Twenty naturally occurring strains of Bradyrhizobium japonicum in 11 serogroups were screened for the ability to take up Mo as bacteroids from soybean root nodules. The strains varied greatly in their ability to take up Mo in a 1-min period. The best strain was USDA 136, which had an Mo uptake activity of almost 3.0 pmol/min per mg of bacteroid (dry weight). In contrast, the poorest strain, USDA 62, had an Mo uptake activity of 0. The dependence on molybdenum for nitrogen fixation was first demonstrated by,Bortels (5), who showed that Mo was required for growth of Azotobacter vinelandii on dinitrogen but not for growth on ammonium. Many years later the biochemical basis for this dependency on Mo was demonstrated by Bulen and LeComte (6), who showed that purified nitrogenase contained Mo. This is apparently true for the nitrogenase from all sources of the varied bacteria able to fix N2. However, there is evidence that A. vinelandii contains another nitrogenase whose activity is independent of Mo (4).The two atoms of Mo contained by component I of nitrogenase are associated with the active site of the enzyme (33).It has been demonstrated that molybdenum is a required trace element for symbiotic N2 fixation as well. Anderson (1,2) found that the addition of Mo to N2-fixing clover resulted in significant increases in plant dry weight. Since then there have been many reports of increased legume dry weight (8-10, 17, 24, 26, 29, 30) and increased symbiotic nitrogen fixation activity (8,9,20,24)