Monitoring cell adhesion on tantalum and oxidised polystyrene using a quartz crystal microbalance with dissipation

Lord, Megan S.; Modin, Charlotte; Foss, Morten; Duch, Mogens; Simmons, Anne; Pedersen, Finn Skou; Milthorpe, Bruce; Besenbacher, Flemming

doi:10.1016/j.biomaterials.2006.04.006

Cited by 106 publications

(105 citation statements)

References 41 publications

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“…It is commonly known that several physical and biological cues including ECM protein coating, serum protein, cell type, etc., directly affects the adhesion strength of anchorage-dependent cells on biomaterial surfaces during initial cell seeding [30]. In addition to the pre-culture time of cell, it is likely that those physical and biological factors as mentioned above will influence the cell deadhesion dynamics and resulting cellular phenotypes of cells on HBC29 surface.…”

Section: Resultsmentioning

confidence: 99%

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

Chen¹,

Dang²,

Tan³

et al. 2007

Biomaterials

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Thermoresponsive polymer (TRP) enables the enzyme-free harvesting of cells through an acute increase in surface hydrophilicity of TRP across its lower critical solution temperature (LCST), rendering feasible the generation of polymer-free cell sheets for regenerative medicine applications. To date, the intricate mechanisms of cell deadhesion/detachment on TRP surface remain obscure. Elucidation of such biophysical responses would be valuable for the cell sheet technology. In this study, integrative biophysical techniques are applied to probe the thermal-induced deadhesion kinetics of smooth muscle cell (SMC) on thermoresponsive hydroxybutyl chitosan (HBC29) against different periods of pre-culture time at 37 °C. Atomic force microscopy demonstrates that both the surface topography and mechanical property of HBC29 film in water are acutely modulated across its LCST. Firstly, cells show negligible changes in adhesion contact area during low-temperature incubation on unmodified tissue culture polystyrene (TCPS). Secondly, the recession of adhesion contact and retraction of cell body for cells with different pre-culture times are triggered by HBC29 coating on TCPS. Interestingly, the initial rate of reduction in the normalized adhesion contact area of SMC is negatively correlated with the pre-culture time. Thirdly, the degree of cell deformation and average adhesion energy are reducing functions of time only for SMCs with the lowest preculture time. In contrast, adhesion energy per cell is a reducing function of time irrespective of the change of pre-culture time. Lastly, the temporal dynamics of cytoskeleton organization and β-actin/ smoothelin-B mRNA expression for SMCs is strongly dependent on the pre-culture time. Overall, this study demonstrates that the thermal-induced deadhesion of SMC on TRP is characterized by the evolution of its contractile phenotypes.

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Section: Resultsmentioning

confidence: 99%

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

Chen¹,

Dang²,

Tan³

et al. 2007

Biomaterials

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“…As shown in many works, the QCM sensor has been proved to be a good immunosensor [15][16][17][18][19][20]. Commercial quartz crystal resonator with polystyrene coating has been used successfully and developed as a basis for QCM Immunosensor [21][22][23][24][25]. In this work, we present a candidate tool to identify the existence of cow milk in solution by utilizing a quartz crystal microbalance coated with polystyrene.…”

Section: Introductionmentioning

confidence: 99%

Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

et al. 2016

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Adulteration of goat milk is usually done using cow's milk product. Cow milk is used as it is widely available and its price is cheaper compared to goat milk. This paper shows a development of candidate tools for milk adulteration using cow milk. A quartz crystal microbalance immunosensor was developed using commercial crystal resonator and polyclonal antibody specific to cow milk protein. A specific protein at 208 KDa is found only in cow milk and does not exist in goat milk. The existence of this protein can be used as an indicator of cow milk content in a target solution. To detect the PSS 208 kDa protein, antibody specific to the PSS 208 was developed. The purified antibody was immobilized on top of the sensor surface on a polystyrene layer. The fraction of the immobilized antibody on the sensor was found at 1.5% of the given antibody. Using a static reaction cell, the developed immunosensor could detect the specific cow milk protein in buffer solution. The detection limit is 1 ppm. A linear relationship between frequency change and specific protein of cow milk concentration is found from a concentration of 1 ppm to 120 ppm.

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“…The formation of pseudopodia would fall within the detection range and could therefore be the main cause of the increase in mass of the QCM-D chip leading to the frequency and dissipation shifts with time. Modin et al [13,24,25] also showed that QCM-D and consequently Df plots can be used to determine the spreading behaviour of cells through the increase in dissipation versus the decrease in frequency upon their contact with different surfaces even with the signal penetration depth limitation. Varying the experimental conditions such as cell type, buffer, temperature, etc., authors could obtain a typical Df plot for each of the given conditions [27] with a general trend of increasing slopes for D and decreasing slopes for f. This feature was then attributed to cell spreading, as cell spreading leads to a progressive increase of viscoelastic mass (owing to high hydration state of cells) on the sensor surface.…”

Section: Monitoring Platelet Activation Rate Using a Supporting Protementioning

confidence: 99%

“…QCM-D has proven to be a simple and effective way to quantitatively study cell adhesion and spreading in situ [13,24,25]. In a previous study [15], we also examined platelet adhesion in a quasithree-dimensional space via subtle cytoskeleton changes, with protein composition of the QCM-D chip biointerface.…”

Section: Introductionmentioning

confidence: 99%

“…Reports are available where reproducible dissipation factor (D) versus frequency ( f ) plots were obtained as a result of cell interactions with surfaces having various physico-chemical properties [13,[24][25][26][27][28]. Thrombogenic human fibronectin (HFN) and non-thrombogenic human serum albumin (HSA) have been employed as model protein biointerface to understand the biochemical processes involved in the QCM-D response during platelet activation.…”

Section: Introductionmentioning

confidence: 99%

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Determination of surface-induced platelet activation by applying time-dependency dissipation factor versus frequency using quartz crystal microbalance with dissipation

Fatisson

Mansouri

Yacoub

et al. 2011

J. R. Soc. Interface.

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Platelet adhesion and activation rates are frequently used to assess the thrombogenicity of biomaterials, which is a crucial step for the development of blood-contacting devices. Until now, electron and confocal microscopes have been used to investigate platelet activation but they failed to characterize this activation quantitatively and in real time. In order to overcome these limitations, quartz crystal microbalance with dissipation (QCM-D) was employed and an explicit time scale introduced in the dissipation versus frequency plots (Df -t) provided us with quantitative data at different stages of platelet activation. The QCM-D chips were coated with thrombogenic and non-thrombogenic model proteins to develop the methodology, further extended to investigate polymer thrombogenicity. Electron microscopy and immunofluorescence labelling were used to validate the QCM-D data and confirmed the relevance of Df-t plots to discriminate the activation rate among protein-modified surfaces. The responses showed the predominant role of surface hydrophobicity and roughness towards platelet activation and thereby towards polymer thrombogenicity. Modelling experimental data obtained with QCM-D with a Matlab code allowed us to define the rate at which mass change occurs (A/B), to obtain an A/B value for each polymer and correlate this value with polymer thrombogenicity.

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Monitoring cell adhesion on tantalum and oxidised polystyrene using a quartz crystal microbalance with dissipation

Cited by 106 publications

References 41 publications

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan

Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

Determination of surface-induced platelet activation by applying time-dependency dissipation factor versus frequency using quartz crystal microbalance with dissipation

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