Monitoring extracellular K+ flux with a valinomycin-coated silicon nanowire field-effect transistor

Chang, Ko-Shing; Sun, Chih-Jung; Chiang, Po-Hsing; Chou, Ai-Chuan; Lin, Ming‐Chung; Chieh, Liang; Hung, Hui-Hsing; Yeh, Yu-Hsiu; Chen, Chii-Dong; Pan, Chien‐Yuan; Chen, Yit‐Tsong

doi:10.1016/j.bios.2011.10.005

Cited by 37 publications

(11 citation statements)

References 56 publications

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“…In addition, ISFET sensors can be fabricated in large numbers using standard lithographic processes. Recent studies have demonstrated the use of ISFETs for measuring ion flux from single cells plated and grown on coated (Straub et al, 2001; Wrobel et al, 2005; Chang et al, 2012) or uncoated (Yu et al, 2007; Gebinoga et al, 2010; Sakata et al, 2012) sensors. However, the application of ISFETs in multi-well plate, parallel measurement configurations for ion efflux analysis has not been determined.…”

Section: Introductionmentioning

confidence: 99%

Application of ion-sensitive field effect transistors for ion channel screening

Walsh

DeRoller

Zhu

et al. 2014

Biosensors and Bioelectronics

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Cell-based screening assays are now widely used for identifying compounds that serve as ion channel modulators. However, instrumentation for the automated, real-time analysis of ion flux from clonal and primary cells is lacking. This study describes the initial development of an ion-sensitive field effect transistor (ISFET)-based screening assay for the acquisition of K+ efflux data from cells cultured in multi-well plates. Silicon-based K+-sensitive ISFETs were tested for their electrical response to varying concentrations of KCl and found to display a linear response relationship to KCl in the range of 10 µM to 1 mM. The ISFETs, along with reference electrodes, were inserted into fast-flow chambers containing either human colonic T84 epithelial cells or U251-MG glioma cells. Application of the Ca2+ ionophore A23187 (1 µM), to activate Ca2+-activated non-selective cation (NSC) channels (T84 cells) and large conductance Ca2+-activated K+ (BK) channels (U251 cells), resulted in time-dependent increases in the extracellular K+ concentration ([K+]o) as measured with the ISFETs. Treatment of the cells with blockers of either the NSC or BK channels, caused a strong inhibition of the A23187-induced increase in [K+]o. These results were consistent with ion current measurements obtained using the whole-cell arrangement of the patch clamp procedure. In addition, K+ efflux data could be acquired in parallel from multiple cell chambers using the ISFET sensors. Given the non-invasive properties of the probes, the ISFET-based assay should be adaptable for screening ion channels in various cell types.

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Section: Introductionmentioning

confidence: 99%

Application of ion-sensitive field effect transistors for ion channel screening

Walsh

DeRoller

Zhu

et al. 2014

Biosensors and Bioelectronics

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“…The abnormal permeability of K + ions was suggested to be related to neural degeneration, diabetes mellitus, and epilepsy (MacDonald et al 2001;Jentsch 2000;Singh et al 1998). K a was determined to be 5,974 ± 115 M −1 , which is significantly higher than the apparent association constants for VAL binding to Li + , Na + , and Cs + ions (Chang et al 2012). To detect K + ions, a polyvinyl chloride (PVC) membrane, embedded with valinomycin (VAL), coated on a SiNW-FET (referred to as VAL-PVC/SiNW-FET) was used to monitor the efflux of K + ions from chromaffin cells (Chang et al 2012).…”

Section: Protein-protein and Protein-small Molecule Interactionsmentioning

confidence: 77%

Nanobiosensors and Nanobioanalyses

Vestergaard¹,

Kerman²,

Hsing³

et al. 2015

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“…The SiNW-FETs were fabricated from silicon-on-insulator (SOI) wafers 17 27 28 37 , composed of a layer of high-quality single-crystal silicon (device layer), a layer of buried oxide (BOX layer), and the bulk substrate. These SOI wafers are currently state-of-the-art in the production of metal-oxide-semiconductor field-effect-transistors (MOSFETs).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Post-Transcriptional Gene Silencing-Related Micro-RNAs by Functionalized Silicon Nanowire Field-effect Transistor

Chen

Pan

et al. 2015

Sci Rep

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Many transcribed RNAs are non-coding RNAs, including microRNAs (miRNAs), which bind to complementary sequences on messenger RNAs to regulate the translation efficacy. Therefore, identifying the miRNAs expressed in cells/organisms aids in understanding genetic control in cells/organisms. In this report, we determined the binding of oligonucleotides to a receptor-modified silicon nanowire field-effect transistor (SiNW-FET) by monitoring the changes in conductance of the SiNW-FET. We first modified a SiNW-FET with a DNA probe to directly and selectively detect the complementary miRNA in cell lysates. This SiNW-FET device has 7-fold higher sensitivity than reverse transcription-quantitative polymerase chain reaction in detecting the corresponding miRNA. Next, we anchored viral p19 proteins, which bind the double-strand small RNAs (ds-sRNAs), on the SiNW-FET. By perfusing the device with synthesized ds-sRNAs of different pairing statuses, the dissociation constants revealed that the nucleotides at the 3′-overhangs and pairings at the terminus are important for the interactions. After perfusing the total RNA mixture extracted from Nicotiana benthamiana across the device, this device could enrich the ds-sRNAs for sequence analysis. Finally, this bionanoelectronic SiNW-FET, which is able to isolate and identify the interacting protein-RNA, adds an additional tool in genomic technology for the future study of direct biomolecular interactions.

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Monitoring extracellular K+ flux with a valinomycin-coated silicon nanowire field-effect transistor

Cited by 37 publications

References 56 publications

Application of ion-sensitive field effect transistors for ion channel screening

Application of ion-sensitive field effect transistors for ion channel screening

Nanobiosensors and Nanobioanalyses

Isolation and Identification of Post-Transcriptional Gene Silencing-Related Micro-RNAs by Functionalized Silicon Nanowire Field-effect Transistor

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