Monitoring changes in the chemical composition of dissolvable tobacco products

Abstract

Dissolvable tobacco products are smokeless, spit-free, made from finely milled tobacco, and dissolve in the mouth of a user. In 2009, R.J. Reynolds Tobacco Co. released a line of dissolvable tobacco products in several test markets. In late 2010, the dissolvables were reformulated and several changes in their composition were determined and are reported here. Our analytical approach featured gas chromatography-mass spectrometry preceded by four separate methods for sample preparation: solvent extraction using acetone, trimethylsilyl derivatization of sugars, solid-phase microextraction of semivolatiles and liquid-liquid extraction for the quantitative analysis of nicotine. Taken together, these methods identified several changes in the formulation of the initial products and their reformulated versions. One major change in the new dissolvables is that "mellow" and "fresh" flavors have been replaced by a "mint" flavor. Additionally, the strips are no longer dominated by glycerol, but rather threitol. Total nicotine and (free nicotine) concentrations for the orbs, sticks, and strips were found to be 3.35 mg g À1 (1.242 mg g À1 ), 2.6 mg g À1 (0.850 mg g À1 ), and 2.74 mg g À1 (0.572 mg g À1 ), respectively.Total nicotine and free nicotine concentrations have significantly increased in some cases.

Design and Optimization of a Total Vaporization Technique Coupled to Solid-Phase Microextraction

“…The standard solution contained 5 μg/mL nicotine, 5 μg/mL cotinine, and 2 μg/mL quinoline (internal standard) in chloroform. Quinoline has been used as an internal standard for determining nicotine in a number of different tobacco products. − Samples were incubated at 115 °C for 5 min. A polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber was then exposed inside the SPME vial for 60 min.…”

Development of a Cigarette Tobacco Filler Standard Reference Material

“…A variety of approaches are used to characterize the composition of tobacco and tobacco products. Nicotine and related alkaloids are commonly determined by gas chromatography with flame ionization detection (GC-FID), − gas chromatography with mass spectrometric detection (GC-MS), − and liquid chromatography. − Related liquid chromatography mass spectrometric methods have also been reported for the determination of nicotine alkaloids in biological fluids. − Methods for the determination of tobacco-specific nitrosamines (TSNAs) have been reported based on liquid chromatography tandem mass spectrometry (LC-MS/MS). − …”