Monitoring Neutralization Property Change of Evolving Hantaan and Seoul Viruses with a Novel Pseudovirus-Based Assay

Ning, Tingting; Liu, Shuo; Ma, Jian; Nie, Jianhui; Huang, Weijin; Li, Xuguang; Li, Yuhua; Wang, Youchun

doi:10.1007/s12250-020-00237-y

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…According to previous studies on the glycoprotein of hantavirus, it is found that SEOV has 8 mutation hotspots currently [ 18 ]. In this study, 13 mutant amino acids were found by comparing HN1 and HN4 with the international standard strain SEOV80-39 ( Table 6 ).…”

Section: Resultsmentioning

confidence: 99%

“…The eight mutations are common in SEOV, and the mutation frequency exceeds 40%, which is common among hantaviruses. Besides, we also found 6 variations of glycoprotein related to epitope, which may cause antigenicity change [ 18 ]. The tertiary structure of glycoprotein based on HN1 and HN4 M segment was constructed.…”

Section: Discussionmentioning

confidence: 99%

“…According to previous studies, the M segment is the fastest mutated segment of hantavirus, and the mutation of this segment can cause changes in virus virulence, antigenicity, and cell fusion [ 18 ]. Amino acid variations between HN1 and HN4 and SEOV 80–39 and vaccine strains were studied using DNAMAN software.…”

Section: Methodsmentioning

confidence: 99%

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Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China

et al. 2022

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Background Hantaviruses (HVs) are major zoonotic pathogens in China that cause hemorrhagic fever with renal syndrome (HFRS) posing a major threat to people’s health. Hainan Province, an island located in Southeast China, is an ideal region for sea ports. The unique tropical monsoon climate in Hainan provides sufficient living conditions for rodents, which help spread HVs and other rodent-borne diseases. In the routine monitoring of hantavirus, there was no evidence that rodents in Hainan carried hantavirus. No patients infected with hantavirus were found in the past. However, the surveillance of HVs-carrying rodents covering the whole territory of Hainan has not stopped. Methodology/principal findings For the monitoring of the prevalence of HVs in rodents and the search for theoretical reference for rodent control and HFRS prevention, a total of 60 rodents from 6 monitoring spots were trapped around main ports in Hainan between 2016 and 2019. HV positive samples were identified by a specific kit and sequenced. The data indicated that seven rodents (Rattus norvegicus) were positive for hantavirus with a positivity rate of 11.67%. Phylogenetic analysis suggested that the two complete sequence strains HN1 and HN4 in this research were highly similar to the sequence strains GZRn36 and GZRn148 isolated in Guangdong Province, and they located in the same phylogenetic tree branch which belongs to S2 subtype. Although the two partial sequences HT1 and HT2 isolated in Xisha Islands belong to S2 subtype according to the phylogenetic tree of L segment, they showed a great nucleotide difference with HN1 and HN4. We also found 13 amino acid variations compared with SEOV 80–39 and 6 amino acid mutations related to epitope, and the variations may reduce the effectiveness of the current HFRS vaccines used in humans. Conclusions/significance The study indicated HVs carried by rodents found in Hainan Province may be transmitted from Guangdong Province through trading ports and carriage of goods by sea. So it is of great significance to strengthen the surveillance of rodents in port areas especially capture and eliminate rodents on ship. Timely elimination of host animals of hantavirus in port areas is necessary to prevent an outbreak of HVs disease.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China

et al. 2022

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“…Hantaan virus (HTNV), the prototype hantavirus (belonging to the Bunyavirales order and Hantaviridae family) carried by Apodemus agrarius , is a widespread zoonotic virus with negative-sense RNA genomes. The segmented genome of HTNV is composed of small ( S ), medium ( M ) and large ( L ) parts, which encode the nucleocapsid protein (NP), glycoprotein precursor (GPC, further cleaved into the N-terminal Gn and C-terminal Gc), and RNA-dependent polymerase protein (RdRp), respectively ( Ning et al., 2021 ). HTNV is mainly transmitted to humans by infective aerosols from rodent excrements ( Park et al., 2021 ), which causes severe hemorrhagic fever with renal syndrome (HFRS).…”

Section: Introductionmentioning

confidence: 99%

RIPK3 promotes hantaviral replication by restricting JAK-STAT signaling without triggering necroptosis

Si,

Zhang,

Zhou

et al. 2023

Virologica Sinica

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“…Due to the characteristics of strong operability, low biological risk, convenient detection, and high sensitivity, pseudoviruses have been widely used in the research of highly pathogenic viruses, such as SARS ( Kobinger et al, 2007 ), MERS ( Fan et al, 2018 ), Ebola ( Liu et al, 2017 ), Influenza ( Lu and Jiang, 2013 ), Chikungunya ( Wu et al, 2017 ), Hantan and Seoul Viruses ( Ning et al, 2021 ), and especially in those newly discovered, high-infectious viruses. For example, during the outbreak of SARS-CoV-2, the research of live virus must be carried out in the biosafety level (BSL) 3 facilities, and mutant live viruses are very difficult to obtain.…”

Section: Introductionmentioning

confidence: 99%

Application of pseudovirus system in the development of vaccine, antiviral-drugs, and neutralizing antibodies

Xiang

et al. 2022

Microbiological Research

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Monitoring Neutralization Property Change of Evolving Hantaan and Seoul Viruses with a Novel Pseudovirus-Based Assay

Cited by 10 publications

References 33 publications

Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China

Genetic and hosts characterization of hantaviruses in port areas in Hainan Province, P. R. China

RIPK3 promotes hantaviral replication by restricting JAK-STAT signaling without triggering necroptosis

Application of pseudovirus system in the development of vaccine, antiviral-drugs, and neutralizing antibodies

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