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Cereals are an important source of food for millions of people across low-middle-income countries. Cereals are considered a staple food for poor people. The majority of the people are depending upon agricultural occupation. Agriculture provides a primary source of income for many farmers in low-middle-income countries. The pre- and post-harvest loss of crop yield affects farmers and is a major problem in achieving food security. Biotic and abiotic factors cause pre- and post-harvest loss of crop yield worldwide. It significantly affects the economic status of farmers as well as low-middle-income countries. Many advanced technologies are available for resolving the pre- and post-harvest loss of crop yield. The past few decades have seen remarkable progress in crop improvement. Especially high-throughput genome sequencing approaches contributed to advancement in the crop improvement. Genome-editing has also been considered a key tool for crop improvement. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become a potent genome editing system for modifying key traits in cereal crops. CRISPR/Cas9 system offers new opportunities for addressing pre-and post-harvest constraints affecting cereal grain production and storage. In this review, we discuss the application of the CRISPR/Cas9 genome editing system to reduce pre-and post-harvest yield loss in cereal crops. It may promote the economic status of farmers and reduce food demand in the coming decades.
Cereals are an important source of food for millions of people across low-middle-income countries. Cereals are considered a staple food for poor people. The majority of the people are depending upon agricultural occupation. Agriculture provides a primary source of income for many farmers in low-middle-income countries. The pre- and post-harvest loss of crop yield affects farmers and is a major problem in achieving food security. Biotic and abiotic factors cause pre- and post-harvest loss of crop yield worldwide. It significantly affects the economic status of farmers as well as low-middle-income countries. Many advanced technologies are available for resolving the pre- and post-harvest loss of crop yield. The past few decades have seen remarkable progress in crop improvement. Especially high-throughput genome sequencing approaches contributed to advancement in the crop improvement. Genome-editing has also been considered a key tool for crop improvement. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become a potent genome editing system for modifying key traits in cereal crops. CRISPR/Cas9 system offers new opportunities for addressing pre-and post-harvest constraints affecting cereal grain production and storage. In this review, we discuss the application of the CRISPR/Cas9 genome editing system to reduce pre-and post-harvest yield loss in cereal crops. It may promote the economic status of farmers and reduce food demand in the coming decades.
The article shows the results of a multi-environment trial which was conducted in 2022 to determine the adaptability of corn hybrids and identify genotypes which are better to choose for a short growing season. Ten new early-maturing (FAO 130-150) corn hybrids were studied in 6 plant breeding organisations – ecological sites in 4 soil-climatic zones of Russia such as the Central Black Earth region, North Caucasus, Urals, West Siberia. The corn hybrids were evaluated by plasticity (bi), stability (σd2) and selection indices: the breeding value of the cultivar (Svc), the selection index (Si) and the selection value index of the cultivar (Ssvi). Nalchik (Ij = +1.51), Belgorod (Ij = +0.18) and Voronezh (Ij = +0.04) had favourable environmental conditions, unfavourable environments were in Chelyabinsk (Ij = -1.09), Omsk (Ij = -0.56) and Pyatigorsk (Ij = -0.08). Hybrid 140/26 had the highest grain yield (6.50 t/ha) on average, it exceeded the best standard by 1.15 t/ha. The hybrids 140-28 had the highest mean yield (7.66 t/ha) and 140/24 (7.65 t/ha) under favourable conditions, hybrid 100/28 was the best (5.42 t/ha) in unfavourable environments. Hybrids 100/24 (18.7 %), 140/25 (19.0 %), 100/27 (20.5 %) had the lowest grain moisture at harvest. Hybrids 140/24 (bi = 1.82, σd2 = 0.08), 140/28 (bi = 1.54, σd2 = 0.09), 100/26 (bi = 1.45, σd2 = 0.17) with high ecological plasticity and stability were recommended to grow under intensive growing conditions. It was shown that the application of the technique for evaluating corn hybrids by selection indices makes it possible to identify regionally oriented genotypes. It is better to grow hybrids 100/28 (Svic = 8.44) и 100/27 (Svic = 8.26), which had an optimal balance of environmental stability, grain yield and grain moisture at harvest, in unfavourable conditions with a short growing season.
Abstract. The expansion of the northern zone of corn-sowing in the Russian Federation, the increase in demand for corn hybrids capable of maturing in zones with a short frost-free period, forming a high yield of grain and green mass, adapted to various soil and climatic zones, requires intensification of breeding work for early maturity. The purpose of the research is the creation of a new early-ripening corn hybrid of universal use, characterized by adaptability to various soil and climatic conditions. The research was carried out at the Agricultural Research Center “Donskoy” and research institutions in Russia in 2018–2021. Methods. The main method of creating hybrids is the method of interline hybridization using heterosis in the first generation. Topcross crosses were used to obtain hybrid combinations. Object of the study are early ripe corn hybrids. A new early-ripening corn hybrid Vityaz’ MV of universal use was created jointly by the ARC “Donskoy” and the Federal State Budgetary Scientific Institution of the National Grain Center named after. P. P. Lukyanenko. It is included in the State Register of Breeding Achievements and approved for use in the production of the North Caucasian, Central, Central Black Earth and Middle Volga regions for grain and silage. According to the research results, the new hybrid is distinguished by high grain yield and plasticity. In the environmental test of research institutions in Russia, located in different soil and climatic conditions, the grain yield was 5.03–10.30 t/ha. In the ARC “Donskoy”, the average grain yield, including dry years, was 4.31 t/ha, which is significantly (by 0.36 t/ha) higher than that of the Krasnodarskiy 194 MV standard. The yield of green mass – 28.2 t/ha, dry matter – 9.41 t/ha. The new hybrid is drought-resistant, resistant to lodging and bunt smut, and is characterized by low harvest grain moisture content (14.0 %). The scientific novelty should be considered the creation of a new early-ripening corn hybrid Vityaz’ MV of universal use, adapted to various soil and climatic zones of the Russian Federation.
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