“…The near infrared spectroscopy (NIRS) is a very efficient method that has been widely used for high-throughput determine various chemical and biochemical structures of agricultural crop ( Washburn et al., 2013 ). For instances, NIRS has been used for high-throughput predicting fiber and nutrient content of dryland cereal cultivars ( Brenna and Berardo, 2004 ; Stubbs et al., 2010 ), phenotyping of moisture and amylose content in maize ( Wang et al., 2019 ; Dong et al., 2021 ), evaluating the composition of carbohydrates in soybean ( Leite et al., 2020 ; Singh et al., 2021 ), detecting biomass of plant root mixtures ( Roumet et al., 2006 ), analyzing available P contents in soils to aid fertilization ( Patzold et al., 2020 ), as well as determining the internal quality and physiological maturity in the fruit ( Cunha et al., 2016 ; de Carvalho et al., 2019 ; Minas et al., 2021 ). In our previous studies, the NIRS has been successfully applied for stalk quality determination ( Wang et al., 2021 ), cell wall features and lignocellulose digestibility characterization in sugarcane ( Li et al., 2021 ; Adnan et al., 2022 ).…”