Monitoring for resistant populations of Xanthomonas citri subsp. citri and epiphytic bacteria on citrus trees treated with copper or streptomycin using a new semi-selective medium

Behlau, Franklin; Jones, Jeffrey B.; Myers, Monty E.; Graham, James H.

doi:10.1007/s10658-011-9870-7

Cited by 37 publications

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“…El cobre es uno de los bactericidas más utilizados en todo el mundo, pero diversos experimentos han demostrado la existencia de cepas resistentes a concentraciones bajas de este producto (Quezado et al, 2003;Martin et al, 2004;Maneguim et al, 2007;Behlau et al, 2012). Behlau et al (2012) afirman que, dependiendo de la cepa, hay presencia de genes cop de resistencia a cobre: copL, copA, copB, copM, copG, copC, copD, y copF, y pueden estar localizados en plásmidos o en el cromosoma.…”

Section: Introductionunclassified

“…Behlau et al (2012) afirman que, dependiendo de la cepa, hay presencia de genes cop de resistencia a cobre: copL, copA, copB, copM, copG, copC, copD, y copF, y pueden estar localizados en plásmidos o en el cromosoma.…”

Section: Introductionunclassified

“…y Pseudomonas sp. (Llop et al, 2012;Behlau et al, 2012). Otros casos de resistencia a productos como kasugamicina y validamicina Additional key words: Passiflora edulis Sims, antimicrobial, resistance.…”

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“…La resistencia por parte de los aislamientos a dos de los aminoglucósidos evaluados, sulfato de kanamicina y kasugamicina, se puede relacionar con elementos y mecanismos de resistencia cromosómicos y extracromosómicos (Behlau et al, 2012); los primeros se deben a mutaciones en los loci, que conllevan a cambios en las proteínas ribosómicas en las que se fijan estos antibióti-cos. En el segundo mecanismo se dan elementos móviles de resistencia como los transposones y plásmidos, en donde se encuentran enzimas modificantes (como acetiltrasferasas, nucleotidiltransferasas y fosfotransferasas) que impiden la entrada del aminoglucósido a través de la membrana celular (Dámaso, 1990). Por otra parte, se ha reportado efectividad del control in vitro de kasugamicina sobre aislamientos bacterianos fitopatógenos a concentraciones superiores a las empleadas en este trabajo (500 y 700 mg L -1 ) (Sales et al, 2005).…”

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“…y E. amylovora (Misavage et al, 1990;Spotts y Cervantes, 1995;Russo et al, 2008). Por otra parte, en este trabajo fueron analizadas cepas bacterianas patogénicas y no patogénicas, similar a lo realizado por Behlau et al (2012), quienes realizando aplicaciones de estreptomicina en plantaciones de cítricos afectadas por X. citri encontraron un significativo aumento de bacterias epíficas resistentes o resistentes a este antibiótico en el transcurso del tiempo, debido a la presión de selección para la resistencia bacteriana. Esto, a la larga, representa un riesgo en agricultura por fenómenos de transmisión horizontal de elementos génicos que puedan conferir resistencia.…”

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Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos

Farfán

Benítez

Hoyos-Carvajal

2014

Rev. Colomb. Cienc. Hortic.

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Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos

Farfán

Benítez

Hoyos-Carvajal

2014

Rev. Colomb. Cienc. Hortic.

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Hexyl gallate for the control of citrus canker caused by Xanthomonas citri subsp citri

et al. 2020

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Brazil is the biggest producer of sweet oranges and the main exporter of concentrated orange juice in the world. Among the diseases that affect citriculture, Asiatic citrus canker, caused by the bacterial pathogen Xanthomonas citri, represents one of the most significant threats. The current Brazilian legislation regulating the control of citrus canker no longer requires the eradication of affected trees in states where the incidence of the disease is high. Instead, control involves disease control measures, including periodic preventative spraying of copper compounds. The long‐term use of copper for plant disease control has raised concerns about environmental accumulation and toxicity, as well as the selective pressure it exerts leading to the emergence of copper‐resistant X. citri strains. Here, we evaluated hexyl gallate (G6) as an alternative to copper compounds for citrus plant protection. G6 was able to protect citrus nursery trees against X. citri infection. Thirty days after inoculation, the trees treated with G6 developed 0.5 lesions/cm2 leaf area compared with the 2.84 lesions/cm2 observed in the untreated control trees. Also, G6 did not interfere with germination and root development of tomato, lettuce, and arugula, which is consistent with our previous data showing that G6 is safe for tissue culture cell lines. Membrane permeability tests showed that the primary target of G6 is the bacterial outer membrane. Finally, we could not isolate spontaneous X. citri mutants resistant to G6 nor induce resistance to G6 after long‐term exposures to increasing concentrations of the compound, which suggests that G6 may have multiple cellular targets. This study demonstrated that G6 is a promising candidate for the development and use in citrus canker management.

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Bifunctional peptides as alternatives to copper-based formulations to control citrus canker

Dilarri,

de Lencastre Novaes,

Jakob

et al. 2024

Appl Microbiol Biotechnol

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Citrus canker is an infectious bacterial disease and one of the major threats to the orange juice industry, a multibillion-dollar market that generates hundreds of thousands of jobs worldwide. This disease is caused by the Gram-negative bacterium Xanthomonas citri subsp. citri. In Brazil, the largest producer and exporter of concentrate orange juice, the control of citrus canker is exerted by integrated management practices, in which cupric solutions are intensively used in the orchards to refrain bacterial spreading. Copper ions accumulate and are as heavy metals toxic to the environment. Therefore, the aim of the present work was to evaluate bifunctional fusion proteins (BiFuProts) as novel and bio-/peptide-based alternatives to copper formulations to control citrus canker. BiFuProts are composed of an anchor peptide able to bind to citrus leaves, and an antimicrobial “killer” peptide to protect against bacterial infections of plants. The selected BiFuProt (Mel-CgDEF) was bactericidal against X. citri at 125 μg mL−1, targeting the bacterial cytoplasmic membrane within the first minutes of contact. The results in the greenhouse assays proved that Mel-CgDEF at 250 μg mL−1 provided protection against X. citri infection on the leaves, significantly reducing the number of lesions by area when compared with the controls. Overall, the present work showed that the BiFuProt Mel-CgDEF is a biobased and biodegradable possible alternative for substitute cupric formulations. Key points • The bifunctional fusion protein Mel-CgDEF was effective against Xanthomonas citri. • Mel-CgDEF action mechanism was the disruption of the cytoplasmic membrane. • Mel-CgDEF protected citrus leaves against citrus canker disease.

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Monitoring for resistant populations of Xanthomonas citri subsp. citri and epiphytic bacteria on citrus trees treated with copper or streptomycin using a new semi-selective medium

Cited by 37 publications

References 38 publications

Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos

Sensibilidad de bacterias procedentes de pasifloras a antibióticos y productos cúpricos

Hexyl gallate for the control of citrus canker caused by Xanthomonas citri subsp citri

Bifunctional peptides as alternatives to copper-based formulations to control citrus canker

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