Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

Hamedo, Hend

doi:10.2174/1874070700903010103

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…The antimicrobial effects of es-Evaluation of Addition of Plant Fixed Oil Extracts (Ginger , Maramia, Eucalyptus) on Some Properties of Heat Cured Denture Base Material sential oils have been documented and used in herbal medicine in many countries. (2) Ginger (Zingiberofficinale) is one of the most widely used species of ginger family .Ginger has long history of medicinal use dating back 2500 years in China and India. Recently,medical researchers have verified that ginger contains several bioactive constituents and possesses health promoting properties.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Addition of Plant Fixed Oil Extracts (Ginger , Maramia, Eucalyptus) on Some Properties of Heat Cured Denture Base Material

Al−Nema¹,

Al-Irhayim²,

Ali³

2014

RDENTJ

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The aim of this study was to evaluate the effect of three types of natural medicinal plants oils on the transverse strength, residual monomer and hardness of polymethylmethacrylate. Materials and methods: Total number of samples of (105) have been prepared in this study. Fifteen samples of heat cured ProBase acrylic resin were prepared without additives (Control), and (90) samples were prepared with additives (Ginger oil ,Meramia and Eucalyptus oil) in two concentrations (1.5 and 2.5) to evaluate transverse strength, indentation hardness and residual monomer. Results: The results showed a significant difference between control and addition groups in the transverse strength and hardness tests. The addition of oils decreased the transverse strength ,and increased the hardness.There was no significant difference between the residual monomer for control and addition groups, but the control group at the first day showed higher residual monomer release. Conclusion: The addition of eucalyptus , meramia and ginger oils to acrylic resin decreased the transverse strength , increased its hardness and showed no significant difference in residual monomer.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Addition of Plant Fixed Oil Extracts (Ginger , Maramia, Eucalyptus) on Some Properties of Heat Cured Denture Base Material

Al−Nema¹,

Al-Irhayim²,

Ali³

2014

RDENTJ

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“…Variations in RAPD profiles were reported by Hamedo ( 26 ) who studied the antimicrobial activity of the essential oils of Rosmarinus officinalis . The authors found that differences in RAPD profiles could be due to DNA damage after essential oils addition, although the differences could also be attributed to the oligonucleotide used ( 26 ). Changes in bands intensity were found in this study, suggesting no DNA damage.…”

Section: Discussionmentioning

confidence: 89%

Effect of the Essential Oil of Minthostachys verticillata (Griseb.) Epling and Limonene on Biofilm Production in Pathogens Causing Bovine Mastitis

et al. 2018

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Bovine mastitis causes large annual economic losses around the world. Different microorganisms are associated with the disease. The capacity of pathogens to adhere to bovine mammary epithelial cells is associated with biofilm production which leads to antibiotic resistance. Research is now leading to search alternative control methods and medicinal plants constitute a natural, safe, effective and inexpensive option. Minthostachys verticillata is an autochthonous medicinal plant of Argentina with multiple ethnobotanical properties. In a previous study, we demonstrated that the essential oil (EO) of this species and limonene, one of its compounds, inhibited the growth of mastitis pathogens. The objective of the present work was to determine the inhibitory effect of the essential oil of M. verticillata and limonene, on biofilm formation and on mature biofilm produced by pathogens isolated from bovine mastitis. Time kill assay and bacterial lysis were also determined. Furthermore, RAPD-PCR assays were performed to determine changes in bacterial DNA after EO and limonene exposition. Bacterial isolates were identified as Escherichia coli (EC3 and EC9), Bacillus pumilus (BP5, BP6, and BP7) and Enterococcus faecium (EF1) by rRNA 16S sequencing and MALDI-TOF MS. All the strains were able to form biofilm. Addition of both lactose and sucrose did not affect biofilm production. MIC values for EO were 3.6 mg/ml for E. faecium; 0.9 mg/ml for E. coli (EC3), 14.5 mg/ml for E. coli (EC9), 1.8 mg/ml for B. pumilus (BP7), 3.63 mg/ml for B. pumilus (BP6) and 29.0 mg/ml for B. pumilus (BP7). MIC values for limonene were 6.6 mg/ml for B. pumilus (BP6) and 105 mg/ml for B. pumilus (BP5). These results demonstrated that EO was more effective than limonene, showing also bactericidal action against E. faecium (minimal inhibitory concentration (MBC) = 29.0 mg/ml). This result was corroborated by time of death assay, observing a cell decrease after at 6 h, and then by bacterial lysis assay. Both EO and limonene affected mature biofilm of isolated strains. The results contribute to the study of EO and limonene which may serve as a therapy against bovine mastitis pathogens inhibiting the development of pathogenic bacteria.

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“…REO demonstrated activity against E. coli and Staph. aureus with 1.25 and 5 µmL, respectively [ 38 ]. Another study, by Bousbia et al, found that REO has antibacterial action against Staphylococcus aureus and Escherichia coli , with inhibition zones 12.5 and 15.5 mm, respectively [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Using Rosemary Essential Oil as a Potential Natural Preservative during Stirred-like Yogurt Making

Kamel

Mansour

El-diin

et al. 2022

Foods

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The popularity of rosemary has grown as a natural alternative over the synthetic supplements due to its potential health benefits. The rosemary plant has been utilized to preserve food due to its ability to prevent oxidation and microbial contamination. The reason for this study was to determine the phytochemical components and antimicrobial activity of rosemary essential oil (REO) and the effect of REO addition (0.5 and 0.7%) on the chemical, microbiological, and sensory properties of stirred-like yogurt (SLY) during 16 days of storage at 4 °C. The obtained data observed that REO exhibited antimicrobial action against Escherichia coli, Staphylococcus aureus, and Salmonella marcescens, as well as fungi (Aspergillus flavus) and yeasts (Candida albicans). Increased REO to 0.7% accelerated (p < 0.05) the development of lactic acid bacteria (LAB) in SLY (8.3 log cfu/g) and delayed yeast growth up to 12 days. Molds and coliforms were also not found in the SLY samples with REO. In comparison to control samples, sensory results showed that the addition of REO improves the overall acceptance of SLY (p < 0.05). In conclusion, the current study found that REO could be used as a natural preservative during the production of SLY to extend shelf-life and promote LAB development.

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Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

Cited by 7 publications

References 35 publications

Evaluation of Addition of Plant Fixed Oil Extracts (Ginger , Maramia, Eucalyptus) on Some Properties of Heat Cured Denture Base Material

Evaluation of Addition of Plant Fixed Oil Extracts (Ginger , Maramia, Eucalyptus) on Some Properties of Heat Cured Denture Base Material

Effect of the Essential Oil of Minthostachys verticillata (Griseb.) Epling and Limonene on Biofilm Production in Pathogens Causing Bovine Mastitis

Using Rosemary Essential Oil as a Potential Natural Preservative during Stirred-like Yogurt Making

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