Monitoring production process of cisplatin‐loaded PLGA nanoparticles by FT‐IR microspectroscopy and univariate data analysis

Portaccio, Marianna; Menale, Ciro; Diano, Nadia; Serri, Carla; Mita, D.G.; Lepore, Maria

doi:10.1002/app.41305

Cited by 25 publications

(17 citation statements)

References 48 publications

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“…The characteristic absorbance wavelengths obtained for DEX, PLGA fibers and DEX-loaded PLGA fibers are in good agreement with those previously reported in literature. [56][57][58] We hypothesized that the alignment of the fibers would have an effect on the release of encapsulated DEX. To assess this hypothesis, first the structural and thermal properties of both random and aligned fibers were analyzed using XRD and DSC techniques, respectively.…”

Section: Conical Ratio = D 2 /D 1 (Equation 1)mentioning

confidence: 99%

Electrospinning of highly aligned fibers for drug delivery applications

Eslamian

Khorrami

et al. 2019

J. Mater. Chem. B

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Section: Conical Ratio = D 2 /D 1 (Equation 1)mentioning

confidence: 99%

Electrospinning of highly aligned fibers for drug delivery applications

Eslamian

Khorrami

et al. 2019

J. Mater. Chem. B

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“…A weak peak at 1620 cm −1 could be assigned to amide groups (Cipriani et al 2016;Li et al 2013). Also, the band observed near 1110 cm −1 is possibly due to the C−H rocking mode of CH 3 groups of any residual DMF solvent (Portaccio et al 2015). The peak that appeared at 1620 cm −1 is likely due to the O−H bending of chitosan (Kakida & Tashiro 1997;Xue et al 2013).…”

Section: Ftir Analysismentioning

confidence: 94%

An Enhanced Safrole Sensing Performance of a Polyacrylonitrile Nanofiber- Based-QCM Sensor by Overlaying with Chitosan

Rianjanu¹,

Triyana²,

Nurbaiti³

et al. 2019

JSM

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We report on a method to enhance the sensing performance of polyacrylonitrile (PAN) nanofiber-based QCM sensor overlaid with chitosan. The PAN nanofibers were deposited on the QCM sensing surface by electrospinning technique followed by overlay with chitosan by a drop-casting method. The Fourier transform infrared (FTIR) spectra confirm that chitosan covers the PAN nanofibers. The SEM images show the average diameter of the produced PAN nanofibers was 236 nm, and it increased to 283 nm after overlay with chitosan. The modified QCM sensor has the sensitivity of 18.7 Hz mg -1 L, which is better than that of PAN nanofiber alone of 4.5 Hz mg -1 L. It is an increase nearly 5 times. The analytical parameters of the limit of detection (LOD), sensitivity, a time constant, and stability improved after the PAN nanofiber sensor was overlaid with chitosan. The amine groups present in chitosan interact effectively with safrole, thus increase the sensing response. The proposed device is robust, inexpensive, and convenient for detecting safrole, and can be used as an alternative to those of classical instrumental methods for the analysis of safrole as a drug precursor. ABSTRAK Kajian ini melaporkan tentang kaedah untuk meningkatkan prestasi pengesanan poliakrilonitril (PAN) berasaskan sensor gentian nano QCM yang disaluti dengan kitosan. PAN nanofiber diendap pada permukaan pengesan QCM oleh teknik putaran elektro diikuti dengan salutan kitosan melalui kaedah drop-pemutus. Spektrum transformasi Fourier inframerah (FTIR) mengesahkan kitosan menutup gentian nano PAN. Imej SEM menunjukkan diameter purata gentian nano PAN yang dihasilkan adalah 236 nm dan ia meningkat kepada 283 nm selepas bertindih dengan kitosan. Sensor QCM terubah suai mempunyai kesensitifan 18.7 L mg -1 Hz, lebih baik daripada gentian nano PAN sahaja iaitu 4.5 Hz mg -1 L. Ini merupakan peningkatan hampir 5 kali ganda. Analisis parameter had pengesanan (LOD), kesensitifan, pemalar masa dan kestabilan bertambah baik selepas sensor gentian nano PAN disaluti dengan kitosan. Golongan amina yang hadir dalam kitosan berinteraksi secara berkesan dengan safrola, sekaligus meningkatkan respons pengesanan. Peranti cadangan ini teguh, murah dan mudah untuk mengesan safrola dan boleh digunakan sebagai alternatif kepada metod instrumen klasik untuk menganalisis safrola sebagai pelopor dadah.

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“…The peak of cisplatin (NH) was found at 1299 cm -1 . The amine peaks of cisplatin were seen at 1625 cm -1 (NHN) for asymmetric bending, 1316 cm -1 (NHN) for symmetric bending, and 1299 cm -1 (NHN) for symmetric bending [35]. Although GE and GE-Pt NPs had EGCG and cisplatin incorporated, the EGCG and cisplatin amount in total gelatin-based NPs was too low to be detected and distinguished by FTIR.…”

Section: Characterization Of Dual Drug-loaded Nanoparticles (Ge-pt Nps)mentioning

confidence: 96%

The Synergistic Anticancer Effect of Dual Drug- (Cisplatin/Epigallocatechin Gallate) Loaded Gelatin Nanoparticles for Lung Cancer Treatment

Chen

Wang

et al. 2020

Journal of Nanomaterials

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Lung cancer has the highest mortality of any cancer worldwide, and cisplatin is a first-line chemotherapeutic agent for lung cancer treatment. Unfortunately, cisplatin resistance is a common cause of therapeutic failure. The ability to overcome chemoresistance is crucial to the effective treatment of lung cancer. Recently, epigallocatechin gallate (EGCG), a type of polyphenol extracted from tea, has been shown to suppress the rapid proliferation of cancer cells, including lung cancer. We tested whether nanoparticles (NPs) carrying a dual drug load, cisplatin and EGCG, could overcome chemoresistance to cisplatin, by working together to kill lung cancer cells. Self-assembling gelatin/EGCG nanoparticles (GE) were synthesized, and cisplatin was then incorporated, to construct a dual drug nanomedicine (EGCG/cisplatin-loaded gelatin nanoparticle, named as GE-Pt NP). The particle size and zeta potential were examined by dynamic light scattering (DLS). The morphological structure of GE-Pt NPs was observed by transmission electron microscopy (TEM). In vitro testing was performed using a human lung adenocarcinoma cell line (A549). A cytotoxicity examination was performed, using a WST-8 cell proliferation assay. Intracellular cisplatin content was quantified by inductively coupled plasma mass spectrometry (ICP-MS). In conclusion, we successfully prepared GE-Pt NPs, as spherical structures, approximately 75 nm in diameter, with a positive charge (+19.83±0.25 mV). The encapsulation rate of cisplatin in GE-Pt was about 63.7%, and the EGCG loading rate was around 89%. A relatively low concentration of GE-Pt NPs (EGCG 5 μg/mL : cisplatin 2 μg/mL) exhibited significant cytotoxicity, compared to cisplatin alone. The GE-Pt NPs are freely taken up by cells via endocytosis, raising the intracellular cisplatin concentration to a therapeutic level. We consider that combination therapy of cisplatin and EGCG in nanoparticles (GE-Pt NPs) may help overcome cisplatin resistance and could effectively be used in the treatment of lung cancer.

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Monitoring production process of cisplatin‐loaded PLGA nanoparticles by FT‐IR microspectroscopy and univariate data analysis

Cited by 25 publications

References 48 publications

Electrospinning of highly aligned fibers for drug delivery applications

Electrospinning of highly aligned fibers for drug delivery applications

An Enhanced Safrole Sensing Performance of a Polyacrylonitrile Nanofiber- Based-QCM Sensor by Overlaying with Chitosan

The Synergistic Anticancer Effect of Dual Drug- (Cisplatin/Epigallocatechin Gallate) Loaded Gelatin Nanoparticles for Lung Cancer Treatment

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