Monitoring of Basophil Activation Using CD63 and CCR3 in Allergy to Muscle Relaxant Drugs

Monneret, Guillaume; Benoit, Yves; Debard, Anne Lise; Gutowski, Marie-Claude; Topenot, Ingrid; Bienvenu, Jacques

doi:10.1006/clim.2001.5156

Cited by 114 publications

(96 citation statements)

References 35 publications

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“…The sensitivity of CD63 neo-expression for reactivity to muscle relaxants was better than in the two previously reported studies [4,5]. The sensitivity for CD203c was only 36% but specificity was 100%.…”

Section: Discussioncontrasting

confidence: 61%

“…When cells were stained with CD45-CY5, IgE-FITC and CD63-PE (or CD203c) monoclonal antibodies with gating on the anti-CD45 positive population and the IgE-positive population results were considered positive if greater than 10% of this subpopulation stained with anti-CD63 or anti-CD203c (with < 5% staining in the control group). Results were considered positive only if the two dilutions of the drug demonstrated greater than 10% positivity, the criterion used by Monneret et al [5].…”

Section: Flow Cytometrymentioning

confidence: 99%

“…The most common basophil activation marker used in current research is the neo-expression of CD63. CD63, an activation marker on basophils, has been used as a reporter molecule of the state of activation produced by a large number of allergens [2,3] and neuromuscular blocking drugs [4,5]. In both the latter studies, the specificity has been shown to be high (93% and 100%) and the sensitivity low (63% and 54%).…”

mentioning

confidence: 99%

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Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

et al. 2005

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SummaryThe investigation of anaphylactic reactions in the peri-operative period is difficult. Elevation of serum tryptase levels is a good indicator of an anaphylactic event but the ability of subsequent investigations to identify the drug(s) responsible for the reaction is still potentially unreliable. The aim of this study was to examine basophil activation as an investigative tool. We performed flow cytometric analysis of the expression on the cell surface of the basophil activation markers CD63 and CD203c and measured histamine release in 21 patients who were referred with possible peri-operative anaphylaxis. The sensitivity of CD63, CD203c, basophil histamine release and skin prick for the muscle relaxants was found to be 79%, 36%, 36% and 64%, respectively; the specificity was found to be 100%. These results demonstrate the difficulty in investigating the cause of an unexpected clinical event following drug administration, but the higher sensitivity of neo-expression on the cell surface of CD63 suggests that flow cytometric analysis of its neo-expression on basophils in vitro may be a diagnostic aid.

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Section: Discussioncontrasting

confidence: 61%

Section: Flow Cytometrymentioning

confidence: 99%

mentioning

confidence: 99%

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Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

et al. 2005

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“…Relevant studies have found that BAT sensitivity and specificity for muscle relaxants varied from 36% to 92% and 93% to 100%, respectively (Sudheer et al, 2005). Most of these studies reported a high specificity and a sensitivity above 50%, reaching 91% (Abuaf et al, 1999;Kvedariene et al, 2006;Laxenaire et al, 1999;Monneret et al, 2002;Moss, 1995;Stellato et al, 1991). This sensitivity is lower (36%) when the marker CD203c is used (Sudheer et al, 2005).…”

Section: Basophil Activation Test In Muscle Relaxant Allergic Reactionsmentioning

confidence: 99%

“…Numerous studies over recent years have shown the usefulness of this technique for the in vitro diagnosis of allergic diseases (Ebo et al, 2006a(Ebo et al, , 2004aKleine-Tebbe et al, 2006) involving different allergens, such as inhalants (Paris-Köhler et al, 2000;Saporta et al, 2001), hymenoptera venoms (Sabbah et al, 1998;Sainte-Laudy et al, 2000), or latex (Ebo et al, 2002;Sanz et al, 2003). For the diagnosis of allergy to drugs like muscle relaxants (Abuaf et al, 1999;Monneret et al, 2002), betalactam antibiotics (Sanz et al, 2001b(Sanz et al, , 2002aTorres et al, 2004), pyrazolones (Sabbah et al, 1997;Sanz et al, 2005) and non steroidal anti-inflammatory drugs (NSAIDs) BAT has opened up an important diagnostic pathway, either on its own or combined with other techniques (de Weck et al, 2008b;Gamboa et al, 2003a), allowing avoidance of challenge tests in an important number of cases (Hausmann et al, 2009;Sanz et al, 2009).…”

Section: Introductionmentioning

confidence: 99%