2007
DOI: 10.1002/elps.200700332
|View full text |Cite
|
Sign up to set email alerts
|

Monitoring of enzymatic reactions using conventional and on‐chip capillary electrophoresis with contactless conductivity detection

Abstract: The use of CE with contactless conductivity detection was evaluated for monitoring enzymatic reactions. The nonionic species ethanol, glucose, ethyl acetate, and ethyl butyrate were made accessible for analysis by CE via charged products or by-products obtained in enzymatic conversions using hexokinase, glucose oxidase, alcohol dehydrogenase, and esterase. Two of the reactions, namely the conversion of glucose with glucose oxidase and that of ethylacetate with esterase, were also successfully demonstrated on a… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
12
0

Year Published

2008
2008
2013
2013

Publication Types

Select...
6
2

Relationship

3
5

Authors

Journals

citations
Cited by 20 publications
(12 citation statements)
references
References 31 publications
0
12
0
Order By: Relevance
“…Conventional CE and MCE with C 4 D were evaluated for monitoring enzymatic reactions (Schuchert‐Shi et al. , 2007, 2008). The nonionic species ethanol, glucose, ethyl acetate and ethyl butyrate were made accessible for analysis by CE.…”
Section: Application Of Contactless Conductivity Detection In Ce and Mcementioning
confidence: 99%
“…Conventional CE and MCE with C 4 D were evaluated for monitoring enzymatic reactions (Schuchert‐Shi et al. , 2007, 2008). The nonionic species ethanol, glucose, ethyl acetate and ethyl butyrate were made accessible for analysis by CE.…”
Section: Application Of Contactless Conductivity Detection In Ce and Mcementioning
confidence: 99%
“…The nonionic species glucose, ethanol, ethyl acetate, and ethyl butyrate were made accessible for analysis by CE via charged products or byproducts obtained in enzymatic conversions using hexokinase, glucose oxidase, alcohol dehydrogenase, and esterase, respectively. 18 Recently, urea was also determined by CE-C 4 D in clinical samples via enzymatic conversion to ammonium ion with urease. 19 The application of CE-C 4 D to the monitoring of the lipase-catalyzed hydrolysis of amino acid esters is reported herein.…”
Section: -11mentioning
confidence: 99%
“…Quantification was carried out by conductivity measurement. The use of CE-C 4 D for the monitoring of enzymatic reactions has been demonstrated for conversions of ethanol, glucose, ethyl acetate and ethyl butyrate [30]. Enzymatic conversion of urea to ammonium by conventional or microchip CE-C 4 D has also been described [31].…”
Section: Introductionmentioning
confidence: 98%