Monitoring of GMO in Brazilian processed meat and soy-based products from 2007 to 2008

Dinon, Andréia Zilio; Treml, Diana; Mello, Carla Souza de; Arisi, Ana Carolina Maisonnave

doi:10.1016/j.jfca.2009.12.002

Cited by 27 publications

(14 citation statements)

References 24 publications

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“…The polymerase chain reaction (PCR) is the analysis which is most used in the detection of GMOs, due to its high sensitivity and specificity in DNA amplification (DINON et al, 2010;MARMIROLI et al, 2008;MICHELINI et al, 2008). Furthermore, it is an important tool in the case of a re-test, when there is doubt about the detection of transgenic organisms by other methodologies (CUNHA et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Toothpick test: a methodology for the detection of RR soybean plants

Silva

Unêda-Trevisoli

Villela

et al. 2015

Revista Ciência Agronômica

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-Due to the large increase in the area cultivated with genetically modified soybean in Brazil, it has become necessary to determine methods that are fast and efficient for detecting these cultivars. The aim of this work was to test the efficiency of the toothpick method in the detection of RR soybean plants, as well as to distinguish between cultivars, for sensitivity caused by herbicide. Ten transgenic soybean cultivars, resistant to the active ingredient glyphosate, and ten conventional soybean cultivars were used. Toothpicks soaked in glyphosate were applied to all the plants at stage V6 and evaluations were made at 2, 4, 6, 8 and 10 days after application (DAA). The effects of the glyphosate on the cultivars, and the symptoms of phytotoxicity caused in the transgenic plants were evaluated by means of grading scales. The toothpick test is effective in identifying RR soybean cultivars and also in separating them into groups by sensitivity to the symptoms caused by the glyphosate.Key words: Glycine max. Phytotoxicity. Glyphosate. Toothpick method. Herbicide.RESUMO -Devido ao grande aumento da área cultivada com soja geneticamente modificada no Brasil, faz-se necessária a determinação de métodos que sejam rápidos e eficientes na detecção dessas cultivares. O objetivo deste trabalho foi testar a eficiência do método do palito na detecção de plantas de soja RR, bem como distinguir as cultivares quanto à sensibilidade causada pelo herbicida. Foram utilizadas dez cultivares de soja transgênica, resistentes ao ingrediente ativo glifosato, e dez cultivares de soja convencional. Palitos de dente embebidos em glifosato foram aplicados em todas as plantas em estádio V6 e as avaliações foram realizadas aos 2; 4; 6; 8 e 10 dias após a aplicação (DAA). Os efeitos da ação do glifosato nas cultivares e os sintomas de fitotoxicidade, ocasionados nas plantas transgênicas, foram avaliados por meio de escalas de notas. O teste do palito é eficiente para identificar as cultivares de soja RR e, além disso, separá-las em grupos de sensibilidade aos sintomas causados pelo glifosato.Palavras-chave: Glycine max. Fitotoxidade. Glifosato. Método do palito. Herbicida.

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Section: Resultsmentioning

confidence: 99%

Toothpick test: a methodology for the detection of RR soybean plants

Silva

Unêda-Trevisoli

Villela

et al. 2015

Revista Ciência Agronômica

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“…There are some published studies about the detection and quantification of the amount of transgenic material present in Brazilian processed food (BRANQUINHO; FERREIRA; CARDARELLI-LEITE, 2010;BROD et al, 2007;BROD;CARDARELLI et al, 2005;DE MELO;, DINON, 2010FERREIRA;BRANQUINHO;CARDARELLI-LEITE, 2009;GREINER;KONIETZNY, 2008;MARCELINO;GUIMARÃES;BARROS, 2007), but none of these products that contain modified GMO above the threshold level had been appropriately labeled, reinforcing the need for continous analyses in processed food products.…”

Section: Dna Concentration Yield and Puritymentioning

confidence: 99%

Use of real-time PCR to evaluate two DNA extraction methods from food

2012

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The DNA extraction is a critical step in Genetically Modified Organisms analysis based on real-time PCR. In this study, the CTAB and DNeasy methods provided good quality and quantity of DNA from the texturized soy protein, infant formula, and soy milk samples. Concerning the Certified Reference Material consisting of 5% Roundup Ready® soybean, neither method yielded DNA of good quality. However, the dilution test applied in the CTAB extracts showed no interference of inhibitory substances. The PCR efficiencies of lectin target amplification were not statistically different, and the coefficients of correlation (R²) demonstrated high degree of correlation between the copy numbers and the threshold cycle (Ct) values. ANOVA showed suitable adjustment of the regression and absence of significant linear deviations. The efficiencies of the p35S amplification were not statistically different, and all R² values using DNeasy extracts were above 0.98 with no significant linear deviations. Two out of three R² values using CTAB extracts were lower than 0.98, corresponding to lower degree of correlation, and the lack-of-fit test showed significant linear deviation in one run. The comparative analysis of the Ct values for the p35S and lectin targets demonstrated no statistical significant differences between the analytical curves of each target.

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“…GM labelling laws in response to many countries' threshold legislation are required to ensure commercial food safety and quality. These laws stipulate that GM containing foods above 1% must be clearly labelled (Busch et al, 2001;Dinon et al, 2010Hamels et al, 2009;Brazil, 2003;EC, 49/2000EC, 49/ , 1139EC, 49/ /1998Robinson, 1999). The objective of this study was thus to screen GM materials in food and feed products containing maize or soy and commercially available in the Jordanian market in 2007 and 2008. samples) used in the present study were randomly purchased from local supermarkets in cities located in Amman and Aqaba.…”

Section: Introductionmentioning

confidence: 99%

Detection of genetically modified material in feed and foodstuffs containing soy and maize in Jordan

Herzallah¹

2012

Journal of Food Composition and Analysis

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Monitoring of GMO in Brazilian processed meat and soy-based products from 2007 to 2008

Cited by 27 publications

References 24 publications

Toothpick test: a methodology for the detection of RR soybean plants

Toothpick test: a methodology for the detection of RR soybean plants

Use of real-time PCR to evaluate two DNA extraction methods from food

Detection of genetically modified material in feed and foodstuffs containing soy and maize in Jordan

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