Monitoring the death of single BaF3 cells under plasmonic photothermal heating induced by ultrasmall gold nanorods

Abstract

Morphological changes and trypan-blue staining are temporally tracked in single cells via optical microscopy after plasmonic photothermal heating.

Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands

“…51,52 Simultaneously, the localized plasmonic heat of AuNIs causes both local phase transition and photoporation in the directly contacted lipid bilayer. 53,54 The nanopores and fluid phase, i.e., the lateral expansion between phospholipids and lower coverage density, abruptly increase the membrane permeability and trigger cell swelling 32 due to water inflow, thereby releasing the genetic materials after the membrane rupture (Figure 1b).…”

“…Photothermal heating is actively utilized for diverse theragnostic applications such as cancer therapy, , pathogens inactivation, − and nucleic acid amplification, − including cell lysis. Photothermal heating exhibits attractive benefits such as ultrafast heating and cooling, local heat management, and specific target constituent damage using assorted light-absorbing materials such as metallic thin films or nanoparticles (NPs). , In particular, Au-coated polycarbonate membrane rapidly ruptures the membrane proteins of infectious pathogens for point-of-care urinalysis .…”

“…The membranes of cells are directly exposed to photothermal heat, which causes highly efficient membrane rupture without any chemical reagent and thus rapidly releases the genetic materials (Figure a). Photothermal heat increases the overall temperature inside the microfluidic chamber, inducing a phase transition of the lipid bilayer from gel to disordered fluid. , Simultaneously, the localized plasmonic heat of AuNIs causes both local phase transition and photoporation in the directly contacted lipid bilayer. , The nanopores and fluid phase, i.e., the lateral expansion between phospholipids and lower coverage density, abruptly increase the membrane permeability and trigger cell swelling due to water inflow, thereby releasing the genetic materials after the membrane rupture (Figure b).…”

Controlled Growth of Gold Nanoparticles on Covellite Copper Sulfide Nanoplatelets for the Formation of Plate–Satellite Hybrid Structures

“…Typically, these LSPRs occur in the visible and ultraviolet spectral regions. − The number and position of LSPR maxima in the spectrum can be adjusted by the shape, size, and material of the particles. − The resulting hot electrons can either relax by locally heating the nanoparticle or can be nonthermally transferred to the surrounding environment ( i.e. , surface-adsorbed molecules or neighboring semiconductors), − making them useful for a variety of applications, such as surface-enhanced Raman spectroscopy, imaging, sensing, plasmonic photothermal therapy, and catalysis. , …”