Monitoring hairy-root growth by image analysis

Coles, Graeme D.; Abernethy, D. J.; Christey, M. C.; Conner, A. J.; Sinclair, Ben K.

doi:10.1007/bf02669285

Cited by 9 publications

(1 citation statement)

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“…Callus cultures were weighed and the area of medium covered with hairy roots was estimated by image analysis as described by Coles et al (1991). The relative growth of callus and hairy roots was determined as described above.…”

Section: Phytoxicity Of Miconazolementioning

confidence: 99%

Miconazole: An effective antifungal agent for plant tissue culture

Tynan

Conner

Macknight

et al. 1993

Plant Cell Tiss Organ Cult

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Miconazole at concentrations between 5 and 20 mg 1-1 inhibited hyphal growth and sporulation in a wide range of fungi commonly associated with plants. These fungi included 4 Oomycetes, 11 Deuteromycetes, 4 Ascomycetes and 4 common airborne contaminants of plant tissue culture. The phytotoxicity of 20 mg 1-1 miconazole was also tested against a wide range of in vitro plant cultures.Shoot cultures from 15 species showed either no response or a slight growth reduction in the presence of miconazole. Although the growth of shoot cultures in 2 other species was significantly reduced by miconazole, a positive growth rate was maintained. Callus and hairy root cultures from 5 species were more sensitive to miconazole than shoot cultures, although they also retained a positive growth rate in the presence of the antifungal agent. Reconstruction experiments demonstrated the effectiveness miconazole had for the rescue of plant material from in vitro cultures contaminated from fungi.

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Section: Phytoxicity Of Miconazolementioning

confidence: 99%