Monitoring biomass in root culture systems

Ramakrishnan, Divakar; Luyk, Derek; Curtis, Wayne R.

doi:10.1002/(sici)1097-0290(19990320)62:6<711::aid-bit10>3.0.co;2-0

Cited by 28 publications

(12 citation statements)

References 27 publications

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“…In this case also Y = mX + C form was employed for a better correlation as reported by Ballica et al [21]. Osmolairty has been used as a method for monitoring biomass in hairy root cultures of Hyoscyamus muticus grown in bioreactors and found that the results were well corroborated with that of the experimental [23]. In the present study, the biomass values of different bioreactors employed are predicted using an empirical equation obtained based on the shake flask data [18].…”

Section: Conductivity and Osmolarity Measurements In Bioreactorsmentioning

confidence: 59%

Comparative evaluation of bioreactor design using Tagetes patula L. hairy roots as a model system

Suresh

Bais

Raghavarao

et al. 2005

Process Biochemistry

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Section: Conductivity and Osmolarity Measurements In Bioreactorsmentioning

confidence: 59%

Comparative evaluation of bioreactor design using Tagetes patula L. hairy roots as a model system

Suresh

Bais

Raghavarao

et al. 2005

Process Biochemistry

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“…Additional practical considerations are (1) the host cell morphology and aggregate size distribution, since large aggregates can clog the cell compartment port during inoculation (and/or suspension removal), (2) membrane strength to endure cell compartment expansion resulting from osmotic flux and cell aggregate expansion during long-term operation, and (3) difficulty in monitoring biomass concentration and/or viability in packed bed operation. To address the latter challenges, it may be possible to adapt techniques for estimation of viable biomass in root culture systems that utilize changes in the medium refractive index, conductivity, and/or osmolality (42) or correlations based on sugar and/or oxygen uptake rates. For assessment of culture viability, it may be possible to develop correlations between esterase activity in the cell compartment medium and rice culture viability using staining methods as has been previously described by Steward and co-workers for alfalfa cell suspension cultures (43).…”

Section: Discussionmentioning

confidence: 99%

Production of Human α-1-Antitrypsin from Transgenic Rice Cell Culture in a Membrane Bioreactor

McDonald

Hong

Trombly

et al. 2008

Biotechnol Progress

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Transgenic plant cell cultures offer a number of advantages over alternative host expression systems, but so far relatively low product concentrations have been achieved. In this study, transgenic rice cells are used in a two-compartment membrane bioreactor (CELLine 350, Integra Biosciences) for the production of recombinant alpha-1-antitrypsin (rAAT). Expression of rAAT is controlled by the rice alpha-amylase (RAmy3D) promoter, which is induced in the absence of sugar. The extracellular product is retained in the bioreactor's relatively small cell compartment, thereby increasing product concentration. Due to the packed nature of the cell aggregates in the cell compartment, a clarified product solution can be withdrawn from the bioreactor. Active rAAT reached levels of 100-247 mg/L (4-10% of the total extracellular protein) in the cell compartment at 5-6 days postinduction, and multiple inductions of the RAmy3D promoter were demonstrated.

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“…The model incorporates the general features of hairy root growth that have been reported in the literature (6,(20)(21)(22)(23). In particular, the effects of cell division leading to lengthening of an existing branch as well as splitting leading to the formation of new branches are included.…”

Section: Discussionmentioning

confidence: 99%

“…Figures 1 and 2 give the experimentally determined biomass as a function of culturing time for six hairy roots: Helianthus annus, Hyoscyamus muticus, Catharanthus roseus, Trigonella foenum-graecum, Daucus carota, and Ajuga sp. (6,(20)(21)(22)(23). Among them, results for H. annus were obtained in our laboratory, and the other results were taken from the literature.…”

Section: Introductionmentioning

confidence: 99%

Population Balance Approach to Modeling Hairy Root Growth

et al. 2004

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Though numerous models have been developed to describe the growth of microbial cell cultures, far fewer models are available to describe the growth of hairy root cultures. Here a population balance model is proposed to simulate the growth of hairy roots. The model accounts for the increase in biomass due to elongation of a branch by cell division as well as the formation of new branches. The model incorporates the fact that although the likelihood of the formation of a new lateral branch is a maximum at a specific age of the parent branch, lateral branches can form over a distribution of ages of the parent branch. Model parameters are estimated using the genetic algorithm based on experimental data for batch and continuous bioreactors. The model proposed here may provide a better understanding of the increase in biomass of hairy root cultures.

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Monitoring biomass in root culture systems

Cited by 28 publications

References 27 publications

Comparative evaluation of bioreactor design using Tagetes patula L. hairy roots as a model system

Comparative evaluation of bioreactor design using Tagetes patula L. hairy roots as a model system

Production of Human α-1-Antitrypsin from Transgenic Rice Cell Culture in a Membrane Bioreactor

Population Balance Approach to Modeling Hairy Root Growth

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