Monitoring sãto paulo state rivers in brazil for mutagenic activity using the ames test

Valent, G.U; Sato, Maria Inês Z.; Cristina, María; Coelho, Letícia; Coimbrão, Carlos Alberto; Sanchez, Petra Sanchez; Martins, Maria Therezinha; Bonatelli, Renato

doi:10.1002/tox.2530080403

Cited by 30 publications

(9 citation statements)

References 15 publications

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“…The fact that the samples from the dry season were the most mutagenic corroborates with the conclusion that the industrial discharges are in fact the main drivers of the mutagenicity of Piracicaba River. When mutagenicity is higher in the wet season it can be related to runoff and not to point sources discharges, which is the case of our study (Valent et al, 1993).…”

Section: Chemical Analysismentioning

confidence: 55%

Quantifying the contribution of dyes to the mutagenicity of waters under the influence of textile activities

Vacchi

Vendemiatti

Silva

et al. 2017

Science of The Total Environment

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The combination of chemical analyses and bioassays allows the identification of potentially mutagenic compounds in different types of samples. Dyes can be considered as emergent contaminants and were detected in waters, under the influence of textile activities. The objective of this study was to evaluate the contribution of 9 azo dyes to the mutagenicity of representative environmental samples. Samples were collected along one year in the largest conglomerate of textile industries of Brazil. We analyzed water samples from an important water body, Piracicaba River, upstream and downstream two main discharges, the effluent of a wastewater treatment plant (WWTP) and the tributary Quilombo River, which receives untreated effluent from local industries. Samples were analyzed using a LC-MS/MS and tested for mutagenicity in the Salmonella/microsome microsuspension assay with TA98 and YG1041. Six dyes were detected in the collected samples, Disperse Blue 291, Disperse Blue 373, Disperse Orange 30, Disperse Red 1, Disperse Violet 93, and Disperse Yellow 3. The most sensitive condition for the detection of the mutagenicity was the strain YG1041 with S9. The concentration of dyes and mutagenicity levels varied along time and the dry season represented the worst condition. Disperse Blue 373 and Disperse Violet 93 were the major contributors to the mutagenicity. We conclude that dyes are contributing for the mutagenicity of Piracicaba River water; and both discharges, WWTP effluent and Quilombo River, increase the mutagenicity of Piracicaba River waters in about 10-fold. The combination of chemical analysis and bioassays were key in the identification the main drivers of the water mutagenicity and allows the selection of priority compounds to be included in monitoring programs as well for the enforcing actions required to protect the water quality for multiple uses.

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Section: Chemical Analysismentioning

confidence: 55%

Quantifying the contribution of dyes to the mutagenicity of waters under the influence of textile activities

Vacchi

Vendemiatti

Silva

et al. 2017

Science of The Total Environment

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“…The mutagenic index (MI) was also calculated for each dose as the ratio of average numbers of revertants per plate in a sample and in the negative control (solvent). A sample was considered mutagenic when the MI value was equal to or greater than 2 for at least one of the tested doses and if it had a reproducible dose‐response curve [29].…”

Section: Methodsmentioning

confidence: 99%

Mutagenicity assessment of produced water during photoelectrocatalytic degradation

Nie

et al. 2007

Enviro Toxic and Chemistry

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Oilfield produced water was treated by photocatalysis, electro‐oxidation, and photoelectrocatalysis, respectively. The chemical composition and toxicity of the raw effluent and treated products were assessed by chemical and mutagenicity analysis. The raw effluent exhibited mutagenic activity in both strains of Salmonella typhimurium. The lowest concentration of the dichloromethane extract capable of inducing a positive response in strains TA98 and TA100 were as low as 4 and 5 μg/plate, respectively. All three technologies could detoxify direct‐acting mutagenic organic pollutants efficiently, although they could not completely eliminate mutagenicity in the water after 60 min of treatment. At equivalent doses, photoelectrocatalysis exhibited the greatest capability to reduce genotoxicity, whereas photocatalysis was the least effective and did not cause appreciable change in mutagenicity. The gas chromatography—mass spectrometry (GC‐MS) analysis revealed that n‐alkanes (259.4 ng/L) and phenolic compounds (2,501.4 ng/L) were the main organic constituents in the oilfield produced water. Thus, the results of both biological and chemical analysis indicate that photoelectocatalysis was the most effective technology for degradation of oilfield wastewater.

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“…The mutagenic index (MI) was calculated for each dose; that is, the average number of revertants per plate divided by the average number of revertants per plate in the negative control. A sample was considered positive when MI 2 for at least one of the tested doses and there was a reproducible dose-response curve (Valent et al, 1993;Varella et al, 2004).…”

Section: Mutagenicity Assaymentioning

confidence: 99%

Mutagenicity and anti-mutagenicity of Acanthopanax divaricatus var. albeofructus

et al. 2011

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-The beneficial effects of Acanthopanax divaricatus var. albeofructus (ADA) extracts have been assessed by mutagenic and anti-mutagenic activities by Ames test. Mutation of Salmonella typhimurium strains TA 98, TA 100, TA1535, TA1537, and Escherichia coli WP2 uvr A was assayed in duplicates by the procedure of Maron and Ames in the presence or absence of S9 mix. As a result, ADA extracts were not mutagenic for S. typhimurium strains TA 98, TA 100, TA1535, TA1537, and E. coli by the Ames assay. Anti-mutagenic activity was assayed by the Ames mutagenicity assay using histidine mutant of S. typhimurium strains TA 98 and TA 100, using the plate-incorporation method. 2-Aminoanthrancene (2-AA), 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2), and sodium azide (NaN 3 ) were used as the mutagens. ADA extracts showed a strong anti-mutagenic activity against 2-AA-induced mutagenesis which requires liver-metabolizing enzymes, and the same extract exhibited inhibitory effects on AF-2 and NaN 3 -induced mutagenesis in the absence of liver-metabolizing enzymes. The data indicate that ADA extracts contain anti-mutagenic activities against typical mutagens. The anti-mutagenic property of ADA provides additional health supplemental value to the other claimed therapeutic properties of the plant.

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Monitoring sãto paulo state rivers in brazil for mutagenic activity using the ames test

Cited by 30 publications

References 15 publications

Quantifying the contribution of dyes to the mutagenicity of waters under the influence of textile activities

Quantifying the contribution of dyes to the mutagenicity of waters under the influence of textile activities

Mutagenicity assessment of produced water during photoelectrocatalytic degradation

Mutagenicity and anti-mutagenicity of Acanthopanax divaricatus var. albeofructus

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