of expression throughout premoult and early postmoult vs. intermoult, or by different premoult vs. postmoult expressions. A number of genes were differentially expressed in 20-hydroxyecdysone-injected crayfish vs. X-organ sinus gland extirpated males.

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Molt-cycle correlated patterns of synthesis of integumentary proteins in the land crab Gecarcinus lateralis

Cited by 25 publications

References 37 publications

Search for hepatopancreatic ecdysteroid-responsive genes during the crayfish molt cycle: from a single gene to multigenicity

Search for hepatopancreatic ecdysteroid-responsive genes during the crayfish molt cycle: from a single gene to multigenicity

Proteins of Crustacean Exoskeletons: I. Similarities and Differences among Proteins of the Four Exoskeletal Layers of Four Brachyurans

Hepatopancreatic multi‐transcript expression patterns in the crayfish Cherax quadricarinatus during the moult cycle

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