Population Balance Approach to Modeling Hairy Root Growth

Han, Binbing; Linden, James C.; Gujarathi, Ninad P.; Wickramasinghe, S. Ranil

doi:10.1021/bp0342304

Cited by 18 publications

(11 citation statements)

References 22 publications

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“…Cell size did not correlate in our study with the rate of root elongation. This may be due to the in vitro effect on the propagation of root segments, rather than whole plants (Han et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato

Gal

Aussenberg

Burdman

et al. 2006

Planta

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A group of plant proteins, expansins, have been identified as wall-loosening factors and as facilitators of cell expansion in vivo. The root knot nematode Meloidogyne javanica establishes a permanent feeding site composed of giant cells surrounded by gall tissue. We used quantitative PCR and in situ localization to demonstrate the induction of a tomato (Lycopersicon esculentum cv. VF36) expansin (LeEXPA5) expression in gall cells adjacent to the nematode feeding cells. To further characterize the biological role of LeEXPA5 we have generated LeEXPA5-antisense transgenic roots. The ability of the nematode to establish a feeding site and complete its life cycle, the average root cell size and the rate of root elongation were determined for the transgenic roots, as well as the level of LeEXPA5 expression in non-infected and nematode-infected roots. Our results demonstrated that a decrease of LeEXPA5 expression reduces the ability of the nematode to complete its life cycle in transgenic roots. We suggest that a plant-originated expansin is necessary for a successful parasitic nematode-plant interaction.

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Section: Discussionmentioning

confidence: 99%

Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato

Gal

Aussenberg

Burdman

et al. 2006

Planta

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“…Our previous studies have revealed that oxidative burst, NO (nitric oxide) generation, RGD (Arg-Gly-Asp)-binding proteins, mitogen-activated protein kinase-like cascades and phospholipid signalling play important roles in the response of Taxus (yew) cells to shear stress [6][7][8][9][10][11][12]. These events were all closely related to the properties of cell membranes.…”

Section: Introductionmentioning

confidence: 99%

Analysis of phospholipids, sterols, and fatty acids in Taxus chinensis var. mairei cells in response to shear stress

Han

Zhou

Yang

2009

Biotech and App Biochem

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Plant cell culture has been developed as an alternative method for the production of an anticancer drug, paclitaxel. However, the sensitivity of plant cells to shear stress has been one of the main obstacles to the scale-up of the plant cell culture. To gain a better understanding of the mechanism of plant cells' response to hydrodynamic mechanical stress, lipid profiling of suspension-cultured Taxus chinensis (Chinese yew) var. mairei cells under shear stress was carried out using liquid chromatography-tandem MS and gas chromatography-time-of-flight MS. T. chinensis var. mairei cells cultured in a Couette-type shear reactor responded with an increase of cell membrane permeability compared with control cells, which indicated that the adaptation to shear stress altered membrane lipid composition. The main changes of lipid profiles in the shear-stress-induced cells were the following: (a) the total phospholipid content decreased, especially that of structural phospholipids such as phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; (b) cells showed increased stigmasterol at the expense of sitosterol and campesterol; (c) the stigmasterol/phospholipid ratio increased; (d) the mono-unsaturated-fatty-acid content increased; (e) the shear-stress-induced cells accumulated very-long-chain saturated fatty acids (C22:0, C23:0, C24:0 and C25:0). These findings suggested alterations in membrane composition and hinted at a possible molecular basis for the mechanotransduction processes induced by shear stress in T. chinensis var. mairei cells.

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“…Modeling the tissue growth by measuring osmolality (concentrations of different components) and the conductivity of the medium during culture are the examples of mathematical estimation of root growth through fresh weight time course data (Leduc et al 2006;Cloutier et al 2008). Another relevant example that uses the mathematical calculation to study the biomass increment based on population balance approach has also been proposed (Han et al 2004). Such examples of inclusion of interdisciplinary approach would definitely provide new dynamism and dimensions to the HRBP.…”

Section: Growth Simulations and Modelingmentioning

confidence: 99%

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

et al. 2015

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Agrobacterium rhizogenes-mediated hairy roots (HR) were developed in the laboratory to mimic the natural phenomenon of bacterial gene transfer and occurrence of disease syndrome. The timeline analysis revealed that during 90 s, the research expanded to the hairy root-based secondary metabolite production and different yield enhancement strategies like media optimization, up-scaling, metabolic engineering etc. An outlook indicates that much emphasis has been given to the strategies that are helpful in making this technology more practical in terms of high productivity at low cost. However, a sequential analysis of literature shows that this technique is upgraded to a biotechnology platform where different intra- and interdisciplinary work areas were established, progressed, and diverged to provide scientific benefits of various hairy root-based applications like phytoremediation, molecular farming, biotransformation, etc. In the present scenario, this biotechnology research platform includes (a) elemental research like hairy root-mediated secondary metabolite production coupled with productivity enhancement strategies and (b) HR-based functional research. The latter comprised of hairy root-based applied aspects such as generation of agro-economical traits in plants, production of high value as well as less hazardous molecules through biotransformation/farming and remediation, respectively. This review presents an indicative timeline portrayal of hairy root research reflected by a chronology of research outputs. The timeline also reveals a progressive trend in the state-of-art global advances in hairy root biotechnology. Furthermore, the review also discusses ideas to explore missing links and to deal with the challenges in future progression and prospects of research in all related fields of this important area of plant biotechnology.

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Population Balance Approach to Modeling Hairy Root Growth

Cited by 18 publications

References 22 publications

Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato

Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato

Analysis of phospholipids, sterols, and fatty acids in Taxus chinensis var. mairei cells in response to shear stress

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

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